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Published online by Cambridge University Press: 20 November 2017
Glycerol is the most commonly used protective substance in diluents for freezing ram semen (Salamon and Maxwell, 2000). Colas (1975) examined two concentrations of 2 and 4% glycerol for freezing ram semen and recommended an optimal level of 4% glycerol, although it may be slightly toxic to spermatozoa. The optimum concentration of glycerol for freezing ram semen is also influenced by type of diluent (Salamon and Maxwell, 2000). The objective of this study was to investigate the effect of three levels of glycerol 3, 4 and 5% and two types of diluents on motility and live rates of Baluchi ram spermatozoa.