Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-14T19:20:35.215Z Has data issue: false hasContentIssue false

A rapid simple sequence repeat (SSR)-based identification method for potato cultivars

Published online by Cambridge University Press:  01 April 2007

A. Reid*
Affiliation:
Scottish Agricultural Science Agency, 1 Roddinglaw Road, Edinburgh, EH12 9FJ, UK
E. M. Kerr
Affiliation:
Scottish Agricultural Science Agency, 1 Roddinglaw Road, Edinburgh, EH12 9FJ, UK
*
*Corresponding author. E-mail: [email protected]

Abstract

With the numbers of new varieties of potato obtaining Plant Breeders' Rights increasing yearly, the reliable maintenance of large culture collections is becoming more problematic. Additionally, the differentiation of cultivars based on morphological characteristics is a highly skilled and time-consuming task and for these reasons a rapid and robust method for variety differentiation has become highly desirable. By screening a number of microsatellite (simple sequence repeat, SSR) markers we have developed a set of six that can be used to differentiate over 400 cultivars, including those on the UK National List, but excluding somaclonal variants (e.g. Red King Edward and King Edward). The whole process from tuber to accurate identification can be carried out in a single day.

Type
Research Article
Copyright
Copyright © Crown Copyright 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anon. (2003a) Potatoes get their chips, www.food.gov.uk/newsarchive/potatoes.Google Scholar
Anon. (2003b) Survey to investigate the varietal labelling of potatoes—part 1,www.food.gov.uk/multimedia/pdfs/fsis4403.pdf.Google Scholar
Anon. (2005) Final report into the 2003 potato ring rot outbreak in the UK,www.defra.gov.uk/planth/ring/repfinal.htm.Google Scholar
Bornet, B, Goraguer, D, Joly, G and Branchard, M (2002) Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeat (ISSRs). Genome 45: 481484.CrossRefGoogle Scholar
Brownstein, MJ, Carpten, JD and Smith, JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. BioTechniques 20: 10041010.Google Scholar
Cooke, RJ (1999) New approaches to potato variety identification. Potato Research 42: 529539.Google Scholar
Coombs, JJ, Frank, LM and Douches, DS (2004) An applied fingerprinting system for cultivated potato using simple sequence repeats. American Journal of Potato Research 81: 243250.Google Scholar
Corbett, G, Lee, D, Donini, P and Cooke, RJ (2001) Identification of potato varieties by DNA profiling. Acta Horticulturae 546: 387390.Google Scholar
Davey, R (2004) Time to end this damaging practice. Eyewitness (British Potato Council), p. 15.Google Scholar
Demeke, T, Kawchuk, LM and Lynch, DR (1993) Identification of potato cultivars and clonal variants by random amplified polymorphic DNA analysis. American Potato Journal 70: 561570.CrossRefGoogle Scholar
Ghislain, M, Spooner, DM, Rodríguez, F, Villamón, F, Núñez, J, Vásquez, C, Waugh, R and Bonierbale, M (2004) Selection of highly informative and user-friendly microsatellites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics 108: 881890.CrossRefGoogle Scholar
Görg, R, Schachtschabel, U, Ritter, E, Salamini, F and Gebhardt, T (1992) Discrimination among 136 tetraploid potato varieties by fingerprints using highly polymorphic DNA markers. Crop Science 32: 815–819.Google Scholar
Hosaka, K, Mori, M and Ogawa, K (1994) Genetic relationships of Japanese potato cultivars assessed by RAPD analysis. American Potato Journal 71: 535–546.Google Scholar
Houwing, A, Suk, R and Ros, B (1986) Generation of lightsprouts suitable for potato variety identification by means of artificial light. Acta Horticulturae 182: 359–363.Google Scholar
Isenegger, DA, Taylor, PWJ, Ford, R, Franz, P, McGregor, GR and Hutchinson, JF (2001) DNA fingerprinting and genetic relationships of potato cultivars (Solanum tuberosum L.) commercially grown in Australia. Australian Journal of Agricultural Research 52: 911918.Google Scholar
Kawchuk, LM, Lynch, DR, Thomas, J, Penner, B, Sillito, D and Kulcsar, F (1996) Characterization of Solanum tuberosum simple sequence repeats and application to potato cultivar identification. American Potato Journal 73: 325–335.Google Scholar
Kim, JH, Juong, H, Kim, HY and Lim, YP (1998) Estimation of genetic variation and relationship in potato (Solanum cultivars L.) cultivars using AFLP markers. American Journal of Potato Research 75: 107–112.Google Scholar
McGregor, CE, Lambert, CA, Greyling, MM, Louw, JH and Warnich, L (2000) A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135144.Google Scholar
Milbourne, D, Meyer, R, Bradshaw, JE, Baird, E, Bonar, N, Provan, J, Powell, W and Waugh, R (1997) Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular Breeding 3: 127136.Google Scholar
Milbourne, D, Meyer, RC, Collins, AJ, Ramsey, LD, Gebhardt, C and Waugh, R (1998) Isolation, characterisation and mapping of simple sequence repeat loci in potato. Molecular and General Genetics 259: 233245.Google Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Science USA 70: 33213323.Google Scholar
Norero, N, Malleville, J, Huarte, M and Feingold, S (2002) Cost efficient poato (Solanum tuberosum L.) cultivar identification by microsatellite amplification. Potato Research 45: 131–138.Google Scholar
Provan, J, Powell, W and Waugh, R (1996) Microsatellite analysis of relationships within cultivated potato (Solanum tuberosum). Theoretical and Applied Genetics 92: 10781084.CrossRefGoogle Scholar
Scheider, K and Douches, DS (1997) Assessment of PCR-based simple sequence repeats to fingerprint North American potato cultivars. American Potato Journal 74: 149–160.Google Scholar
Sosinski, B and Douches, DS (1996) Using polymerase chain reaction-based DNA amplification to fingerprint North American potato cultivars. Horticultural Science 31: 130–133.Google Scholar