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Genetic diversity of Juglans sigillata Dode germplasm in Yunnan Province, China, as revealed by SSRs

Published online by Cambridge University Press:  17 November 2020

Shaoyu Chen Open the ORCID record for Shaoyu Chen [Opens in a new window] ,
Tao Wu ,
Liangjun Xiao ,
Delu Ning  and
Li Pan
Shaoyu Chen*
Affiliation:
Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry and Grassland, Kunming650204, Yunnan, China
Tao Wu
Affiliation:
Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry and Grassland, Kunming650204, Yunnan, China
Liangjun Xiao
Affiliation:
Economic Forest Institute, Yunnan Academy of Forestry and Grassland, Kunming650204, Yunnan, China
Delu Ning
Affiliation:
Economic Forest Institute, Yunnan Academy of Forestry and Grassland, Kunming650204, Yunnan, China
Li Pan
Affiliation:
Economic Forest Institute, Yunnan Academy of Forestry and Grassland, Kunming650204, Yunnan, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Iron walnut, Juglans sigillata Dode, restricted to southwestern China, has its centre of distribution in Yunnan Province which has a varied climate, geography and rich plant diversity. Yunnan contains abundant J. sigillata germplasm. In this study, a provincial-scale set of walnut germplasm resources (14 populations comprising 1122 individuals) was evaluated for genetic diversity based on 20 simple sequence repeat (SSR) loci. The number of SSR alleles per locus ranged from 7 to 27, with an average of 17.55. Mean allelic richness and mean private allelic richness ranged from 3.40 to 4.62 and 0.11 to 0.36, with average of 3.93 and 0.30, respectively. Expected heterozygosity (He) varied from 0.26 to 0.78, with an average of 0.57. Polymorphism information content ranged from 0.22 to 0.79, with an average of 0.57. Genetic differentiation (FST) was 0.05, indicating that only 5% of total genetic variability was inter-populational, a finding supported by an analysis of molecular variance and STRUCTURE analysis. Relatively high gene flow (Nm = 6.70) was observed between populations. A unweighted pair-group method with arithmetic analysis classified the 14 populations into two major groups. Mantel testing uncovered a significant correlation between geographic distance and genetic distance (r = 0.33, P = 0.04). Overall, the research revealed a moderately high level of genetic diversity in the germplasm and low genetic differentiation among populations, which showed great potential for further development and exploitation of this resource.

Keywords

genetic differentiationgenetic diversityJuglans sigillata DodeSSR markersUPGMA clustering
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 6 , December 2020 , pp. 417 - 426
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

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