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Embryo production in defined sequential media and survival following vitrification in a sealed system

Published online by Cambridge University Press:  23 November 2017

V C Moreira ,
A Ainslie ,
K Mackie ,
M J A Mylne ,
M J Wood  and
T G McEvoy
V C Moreira*
Affiliation:
Scottish Agricultural College, Sustainable Livestock Systems Group, Aberdeen, United Kingdom
A Ainslie
Affiliation:
Scottish Agricultural College, Sustainable Livestock Systems Group, Aberdeen, United Kingdom
K Mackie
Affiliation:
Scottish Agricultural College, Sustainable Livestock Systems Group, Aberdeen, United Kingdom
M J A Mylne
Affiliation:
Britbreed Ltd, Ormiston, East Lothian, United Kingdom
M J Wood
Affiliation:
University of Aberdeen, Assisted Reproduction Unit, Aberdeen, United Kingdom
T G McEvoy
Affiliation:
Scottish Agricultural College, Sustainable Livestock Systems Group, Aberdeen, United Kingdom
*
Email: [email protected]
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Extract

Conventional in vitro embryo production and cryopreservation strategies applicable to livestock could benefit from further enhancement of biosecurity features. In particular, there is scope for a new generation of sequential culture media that avoid ill-defined constituents but closely match changing embryo needs. Likewise, complementary cryopreservation protocols using sealed containers would be beneficial, if they emulate or surpass existing embryo storage and subsequent survival standards. This study compared blastocyst yields from zygotes incubated in a conventional monoculture medium or in an alternative defined 2-stage sequential media system, with or without Trolox, a water-soluble antioxidant, and subsequently investigated blastocyst survival following vitrification in sealed CryoTips.

Type
Poster presentations
Information
Proceedings of the British Society of Animal Science , Volume 2006 , March 2006 , pp. 148
Copyright
Copyright © 2016 The American Society of International Law

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References

Moreira, V.C., McCallum, G.J., Ainslie, A. and McEvoy, T.G. 2005. Blastocyst production from bovine oocytes vitrified in a closed (biosecure) system following in vitro maturation in the presence or absence of vitamin E. Reproduction, Fertility and Development 17, 196-197.Google Scholar