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Genetic relationships among Prunus rootstocks for sweet cherry (Prunus avium L.) cultivars

Published online by Cambridge University Press:  27 April 2012

Z. Turkoglu ,
A. Koc ,
S. Ercisli ,
S. Bilgener ,
M. Akbulut ,
N. Yildirim ,
R. Gercekcioglu ,
A. Esitken  and
M. Gunes
Z. Turkoglu
Affiliation:
Ministry of Food, Agriculture and Livestock, Atatürk Orman Çiftliği, Ankara, Turkey
A. Koc
Affiliation:
Black Sea Agricultural Research Institute, Gelemen, Samsun, Turkey
S. Ercisli*
Affiliation:
Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
S. Bilgener
Affiliation:
Department of Horticulture, Faculty of Agriculture, Ondokuz Mayis University, Samsun, Turkey
M. Akbulut
Affiliation:
Rize University, Pazar Vocational School, Rize, Turkey
N. Yildirim
Affiliation:
Ankara University Biotechnology Institute, Ankara, Turkey
R. Gercekcioglu
Affiliation:
Department of Horticulture, Faculty of Agriculture, Gaziosmanpasa University, Tokat, Turkey
A. Esitken
Affiliation:
Department of Horticulture, Faculty of Agriculture, Selcuk University, Konya, Turkey
M. Gunes
Affiliation:
Department of Horticulture, Faculty of Agriculture, Gaziosmanpasa University, Tokat, Turkey
*
*Corresponding author. E-mail: [email protected]
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Abstract

Sweet cherries can be grafted on a wide range of rootstocks belonging to Prunus avium, Prunus cerasus, Prunus mahaleb, Prunus angustifolia or hybrids of different Prunus species. Identification of Prunus rootstocks using morphological traits is almost impossible particularly during the dormant season. However, molecular analysis carried out on actively growing shoot tips, leaves or dormant buds provides good opportunity to reliably distinguish rootstocks. In this study, DNA was extracted from the leaves of a total of 184 sweet cherry rootstock candidates belonging to P. avium L., P. cerasus L., P. mahaleb L. and P. angustifolia L. previously selected from the north-western part of Turkey. The rootstock candidates were tested with ten simple sequence repeat (SSR) primers, developed for the Prunus genus. The primers successfully identified all rootstock candidates. The results showed that the number of alleles per locus ranged from 10 (UDAp-401, UCD-CH21 and CPSCT010) to 20 (UCD-CH31) with an average of 13.3 alleles per locus, indicating that the SSRs were highly informative. Unweighted Pair-Group Method with Arithmetic mean analysis demonstrated that P. avium accessions are closely related to P. cerasus. The reference rootstocks were clustered with their associated botanical species.

Keywords

genetic diversitygenotypingPrunusSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 10 , Issue 2 , August 2012 , pp. 101 - 107
Copyright
Copyright © NIAB 2012

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