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Phenotypic variants among ethyl methanesulfonate M2 mutant lines in Capsicum annuum

Published online by Cambridge University Press:  16 July 2014

Doyeon Hwang ,
Hee-Jin Jeong ,
Jin-Kyung Kwon ,
Hohyun Kim ,
Si-Yong Kang  and
Byoung-Cheorl Kang
Doyeon Hwang
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Hee-Jin Jeong
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Jin-Kyung Kwon
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Hohyun Kim
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Si-Yong Kang
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Byoung-Cheorl Kang*
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
*
* Corresponding author. E-mail: [email protected]
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Abstract

Induction of mutations using chemical mutagens has proved to be a useful tool in crop improvement and has advantages over transgenic approaches in view of legislative restrictions and intellectual property. Among the chemical mutagens, ethyl methanesulfonate (EMS) has been widely used to generate novel traits. In this study, we constructed an EMS mutant population consisting of 3945 M2 mutant lines using a Korean landrace of Capsicum annuum ‘Yuwol-cho’. In total, 1480 M2 mutant lines were evaluated for novel traits. The mutant lines showed phenotypic variations such as plant growth (small size and dwarfism), development of leaves (variegation, colour and morphological changes), flowers (inflorescence, morphological and organ colour changes), and fruits (morphological and colour changes). Most of these mutant phenotypes were inherited recessively. Many of the mutant phenotypes were unique in pepper, while some were similar to those of known mutants in other plant species. These mutant lines will be useful for the study of gene function in C. annuum.

Keywords

ethyl methanesulfonatemutant linesnovel traitspepper
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S141 - S145
Copyright
Copyright © NIAB 2014 

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References

Alcantara, TP, Bosland, PW and Smith, DW (1996) Ethyl methanesulfonate-induced seed mutagenesis of Capsicum annuum . Journal of Heredity 87: 239–241.CrossRefGoogle Scholar
Bosland, PW (1992) Chiles: a diverse crop. HortTechnology 2: 6–10.CrossRefGoogle Scholar
Bosland, PW (2002) Inheritance of a novel flaccid mutant in Capsicum annuum . Journal of Heredity 93: 380–382.CrossRefGoogle ScholarPubMed
Bosland, PW and Votava, E (2012) Peppers: Vegetable and Spice Capsicums, 2nd edn. UK: CAB International, Cambridge, 230pp.CrossRefGoogle Scholar
Daskalov, S (1973) Investigation of induced mutants in Capsicum annuum L. III. Mutants in the variety Zlaten Medal. Genetics and Plant Breeding 6: 419–429.Google Scholar
Daskalov, S (1974) Investigation on induced mutants in sweet pepper (Capsicum annuum L.). In Proceedings of 1st Meeting of the Capsicum Breeding and Genetics, 1–4 July 1974, Budapest, Hungary, pp. 81–90.Google Scholar
Elitzur, T, Nahum, H, Borovsky, Y, Pekker, I, Eshed, Y and Paran, I (2009) Co-ordinated regulation of flowering time, plant architecture and growth by FASCICULATE: the pepper orthologue of SELF PRUNING . Journal of Experimental Botany 60: 869–880.CrossRefGoogle ScholarPubMed
Jeong, HJ, Kwon, JK, Pandeya, D, Hwang, J, Hoang, NH, Bae, JH and Kang, BC (2011) A survey of natural and ethyl methane sulfonate-induced variations of eIF4E using high-resolution melting analysis in Capsicum . Molecular Breeding 29: 349–360.CrossRefGoogle Scholar
Lippert, LF, Bergh, BO and Cook, AA (1964) Three variegated seedling mutants in the pepper. Journal of Heredity 55: 78–93.CrossRefGoogle Scholar
Menda, N, Semel, Y, Peled, D, Eshed, Y and Zamir, D (2004) In silico screening of a saturated mutation library of tomato. Plant Journal 38: 861–872.CrossRefGoogle ScholarPubMed
Minoia, S, Petrozza, A, D'Onofrio, O, Piron, F and Mosca, G (2010) A new mutant genetic resource for tomato crop improvement by TILLING technology. BMC Research Notes 3: 69.CrossRefGoogle ScholarPubMed
Muth, J, Hartje, S, Twyman, RM, Hofferbert, H-R, Tacke, E and Prüfer, D (2008) Precision breeding for novel starch variants in potato. Plant Biotechnology Journal 6: 576–584.CrossRefGoogle ScholarPubMed
Okabe, Y, Asamizu, E, Saito, T, Matsukura, C, Ariizumi, T, Brès, C, Rothan, C, Mizoguchi, T and Ezura, H (2011) Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries. Plant Cell Physiology 52: 1994–2005.CrossRefGoogle ScholarPubMed
Paran, I, Borovsky, Y, Nahon, S and Cohen, O (2007) The use of induced mutations to study shoot architecture in Capsicum . Israel Journal of Plant Science 55: 125–131.CrossRefGoogle Scholar
Parry, MA, Madgwick, PJ, Bayon, C, Tearall, K, Hernandez-Lopez, A, Baudo, M, Rakszegi, M, Hamada, W, Al-Yassin, A, Ouabbou, H, Labhilili, M and Phillips, AL (2009) Mutation discovery for crop improvement. Journal of Experimental Botany 60: 2817–2825.CrossRefGoogle ScholarPubMed
Tadmor, Y, Katzir, N, Meir, A, Yaniv-Yaakov, A, Sa'ar, U, Baumkoler, F, Lavee, T, Lewinsohn, E, Schaffer, A and Buerger, J (2007) Induced mutagenesis to augment the natural genetic variability of melon (Cucumis melo L.). Israel Journal of Plant Sciences 55: 159–169.CrossRefGoogle Scholar
Triques, K, Sturbois, B, Gallais, S, Dalmais, M, Chauvin, S, Clepet, C, Aubourg, S, Rameau, C, Caboche, M and Bendahmane, A (2007) Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea. Plant Journal 51: 1116–1125.CrossRefGoogle ScholarPubMed
Van der Beek, JG and Ltifi, A (1990) Variation in fasciculation in F4 populations of pepper. Capsicum Eggplant Newsletter 8/9: 34–35.Google Scholar
Wang, D and Bosland, PW (2006) The genes of Capsicum . HortScience 41: 1169–1187.CrossRefGoogle Scholar
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