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Genetic diversity in the USDA Limnanthes germplasm collection assessed by simple sequence repeats

Published online by Cambridge University Press:  01 April 2009

Linda M. Donnelly ,
Maria M. Jenderek ,
James P. Prince ,
Patrick A. Reeves ,
Allan Brown  and
Richard M. Hannan
Linda M. Donnelly
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA Department of Biology, California State University Fresno, Fresno, CA 93740, USA
Maria M. Jenderek*
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA
James P. Prince
Affiliation:
Department of Biology, California State University Fresno, Fresno, CA 93740, USA
Patrick A. Reeves
Affiliation:
National Center for Genetic Resources Preservation, USDA-ARS, Fort Collins, CO 80521, USA
Allan Brown
Affiliation:
National Arid Land Plant Genetic Research Unit, USDA-ARS, Parlier, CA 93648, USA
Richard M. Hannan
Affiliation:
Western Regional Plant Introduction Station, USDA-ARS, Pullman, WA 99164, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

The genus Limnanthes (Limnanthaceae), also known as meadowfoam, has attracted attention for industrial use due to the unique characteristics of its seed oil. Samples from wild populations showed variability in agronomically important traits involved in seed oil yield, warranting the establishment and continued development of a germplasm collection. The level of genetic diversity within the United States Department of Agriculture, Agriculture Research Service, National Plant Germplasm System (USDA-ARS-NPGS) Limnanthes collection was evaluated using 15 simple sequence repeat (SSR) markers across 62 accessions representing 7 species. Parsimony analysis separated the accessions into two main groups consistent with the traditional taxonomic sections Inflexae and Reflexae, but there was little resolution within groups. These two groups were confirmed using neighbour-joining analysis and principal coordinate analysis. SSR marker variation suggests that the Limnanthes germplasm collection is genetically diverse and the accessions within the species likely contain novel alleles, and therefore the collection contributes to the conservation of the wild Limnanthes gene pool.

Keywords

conservation geneticsgenetic diversitygermplasm resourcesmeadowfoamsimple sequence repeat
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 1 , April 2009 , pp. 33 - 41
Copyright
Copyright © NIAB 2008

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