Hostname: page-component-669899f699-ggqkh Total loading time: 0 Render date: 2025-04-30T01:53:21.278Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of increasing substrate concentration on the gas production profiles of pure maize starch and milled, high temperature dried grass

Published online by Cambridge University Press:  05 November 2021

J. A. Huntington  and
D. I. Givens
J. A. Huntington
Affiliation:
Feed Evaluation and Nutritional Sciences, ADAS Dairy Research Centre, Drayton, Alcester Road, Stratford-upon-Avon CV37 9RQ, UK
D. I. Givens
Affiliation:
Feed Evaluation and Nutritional Sciences, ADAS Dairy Research Centre, Drayton, Alcester Road, Stratford-upon-Avon CV37 9RQ, UK
Get access

Extract

Theodorou et al. (1994) using a manual pressure transducer technique observed a linear response to substrate concentration (cone.) in gas production (GP; ml absolute) whilst lag time and rate of GP values remained constant. This experiment investigated the effect of increasing the cone, of maize starch (S) and milled (1mm) dried grass (DG) on the GP profile using an automated GP technique (Cone 1994).

Either 0.1, 0.25, 0.5, 1.0 or 2.0 g of S or DG were weighed into 250 ml bottles and pre-soaked with anaerobic buffer (85 ml). The bottles were connected to a data logger/pressure tranducer unit and gassed with carbon dioxide. Incubation (39°C) started ∽16 h later when strained rumen fluid was injected into each bottle (15 ml) and a data logger initiated. When GP had ceased the final pH of each bottle was measured and 25 ml of 1M H3PO4 added to stop further enzymic activity. DG bottles were filtered to determine organic matter (OM) residue and hence OM degraded (OMD).

Type
Techniques
Information
Proceedings of the British Society of Animal Science , Volume 1996 , March 1996 , pp. 222
Copyright
Copyright © The British Society of Animal Science 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

France, J., Dhanoa, M.S., Theodorou, M.K., Lister, S.J., Davies, D.R. and Issac, D. (1993). A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. J. Theor. Biol. 163: 99–111.10.1006/jtbi.1993.1109CrossRefGoogle Scholar
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed. Sci. & Technol. 48: 185–197.10.1016/0377-8401(94)90171-6CrossRefGoogle Scholar
Cone, J.W. (1994). A new automated gas production method for the in vitro study of fermentation kinetics in rumen fluid using pressure transducers and electic gas valves. Proc. Soc. Nutr. Physiol. 3:182 Google Scholar