Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-14T15:18:22.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of high-oleic peanut chemical mutants and functional analysis of mutated FAD2B gene

Published online by Cambridge University Press:  04 May 2022

Hong Wei Han ,
Zhi Wei Wang ,
Xiu Zhen Wang ,
Xiu Shan Sun ,
Chao Qi Fang  and
Chuan Tang Wang Open the ORCID record for Chuan Tang Wang [Opens in a new window]
Hong Wei Han
Affiliation:
Shenyang Agricultural University, Shenyang 110866, China
Zhi Wei Wang
Affiliation:
Shandong Peanut Research Institute, Qingdao 266100, China
Xiu Zhen Wang
Affiliation:
Shandong Peanut Research Institute, Qingdao 266100, China
Xiu Shan Sun
Affiliation:
Shandong Peanut Research Institute, Qingdao 266100, China
Chao Qi Fang
Affiliation:
Yicheng District Agricultural & Rural Bureau, Zaozhuang 277300, China
Chuan Tang Wang*
Affiliation:
Shenyang Agricultural University, Shenyang 110866, China Shandong Peanut Research Institute, Qingdao 266100, China Shandong Luhua Group Company, Laiyang 265200, China
*
Author for correspondence: Chuan Tang Wang, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

It is generally accepted that high-oleic crops have at least 70% oleate. As compared to their normal-oleic counterparts, oil and food products made from high-oleic peanut have better keeping quality and are much healthier. Therefore, high-oleic peanut is well recognized by processors and consumers. However, owing to the limited availability of high-oleic donors, most present-day high-oleic peanut varietal releases merely have F435 type FAD2 mutations. Through screening of a mutagenized peanut population of 15L46, a high-yielding peanut line with desirable elliptical oblong large seeds, using near infrared model for predicting oleate content in individual single seeds, high-oleic peanut mutants were identified. Sequencing FAD2A and FAD2B of the mutants along with the wild type revealed that these mutants possessed G448A FAD2A (F435 type FAD2A mutation) and G558A FAD2B (non-F435 type FAD2B mutation). Expression of the wild and mutated type FAD2B in yeast verified that the functional mutation contributed to the high-oleic phenotype in these mutants. The mutants provided additional high-oleic donors to peanut quality improvement.

Keywords

Fatty acid desaturaseG558A mutationhigh oleategroundnutyeast expression
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 15 - 21
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Equally contributed authors.

References

Anonymous (2021) Sort peanut. SEEDMEISTER Mark IIIx Tests & Sorts Peanuts for Oleic & Linoleic Acid. Brimrose. Available at https://static1.squarespace.com/static/58cd62b759cc688ab46997dd/t/5e8df0e35a06753ee4256b89/1586360548715/SEEDMEISTER-MARKIIIx-solution-001-1.pdf (Accessed September 12 2021).Google Scholar
Barbour, JA, Howe, PRC, Buckley, JD, Bryan, J and Coates, AM (2017) Cerebrovascular and cognitive benefits of high-oleic peanut consumption in healthy overweight middle-aged adults. Nutritional Neuroscience 20, 555–562.CrossRefGoogle ScholarPubMed
Bimro, ET, Hovav, R, Nyska, A, Glazer, TA and Madar, Z (2020) High oleic peanuts improve parameters leading to fatty liver development and change the microbiota in mice intestine. Food & Nutrition Research 64, 4278.CrossRefGoogle ScholarPubMed
Cao, XS, Cao, GX, Wu, JJ, Zhan, Y, Ding, B, Li, M and Xie, XD (2020) Construction and application of yeast high-efficient expression vector pYES2-T. Genomics and Applied Biology 39, 2607–2613.Google Scholar
Chen, SL, Cheng, ZS, Wang, J, Song, YH, Liu, YJ, Zhang, PJ and Li, YR (2018) Identification and breeding application of a new high-oleic peanut mutant. In Proceedings of the 8th Conference of Oil Crop Professional Committee of Chinese Crop Society. Qingdao, p. 214.Google Scholar
Committee of Health and Planning (CHP), China and Food and Drug Supervision and Administration of China (FDSAC) (2017) Determination of fatty acids in foods. GB 5009.168‒2016. Beijing, China: China Standards Press.Google Scholar
Davis, BI, Agraz, CB, Kline, M, Gottschall, E, Nolt, M, Whitaker, TB, Osborne, JA, Tengstrand, E, Ostrowski, K, Teixeira, R and Davis, JP (2021) Measurements of high oleic purity in peanut lots using rapid, single kernel near-infrared reflectance spectroscopy. Journal of the American Oil Chemists’ Society 98, 621–632.CrossRefGoogle Scholar
Fang, CQ, Wang, CT, Wang, PW, Tang, YY, Wang, XZ, Feng, GC and Yu, ST (2012) Identification of a novel mutation in FAD2B from a peanut EMS mutant with elevated oleate content. Journal of Oleo Science 61, 143–148.CrossRefGoogle ScholarPubMed
Jung, S, Powell, G, Moore, K and Abbott, A (2000 a) The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. II. Molecular basis and genetics of the trait. Molecular & General Genetics 263, 806–811.CrossRefGoogle ScholarPubMed
Jung, S, Swift, D, Sengoku, E, Patel, M, Teulé, F, Powell, G, Moore, K and Abbott, A (2000 b) The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. I. Isolation and characterization of two genes encoding microsomal oleoyl-pc desaturases. Molecular & General Genetics 263, 796–805.CrossRefGoogle ScholarPubMed
Knauft, DA, Moore, KM and Gorbet, DW (1993) Further studies on the inheritance of fatty acid composition in peanut. Peanut Science 20, 74–76.CrossRefGoogle Scholar
López, Y, Nadaf, HL, Smith, OD, Connell, JP, Reddy, AS and Fritz, AK (2000) Isolation and characterization of the Δ12-fatty acid desaturase in peanut (Arachis hypogaea L.) and search for polymorphisms for the high oleate trait in Spanish market-type lines. Theoretical & Applied Genetics 101, 1131–1138.CrossRefGoogle Scholar
López, Y, Nadaf, HL, Smith, OD, Simpson, CE and Fritz, AK (2002) Expressed variants of Δ12-fatty acid desaturase for the high oleate trait in Spanish market-type peanut lines. Molecular Breeding 9, 183–192.CrossRefGoogle Scholar
Moore, KM and Knauft, DA (1989) The inheritance of high-oleic acid in peanut. Journal of Heredity 80, 252–253.CrossRefGoogle Scholar
Nada, FHL, Biradar, K, Murthy, G, Krishnaraj, PU, Bhat, RS, Pasha, MA and Yerimani, AS (2017) Novel mutations in oleoyl-pc desaturase, identified from new high oleic mutants induced by gamma rays in peanut. Crop Science 57, 2538–2546.CrossRefGoogle Scholar
Nawade, B, Mishra, GP and Radhakrishnan, T (2018) High oleic peanut breeding: achievements perspectives and prospects. Trends in Food Science & Technology 78, 107–119.CrossRefGoogle Scholar
Nkuna, RT, Wang, CT, Wang, XZ, Tang, YY and Zhang, JC (2021) Sodium azide induced high-oleic peanut (Arachis hypogaea L.) mutant of Virginia type. Genetic Resources and Crop Evolution 68, 1759–1767.CrossRefGoogle Scholar
Norden, AJ, Borget, DW, Knauft, DA and Young, CT (1987) Variability in oil quality among peanut genotypes in the Florida breeding program. Peanut Science 4, 7–11.CrossRefGoogle Scholar
O'Connor, DJ, Meder, R, Furtado, A, Henry, R, Wright, GC and Rachaputi, RCN (2021) Single kernel sorting of high and normal oleic acid peanuts using near infrared spectroscopy. Journal of Near Infrared Spectroscopy 29, 366–370.CrossRefGoogle Scholar
Patel, M, Jung, S, Moore, K, Powell, G, Ainsworth, C and Abbott, A (2004) High-oleate peanut mutants result from a MITE insertion into the FAD2 gene. Theoretical & Applied Genetics 108, 1492–1502.CrossRefGoogle ScholarPubMed
Ray, TK, Holly, SP, Knauft, DA, Abbott, AG and Powell, GL (1993) The primary defect in developing seed from the high oleate variety of peanut (Arachis hypogaea L.) is the absence of Δ12-desaturase activity. Plant Science 91, 15–21.CrossRefGoogle Scholar
Wang, CT and Zhu, LG (ed.) (2017) High Oleic Acid Peanut. Shanghai, China: Shanghai Science and Technology Press.Google Scholar
Wang, CT, Wang, XZ, Tang, YY, Wu, Q, Xu, JZ, Hu, DQ and Qu, B (2014 a) Predicting main fatty acids, oil and protein content in intact single seeds of groundnut by near infrared reflectance spectroscopy. Advanced Materials Research 860–863, 490–496.CrossRefGoogle Scholar
Wang, T, Hu, DQ, Wang, M, Chen, MN, Pan, LJ, Chi, XY and Yu, SL (2014 b) Present situation and development trend of peanut consumption in the world. Journal of Peanut Science 43, 39–45.Google Scholar
Wang, ML, Tonnis, B, An, YQC, Pinnow, D, Tishchenko, V and Pederson, GA (2015) Newly identified natural high-oleate mutant from Arachis hypogaea L. subsp. hypogaea. Molecular Breeding 35, 186.CrossRefGoogle Scholar
Xu, Y and Zhang, SL (2011) Research progress on high oleic acid peanut breeding in China. Guangdong Agricultural Science 38, 43–45.Google Scholar
Yu, SL, Pan, LJ, Yang, QL, Min, P, Ren, ZK and Zhang, HS (2000) Comparison of the Δ12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. Journal of Genetics and Genomics 35, 679–685.CrossRefGoogle Scholar
Yu, ST, Wang, CT, Wang, H, Yu, GQ, Shi, PX, Ren, L, Yu, HB and Du, ZB (2019) Two-step chemical mutagen treatment to convert Fuhua 12, a normal oleic Spanish peanut cultivar, into its high oleic version. IOP Conference Series. Earth and Environmental Science 346, 012039.CrossRefGoogle Scholar
Zhao, Z, Shi, A, Wang, Q and Zhou, J (2019) High oleic acid peanut oil and extra virgin olive oil supplementation attenuate metabolic syndrome in rats by modulating the gut microbiota. Nutrients 11, 3005.CrossRefGoogle ScholarPubMed
Supplementary material: Image

Han et al. supplementary material

Han et al. supplementary material 1

Download Han et al. supplementary material(Image)
Image 10.7 MB
Supplementary material: Image

Han et al. supplementary material

Han et al. supplementary material 2

Download Han et al. supplementary material(Image)
Image 3.2 MB
Supplementary material: File

Han et al. supplementary material

Han et al. supplementary material 3

Download Han et al. supplementary material(File)
File 13.8 KB