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The use of simple sequence repeat (SSR) markers to identify and map alien segments carrying genes for effective resistance to leaf rust in bread wheat

Published online by Cambridge University Press:  01 August 2007

Nayyer Iqbal ,
Firdissa Eticha ,
Elena K. Khlestkina ,
Annette Weidner ,
Marion S. Röder  and
Andreas Börner
Nayyer Iqbal
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Firdissa Eticha
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Elena K. Khlestkina
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Annette Weidner
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Marion S. Röder
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
Andreas Börner*
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany
*
*Corresponding author. E-mail: [email protected]
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Abstract

Aegilops markgrafii is a useful source of genes encoding both resistance to biotic stress and high seed lysine content. Bread wheat/Ae. markgrafii introgression lines expressing leaf rust resistance were developed from a cross between a leaf rust-resistant Ae.markgrafii accession and the susceptible bread wheat cultivar ‘Alcedo’. The content of introgressed segments present in five sister introgression lines was assessed with the help of chromosome-specific simple sequence repeats (SSRs). One of the lines was used as a parent of a 140 individual F2 mapping population, by crossing with the leaf rust-susceptible bread wheat cv. ‘Borenos’. The population was tested for susceptibility or resistance to leaf rust, and linkage analysis indicated the presence of a quantitative trait locus (QLr.ipk-2A) originating from the Ae. markgrafii parent, mapping to the distal segment of chromosome arm 2AS.

Keywords

Aegilops markgrafiidisease resistanceinterspecific introgression linesmicrosatellite markersPuccinia reconditaTriticum aestivum
Type
In Brief
Information
Plant Genetic Resources , Volume 5 , Issue 2 , August 2007 , pp. 100 - 103
Copyright
Copyright © NIAB 2007

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References

Cenci, A, D'Ovidio, R, Tanzarella, OA, Ceoloni, C and Porceddu, E (1999) Identification of molecular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theoretical and Applied Genetics 98: 448–454.CrossRefGoogle Scholar
Endo, TR (1983) Gametocidal chromosomes of three Aegilops species in common wheat. Canadian Journal of Genetics and Cytology 24: 201–206.CrossRefGoogle Scholar
Endo, TR (1988) Chromosome mutations induced by gametocidal chromosomes in common wheat. In: Miller, TE and Koebner, RMD (eds) Proceedings of the 7th International Wheat Genetics Symposium. Bath, Avon, UK: Bath Press, pp. 259–265.Google Scholar
Endo, TR (1996) Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous group 2. Genes and Genetic Systems 71: 243–246.CrossRefGoogle Scholar
Endo, TR and Katayama, Y (1978) Finding of a selectively retained chromosome of Aegilops caudata L. in common wheat. Wheat Information Service 47: 32–35.Google Scholar
Frauenstein, K and Hammer, K (1985) Testing Aegilops species for their reaction to mildew, Erysiphe graminis DC., brown rust, Puccinia recondita Rob. Ex Desm., and glume blotch, Septoria nodorum Berk. Kulturpflanze 33: 155–163.CrossRefGoogle Scholar
Friebe, B, Badaeva, ED, Hammer, K and Gill, BS (1996) Standard karyotypes of Aegilops uniaristata. Ae. mutica, Ae. comosa subspecies comosa and heldreichii (Poaceae). Plant Systematics and Evolution 202: 199–210.CrossRefGoogle Scholar
Iqbal, N, Miller, TE, Reader, SM and Calligari, PDS (2000) Characterization of Aegilops uniaristata chromosomes by comparative DNA marker analysis and repetitive DNA sequence in situ hybridization. Theoretical and Applied Genetics 101: 1173–1179.CrossRefGoogle Scholar
Leonova, I, Börner, A, Budashkina, E, Kalinina, N, Unger, O, Röder, M and Salina, E (2004) Identification of microsatellite markers for a leaf rust resistance gene introgressed into common wheat from Triticum timopheevii. Plant Breeding 123: 93–95.CrossRefGoogle Scholar
McIntosh, RA, Wellings, CR and Park, RF (1995) Wheat Rusts: An Atlas of Resistance Genes. Dordrecht: CSIRO/Kluwer Academic Publishers, p. 10.Google Scholar
McIntosh, RA, Yamazaki, Y, Devos, KM, Dubcovsky, J, Rogers, J and Appels, R (2003) Catalogue of Gene Symbols, http://www.grs.nig.ac.jp/wheat/komugi/genes/.Google Scholar
Peil, A, Korzun, V, Schubert, V, Schumann, E, Weber, WE and Röder, MS (1998) The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines. Theoretical and Applied Genetics 96: 138–146.CrossRefGoogle Scholar
Röder, MS, Korzun, V, Wendehake, K, Plaschke, J, Tixier, MH, Leroy, P and Ganal, MW (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.CrossRefGoogle ScholarPubMed
Schachermayr, GM, Messmer, MM, Feuillet, C, Winzeler, H, Winzeler, M and Keller, B (1995) Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theoretical and Applied Genetics 90: 982–990.CrossRefGoogle Scholar
Schubert, V (2001) Die Sammlung und Charakterisierung pflanzlicher genetischer Ressourcen und ihre Nutzung im Rahmen der Züchtungsforschung bei Weizen. Dissertationes Botanicae. Vol. 352. Berlin: J. Cramer Verlagsbuchhandlung.Google Scholar
Schubert, V, Junghanns, W, Weidner, A, Oertel, C and Blüthner, WD (1995) Powdery mildew and leaf rust resistance from Aegilops markgrafii. In Börner A and Worland AJ (eds) Proceedings of the 9 th European Wheat Aneuploids Co-operative Conference, Gatersleben-Wernigerode, 1994. Germany: IPK Gatersleben, pp 77–79.Google Scholar
Somers, DJ, Isaac, P and Edwards, K (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109: 1105–1114.CrossRefGoogle ScholarPubMed
Valkoun, J, Hammer, K, Kucerova, D and Bartoš, P (1985) Disease resistance in the genus Aegilops L.—stem rust, leaf rust, stripe rust, and powdery mildew. Kulturpflanze 33: 133–153.CrossRefGoogle Scholar
Weidner, A (2004) Selektion und Charakterisierung braunrostresistenter Weizen—Aegilops markgrafii—Introgressionslinien. PhD Thesis, Martin-Luther-University Halle-Wittenberg.Google Scholar
Xie, C, Sun, Q, Ni, Z, Yang, T, Nevo, E and Fahima, T (2003) Chromosomal location of Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theoretical and Applied Genetics 106: 341–345.CrossRefGoogle ScholarPubMed