Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-28T04:49:55.394Z Has data issue: false hasContentIssue false
  • English
  • Français

Validation of QTLs associated with spikelets per panicle and grain weight in rice

Published online by Cambridge University Press:  16 July 2014

Sang-Min Yeo ,
Yeo-Tae Yun ,
Dong-Min Kim ,
Chong-Tae Chung  and
Sang-Nag Ahn
Sang-Min Yeo
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
Yeo-Tae Yun
Affiliation:
Chungnam Agricultural Research and Extension Services, Yesan 340-861, Republic of Korea
Dong-Min Kim
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
Chong-Tae Chung
Affiliation:
Chungnam Agricultural Research and Extension Services, Yesan 340-861, Republic of Korea
Sang-Nag Ahn*
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
*
* Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study, a near-isogenic line (BC4F10) CR572 developed by introgressing a chromosomal segment from Oryza rufipogon (accession no. 105491) into the Oryza sativa subsp. japonica cv. Hwaseong was found to exhibit a significant increase in the number of spikelets per panicle (SPP) and grain weight compared with the recurrent parent Hwaseong. Quantitative trait locus (QTL) analysis in F2 generation derived from the cross between CR572 and Hwaseong revealed that two QTLs, qSPP1 and qTGW1, were linked to a simple sequence repeat marker, RM283, on chromosome 1. The additive effect of the O. rufipogon allele at qSPP1 was 13 SPP, and 21.6% of the phenotypic variance was explained by the segregation of RM283. The qTGW1 QTL explained 19.1% of the phenotypic variance for grain weight. Substitution mapping was carried out with five F3 lines derived from F2 plants having informative recombination breakpoints within the target region. Substitution mapping indicated the linkage of qSPP1 and qTGW1. The grain yield of CR572 was 18.2 and 15.8% higher than that of Hwaseong at two locations, respectively, mainly due to the increase in 1000-grain weight and SPP. These results are very useful for QTL cluster transfer by molecular marker-assisted selection in rice breeding programmes and for QTL gene cloning by map-based cloning.

Keywords

1000-grain weightQTLnear-isogenic linesricespikelets per panicle
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S151 - S154
Copyright
Copyright © NIAB 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ashikari, M, Sakakibara, H, Lin, SY, Yamamoto, T, Takashi, T, Nishimura, A, Angeles, ER, Qian, Q, Kitano, H and Matsuoka, M (2005) Cytokinin oxidase regulates rice grain production. Science 309: 741–745.Google Scholar
Causse, MA, Fulton, TM, Cho, YG, Ahn, SN, Chunwogse, J, Wu, K, Xiao, J, Yu, Z, Ronald, PC, Harrington, SE, Second, G, McCouch, SR and Tanksley, SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251–1374.Google Scholar
Cho, YC, Suh, JP, Choi, IS, Hong, HC, Baek, MK, Kang, KH, Kim, YG, Ahn, SN, Choi, HC, Hwang, HG and Moon, HP (2003) QTLs analysis of yield and its related traits in wild rice relative Oryza rufipogon . Treatises of Crop Research 4: 19–29.Google Scholar
Fan, C, Xing, Y, Mao, H, Lu, T, Han, B, Xu, C, Li, X and Zhang, Q (2006) GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theoretical Applied Genetics 112: 1164–1171.Google Scholar
Ji, HS, Chu, SH, Jiang, W, Chi, YI, Hahn, JH, Eun, MY, McCouch, SR and Koh, HJ (2006) Characterization and mapping of a shattering mutant in rice that corresponds to a block of domestication genes. Genetics 173: 995–1005.Google Scholar
Li, Y, Fan, C, Xing, Y, Jiang, Y, Luo, L, Sun, L, Shao, D, Xu, C, Li, X, Xiao, J, He, Y and Zhang, Q (2011) Natural variation in GS5 plays an important role in regulating grain size and yield in rice. Nature Genetics 43: 1266–1269.CrossRefGoogle ScholarPubMed
Liu, T, Shao, D, Kovi, MR and Xing, Y (2010) Mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (Oryza sativa L.). Theoretical Applied Genetics 120: 933–942.Google Scholar
Shomura, A, Izawa, T, Ebana, K, Ebitani, T, Kanegae, H, Konishi, S and Yano, M (2008) Deletion in a gene associated with grain size increased yields during rice domestication. Nature Genetics 40: 1023–1028.Google Scholar
Song, XJ, Huang, W, Shi, M, Zhu, MZ and Lin, HX (2007) A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nature Genetics 39: 623–630.Google Scholar
Suh, J, Ahn, SN, Cho, YC, Kang, KH, Choi, IS, Kim, YG, Suh, HS and Hwang, HG (2005) Mapping for QTLs for yield traits using an advanced backcross population from a cross between Oryza sativa and O. glaberrima . Korean Journal Breeding Science 37: 214–220.Google Scholar
Thomson, MJ, Tai, TH, McClung, AM, Lai, XH, Hinga, ME and Lobos, KB (2003) Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theoretical Applied Genetics 107: 479–493.Google Scholar
Xie, XB, Jin, FX, Song, MH, Suh, JP, Hwang, HG, Kim, YG, McCouch, SR and Ahn, SN (2008) Fine mapping of a yield-enhancing QTL cluster associated with transgressive variation in an Oryza sativa× O. rufipogon cross. Theoretical Applied Genetics 116: 613–622.CrossRefGoogle Scholar
Yun, YT, Kim, DM, Park, IK, Chung, CT, Seong, YK and Ahn, SN (2010) Development and evaluation of QTL-NILs for grain weight from an interspecific cross in rice. Korean Journal Breeding Science 42: 357–364.Google Scholar
Zhang, YS, Luo, LJ, Xu, CG, Zhang, QF and Xing, YZ (2006) Quantitative trait loci for panicle size, heading date and plant height co-segregating in trait-performance derived near-isogenic lines of rice (Oryza sativa). Theoretical Applied Genetics 113: 361–368.Google Scholar