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Total phenols and protein precipitation assays as indicators of the inhibitory effects of phenols on rumen micro-organisms

Published online by Cambridge University Press:  21 November 2017

C D Wood  and
V E Plumb
C D Wood
Affiliation:
Natural Resources Institute, Central Avenue Chatham Maritime Kent ME4 4TB
V E Plumb
Affiliation:
Natural Resources Institute, Central Avenue Chatham Maritime Kent ME4 4TB
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Extract

Phenolics, such as tannins, have been implicated as inhibitors of digestibility. A wide range of assays for measuring phenols have been proposed (reviewed by Hagerman and Butler, 1989) but there is little experimental evidence to indicate which are most appropriate for assessing nutritive value. The objective of this study was to investigate correlations between phenol assays and the effects of phenolics on in vitro fermentation by rumen micro-organisms to identify which assays most reliably indicated the extent of these effects.

Extracts were prepared from 12 Bolivian tree leaf samples using 70% aqueous acetone (500mg dried sample extracted with 5 ml solvent) and analysed for total phenols (TP) by the Prussian Blue method (Price and Butler, 1977), protein precipitation activity (PPA) by a radial diffusion technique (Hagerman, 1987) and condensed tannins (CT) by acid butanol (Porter et al, 1986). The samples used are listed in Table 1.

Type
Digestion and Rumen metabolism
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1994 , March 1994 , pp. 75
Copyright
Copyright © The British Society of Animal Production 1994

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References

Hagerman, A E 1987 Radial diffusion method for determining tannin in plant extracts Journal of Chemical Ecology 13: 437–449 Google Scholar
Hagerman, A E and Butler, L G 1989 Choosing appropriate methods and standards for assaying tannins Journal of Chemical Ecology 15: 1795 –1810 Google Scholar
Merry, R J, Theodorou, , Merry, R J, Theodorou, M K, Raurich, M G, Dhanoa, M S 1991 Use of head-space gas pressure in batch cultures to assist in determination of nutritive value of silage to rumen bacteria Proceedings of a conference on forage conservation towards 2000, held January 1991, Institute of Grassland and Forage Research, Federal Research Centre of Agriculture, Braunschweig-Volkenrode Google Scholar
Porter, L J, Hrstich, L N and Chan, B G 1986 The conversion of procyanidins and prodelphinidins to cyanidins and delphinidin Phytochemistry 25: 223–230 Google Scholar