Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-28T06:41:17.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization of the Glu-Ay gene from Triticum urartu for its potential use in quality wheat breeding

Published online by Cambridge University Press:  30 March 2011

M. V. Gutiérrez ,
C. Guzmán ,
L. M. Martín  and
J. B. Alvarez
M. V. Gutiérrez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
C. Guzmán
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
L. M. Martín
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
J. B. Alvarez*
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Triticum urartu Thum. ex Gandil. is a wild species identified as A-genome donor for polyploid wheats, which could be used as gene source for wheat breeding. The high-molecular weight glutenin subunits are endosperm storage proteins that are associated with bread-making quality. In T. urartu, these proteins are encoded by the Ax and Ay genes at the Glu-Au1 locus. The Ay gene of 17 Glu-Au1 allelic variants previously detected in this species has been analysed using PCR amplification and digestion of the PCR products with two endonucleases (DdeI and PstI). The combination of two restriction patterns has revealed variations between the active and inactive alleles, and within each type. This variation, especially that detected among the active alleles, could enlarge the high-quality genetic pool of modern wheat and be used for bread-making quality improvement in durum and common wheat.

Keywords

electrophoresisgenetic resourcesgluteninquality breedingwild wheat
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 334 - 337
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alvarez, JB, D'Ovidio, R and Lafiandra, D (1998) Analysis of x- and y-type genes presente at the Glu-A1 encoding high molecular weight glutenin subunits in wild and cultivated wheats. Proceedings of 9th Wheat Genetics Symposium, vol. 4. Saskatoon: University Extension Press, University of Saskatchewan, pp. 127–129.Google Scholar
Alvarez, JB, Caballero, L, Nadal, S, Ramírez, MC and Martín, A (2009) Development and gluten strength evaluation of introgression lines of Triticum urartu in durum wheat. Cereal Research Communications 37: 243–248.CrossRefGoogle Scholar
Caballero, L, Martín, MA and Alvarez, JB (2008) Allelic variation for the high-and-low-molecular-weight glutenin subunits in wild diploid wheat (Triticum urartu) and its comparison with durum wheats. Australian Journal of Agricultural Research 59: 906–910.CrossRefGoogle Scholar
Ciaffi, M, Lafiandra, D, Porceddu, E and Benedettelli, S (1993) Storage-protein variation in wild emmer wheat (Triticum turgidum ssp. dicoccoides) form Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theoretical and Applied Genetics 86: 474–480.CrossRefGoogle Scholar
Ciaffi, M, Lafiandra, D, Turchetta, T, Ravaglia, S, Bariana, H, Gupta, RB and MacRitchie, F (1995) Bread-baking potential of durum wheat lines expressing both x-and y-type subunits at the Glu-A1 locus. Cereal Chemistry 72: 465–469.Google Scholar
Cornish, GB, Békés, F, Eagles, HA and Payne, PI (2006) Prediction of dough properties for bread wheats. In: Wrigley, C, Békés, F and Bushuk, W (eds) Gliadin and Glutenin: The Unique Balance of Wheat Quality. St. Paul, MN: AACC International Press, pp. 243–280.CrossRefGoogle Scholar
D'Ovidio, R, Masci, S and Porceddu, E (1995) Development of a set of oligonucleotide primers specific for genes at the Glu-1 complex loci of wheat. Theoretical and Applied Genetics 91: 189–194.CrossRefGoogle ScholarPubMed
Dvorak, J, Terlizzi, P, Zhang, HB and Resta, P (1993) The evolution of polyploidy wheats: identification of the A genome species. Genome 36: 21–31.CrossRefGoogle Scholar
Godfray, HCJ, Beddington, JR, Crute, IR, Haddad, L, Lawrence, D, Muir, JF, Pretty, J, Robinson, S, Thomas, SM and Toulmin, C (2010) Food security: the challenge of feeding 9 billion people. Science 327: 812–818.CrossRefGoogle ScholarPubMed
Hajjar, R and Hodgkin, T (2007) The use of wild relative in crop improvement: a survey of developments over the last 20 years. Euphytica 156: 1–13.CrossRefGoogle Scholar
Harberd, NP, Bartels, D and Thompson, RD (1986) DNA restriction fragment variation in the gene family encoding high-molecular-weight (HMW) glutenin subunits of wheat. Biochemical Genetics 24: 579–596.CrossRefGoogle ScholarPubMed
Lafiandra, D, Tucci, GF, Pavoni, A, Turchetta, T and Margiotta, B (1997) PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theoretical and Applied Genetics 94: 235–240.CrossRefGoogle Scholar
Payne, PI (1987) Genetics of wheat storage proteins and the effects of allelic variation on bread-making quality. Annual Review of Plant Physiology 38: 141–153.CrossRefGoogle Scholar
Stacey, J and Isaac, P (1994) Isolation of DNA from plants. In: Isaac, PG (ed.) Methods in Molecular Biology: Protocols for Nucleic Acid Analysis by Non-Radiactive Probes. Totawa: Humana Press, pp. 9–15.Google Scholar
Waines, JG and Payne, PI (1987) Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum). Theoretical and Applied Genetics 74: 71–76.CrossRefGoogle ScholarPubMed