Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-20T16:37:50.162Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic diversity in woad (Isatis tinctoria L.) accessions detected by ISSR markers

Published online by Cambridge University Press:  16 March 2011

Luís Rocha ,
Sandra Martins ,
Valdemar Carnide ,
Fernando Braga  and
Carlos Carvalho
Luís Rocha
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Sandra Martins
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Valdemar Carnide
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Fernando Braga
Affiliation:
Centre of Chemistry of Vila Real, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Carlos Carvalho*
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Woad (Isatis tinctoria L.) was introduced in Europe in ancient times to produce indigo, a natural blue pigment used mainly for dyestuff. This species was cultivated in Portugal until the beginning of the 20th century, especially in the inner North and South. A set of nine inter-simple sequence repeat (ISSR) markers generated 177 reproducible fragments, of which 171 were polymorphic. The mean number of fragments/accession was 111, ranging between 100 (Portugal-Coimbra) and 124 (Poland). The total polymorphism observed was 0.3272, the average polymorphism was 0.1784 and the gene differentiation between accessions was 0.4546. Polymorphism ranged between 53.8% (Austria) and 73.1% (Belgium). The genetic relationship among woad accessions was obtained with unweighted pair group method with arithmetic mean dendrogram based on a molecular marker, clearly clustering the woad accessions according to their geographic origin. The genetic diversity observed in this collection shows that there is a considerable potential for its improvement and that ISSR could be used to evaluate intra- and inter-accession similarities in I. tinctoria species.

Keywords

genetic relationshipsinter-simple sequence repeat-PCR markersIsatis tinctoria L.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 210 - 213
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Al-Qurainy, F (2010) Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.). African Journal of Biotechnology 9: 467–474.Google Scholar
Andersen, NS, Poulsen, G, Andersen, BA, Kiær, LP, D'Hertefeldt, T, Wilkinson, MJ and Jørgensen, RB (2009) Processes affecting genetic structure and conservation: a case study of wild and cultivatedc Brassica rapa. Genetic Resources and Crop Evolution 56: 189–200.CrossRefGoogle Scholar
Darlington, CD and Wyle, AP (1955) Chromosome Atlas of Flowering Plants. London: George Allen & Unwin Ltd, p. 38.Google Scholar
Gilbert, KG and Cooke, DT (2001) Dyes from plants: past usage, present understanding and potential. Plant Growth Regulation 34: 57–69.CrossRefGoogle Scholar
Gilbert, KG, Garton, S, Karam, MA, Arnold, GM, Karp, A, Edwards, KJ, Cooke, DT and Barker, JHA (2002) A high degree of genetic diversity is revealed in Isatis spp. (dyer's woad) by amplified fragment length polymorphism (AFLP). Theoretical and Applied Genetics 104: 1150–1156.CrossRefGoogle Scholar
Lu, X, Liu, L, Gong, Y, Zhao, L, Song, X and Zhu, X (2009) Cultivar identification and genetic diversity analysis of broccoli and its related species with RAPD and ISSR markers. Scientia Horticulturae 122: 645–648.CrossRefGoogle Scholar
Martín, JP and Sánchez-Yélamo, MD (2000) Genetic relationships among species of the genus Diplotaxis (Brassicaceae) using inter-simple sequence repeat markers. Theoretical and Applied Genetics 101: 1234–1241.CrossRefGoogle Scholar
Oberthur, C, Graf, H and Hamburger, M (2004) The content of indigo precursors in Isatis tinctoria leaves – a comparative study of selected accessions and post-harvest treatments. Phytochemistry 65: 3261–3268.CrossRefGoogle Scholar
Pasqualone, A, Caponio, F and Blanco, A (2001) Inter-simple sequence repeat DNA markers for identification of drupas from different Olea europaea L. cultivars. European Food Research and Technology 213: 240–243.CrossRefGoogle Scholar
Paterson, AH, Tanksley, SD and Sorrells, ME (1991) DNA markers in plant improvement. Advances in Agronomy 46: 39–90.CrossRefGoogle Scholar
Rakoczy-Trojanowska, M and Bolibok, H (2004) Characteristics and a comparison of three classes of microsatellite-based markers and their application in plants. Cellular and Molecular Biology Letters 9: 221–238.Google Scholar
Spataro, G and Negri, V (2008) Adaptability and variation in Isatis tinctoria L.: a new crop for Europe. Euphytica 163: 89–102.CrossRefGoogle Scholar
Spataro, G, Taviani, P and Negri, V (2007) Genetic variation and population structure in a Eurasian collection of Isatis tinctoria L. Genetic Resources and Crop Evolution 54: 573–584.CrossRefGoogle Scholar
Stoker, KG, Cooke, DT and Hill, DJ (1998) Influence of light on natural indigo production from woad (Isatis tinctoria). Plant Growth Regulation 25: 181–185.CrossRefGoogle Scholar
Zietkiewicz, E, Rafalski, A and Labuda, D (1994) Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction. Genomics 20: 176–183.CrossRefGoogle ScholarPubMed