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Polymorphism in Gloriosa superba

Published online by Cambridge University Press:  01 April 2009

Seemanti Ghosh ,
Biswajit Ghosh  and
Sumita Jha
Seemanti Ghosh
Affiliation:
Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata700019, India
Biswajit Ghosh
Affiliation:
Department of Botany, RKMVC College, 24 Parganas (North), Rahara, West Bengal, India
Sumita Jha*
Affiliation:
Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata700019, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Gloriosa superba L. is a highly valued indigenous medicinal plant. It is available throughout India with noticeable variations in morphology and colchicine content. In the present study, morphological, phytochemical, cytological and molecular data are employed to screen the polymorphism, if present, in five different populations of G. superba L. Based on gross morphological traits, the plants can be grouped into two clusters: Amtala (AM) and Baruipur (BR) plants belonging to one cluster, and Siliguri (SG), Darjeeling (DJ) and Sikkim (SK) plants belonging to the other. Colchicine content of the tubers ranged from 0.06% in AM plants to 0.37% in BR plants. Cytological analysis revealed that the SK plants showed 44 chromosomes, i.e. tetraploid status, whereas the other four were diploid. Assessment of the karyomorphology of the four diploid populations indicated a close similarity between AM and BR plants, whereas the SG plants exhibited slightly different karyomorphology and lacked sm-type chromosomes. Random Amplified Polymorphic DNA (RAPD) analysis revealed 76% polymorphism among the populations. Cluster analysis using RAPD resulted in three main cluster groups; BR and SG plants clustered together indicating that they are most closely related when compared with other populations.

Keywords

colchicinediversitykaryomorphologymarkersRAPD
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 1 , April 2009 , pp. 9 - 15
Copyright
Copyright © NIAB 2008

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References

Anonymous (1956) Gloriosa superba. In: The Wealth of India (Raw Materials) IV. New Delhi: CSIR, p. 139.Google Scholar
Aros, D, Meneses, C and Infante, R (2006) Genetic diversity of wild species and cultivated varieties of alstroemeria estimated through morphological descriptors and RAPD markers. Scientia Horticulturae 108: 86–90.CrossRefGoogle Scholar
Brown, AHD (1979) Enzyme polymorphism in plant population. Theoretical Population Biology 15: 1–42.CrossRefGoogle Scholar
Cabrita, LF, Alsoy, U, Hepaksoy, S and Leitao, JM (2001) Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Scientia Horticulturae 87: 261–263.CrossRefGoogle Scholar
Carr, GD, King, RM, Powell, AM and Robinson, H (1999) Chromosome numbers in Compositae XVIII. American Journal of Botany 86: 1003–1013.CrossRefGoogle ScholarPubMed
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Google Scholar
Forrest, I, Burg, K and Klump, R (2000) Genetic markers: tools for identifying and characterizing Scots pine populations. Investigacion Agraria, Sistemas y Recursos Forestales: Fuera de Serie n 1-2000: 67–88.Google Scholar
Garcia-Mas, J, Oliver, M, Gomez-Paniagua, H and de Vicente, MC (2000) Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theoretical Applied Genetics 101: 860–864.CrossRefGoogle Scholar
Ghosh, B, Mukherjee, M, Jha, TB and Jha, S (2002) Enhanced colchicine production in root cultures of Gloriosa superba by direct and indirect precursors of the biosynthetic pathway. Biotechnology Letters 24: 231–234.CrossRefGoogle Scholar
Hamrick, JL (1983) The distribution of genetic variation within and among natural plant populations. In: Schonewald-Cox, CM, Chambers, SM, MacBryde, B and Thomas, WL (eds) Genetics and Conservation. London: Benjamin/Cummings, pp. 335–344.Google Scholar
Hayashi, T, Yoshida, K and Sano, K (1988) Formation of alkaloids in suspension-cultured Colchicum autumnale. Phytochemistry 27: 1371–1374.CrossRefGoogle Scholar
Ho, KY, Yang, JC and Hsiao, JY (2002) An assessment of genetic diversity and documentation of hybridization of casuarinas grown in Taiwan using RAPD markers. International Journal of Plant Sciences 163: 831–835.CrossRefGoogle Scholar
Insel, PA (1996) Analgesic antipyretic and anti-inflammatory agents and drugs employed in the treatment of gout: colchicine. In: Harman, JG, Limbrid, LE and Molinoff, RW (eds) Goodman and Gilman's The Pharmacological Basis of Therapeutics. 9th ed.New York, NY: McGraw-Hill, pp. 647–649.Google Scholar
Iqbal, MJ, Aziz, N, Saeed, NA, Zafar, Y and Malik, KA (1997) Genetic diversity evaluation of some elite cotton varieties by RAPD analysis. Theoretical Applied Genetics 94: 139–144.CrossRefGoogle ScholarPubMed
Lashermes, P, Cross, J, Marmey, P and Charrier, A (1993) Use of random amplified DNA markers to analyse genetic variability and relationships of Coffea species. Genetic Resources and Crops Evolution 44: 91–99.CrossRefGoogle Scholar
Levan, A, Fredga, K and Sandberg, AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.CrossRefGoogle Scholar
Linhart, YB, Mitton, JB, Sturgeon, KB and Davis, ML (1981) Genetic variation in space and time in a population of ponderosa pine. Heredity 46: 407–426.CrossRefGoogle Scholar
Mahmood, S and Abbas, A (2003) Local population differentiation in Trifolium alexandrium L. in response to various disturbance regimes. Online Journal of Biological Sciences 3(9): 773–781.Google Scholar
Martin, C, Juliano, A, Newbury, HJ, Lu, B-R, Jackson, MT and Ford-Lloyd, BV (1997) The use of RAPD marker to facilitate the identification of Oryza species within a germplasm collection. Genetic Resources and Crops Evolution 44: 175–183.CrossRefGoogle Scholar
Ran, Y, Murray, BG and Hammett, KRW (2001) Evaluating genetic relationships between and within Clivia species using RAPDs. Scientia Horticulturae 90: 167–179.CrossRefGoogle Scholar
Seegmiller, JE, Howell, RR and Malawista, SE (1962) A mechanism of action of colchicine in acute gouty arthritis. Journal of Clinical Investigation 41: 1399.Google Scholar
Sokal, RR and Rohlf, FJ (1987) Introduction to Biostatistics. New York: WH Freeman and Company, pp. 178–179.Google Scholar
Williams, JGK, Kubelik, AR, Livak, KJ, Rfalski, JA and Tingey, SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.CrossRefGoogle ScholarPubMed