Published online by Cambridge University Press: 21 November 2017
The rumen in situ technique has been adopted as the standard method (AFRC, 1992) for the characterisation of nitrogen in feedstuffs for ruminants. Differences in the degradation characteristics of identical samples both within and between laboratories have been observed (Madsen and Hvelplund, 1985; Oldham, 1987; Kandylis and Nikokyris, 1991). These variations may relate to a number of variables including, cloth type, weave, pore size, sample preparation, basal diet of host and post-incubation washing procedure. Another of the possible causes of variation between similar measurements may be dynamic changes in rumen metabolism. Wilson and Briggs (1955) observed that the day-to-day variation in viable bacterial counts in animals fed a fixed diet was even greater than those observed with gross changes in basal diet. The aim of the present experiment was to observe the day-to-day variation in the rumen of sheep fed a constant diet over 18 weeks.