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Mining simple sequence repeats in Picrorhiza kurroa transcriptomes for assessing genetic diversity among accessions varying for picrosides contents

Published online by Cambridge University Press:  28 September 2015

Kirti Shitiz ,
Saurabh Pandit ,
Sreekrishna Chanumolu ,
Hemant Sood ,
Harvinder Singh ,
Jagdish Singh  and
Rajinder Singh Chauhan
Kirti Shitiz
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
Saurabh Pandit
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
Sreekrishna Chanumolu
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
Hemant Sood
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
Harvinder Singh
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
Jagdish Singh
Affiliation:
Agroforestry and Extension Division, Himalayan Forest Research Institute, Panthaghati-171009, Shimla, Himachal Pradesh, India
Rajinder Singh Chauhan*
Affiliation:
Department of Biotechnology and Bioinformatics, Jaypee University, Waknaghat-173234, Solan, Himachal Pradesh, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Picrorhiza kurroa (family Scrophulariaceae) is a high value medicinal herb used in herbal drug formulations like picroliv, picrolax, etc. It has been collected recklessly from its natural habitat posing endangered status for its existence, thereby necessitating characterization of genetic diversity for its sustainable utilization and conservation. In this study, picrosides content and genetic profiles of 26 accessions of P. kurroa from different locations in north-western Himalayas have been analysed. Picroside-I (P-I) content ranged from 0.37 to 2.7% in fresh shoots whereas total picrosides content (P-I+P-II) ranged from 3.7 to 10.9% in dry rhizomes. High picrosides content accession PKS-1 was identified, both for shoots and rhizomes. To study genetic diversity and correlate picrosides content with their genetic factors, genetic profiling was done using simple sequence repeats (SSRs) identified from P. kurroa transcriptomes. Out of 361 SSR primers tested on 26 accessions, 35 primers yielded polymorphic profiles. Overall low genetic diversity was observed in P. kurroa accessions. The highest polymorphism information content (PIC) of 0.55 was given by SSR marker PKSTS-P9 (TGGTG)4. Mean allele number was 2.97. Mean observed and expected heterozygosities were 0.597 and 0.452, respectively. Among all accessions Nei's genetic diversity (H) was 0.39 and Shannon's information index (I) was 0.58. Cluster analysis of STRUCTURE was comparable to DARWIN. Microsatellite markers would be helpful in the development of DNA diagnostics for the authentication of quality plant material as well as planning a genetic improvement strategy.

Keywords

genetic diversityHPLCmarkersPicrorhiza kurroapicrosidesSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 1 , February 2017 , pp. 79 - 88
Copyright
Copyright © NIAB 2015 

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