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Genetic diversity in Anatolian wild grapes (Vitis vinifera subsp. sylvestris) estimated by SSR markers

Published online by Cambridge University Press:  26 January 2011

Ali Ergül ,
Gemma Perez-Rivera ,
Gökhan Söylemezoğlu ,
Kemal Kazan  and
Rosa Arroyo-Garcia
Ali Ergül
Affiliation:
Biotechnology Institute, Ankara University, Ankara, Turkey
Gemma Perez-Rivera
Affiliation:
Centro de Biotecnología y Genómica de Plantas (CBGP-INIA), Campus de Montegancedo, Autovía M40 Km38, Pozuelo de Alarcón 28223Madrid, Spain
Gökhan Söylemezoğlu
Affiliation:
Department of Horticulture, Faculty of Agriculture, Ankara University, Ankara, Turkey
Kemal Kazan
Affiliation:
Commonwealth Scientific and Industrial Research Organization (CSIRO) Plant Industry, Queensland Bioscience Precinct, St. Lucia, Queensland, QLD 4067, Australia
Rosa Arroyo-Garcia*
Affiliation:
Centro de Biotecnología y Genómica de Plantas (CBGP-INIA), Campus de Montegancedo, Autovía M40 Km38, Pozuelo de Alarcón 28223Madrid, Spain
*
*Corresponding author. E-mail: [email protected]
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Abstract

Anatolia (Asia Minor) is considered to be the cradle of viticulture, but wild grape accessions from this region have not been subjected to any genetic analysis. We present the first genetic characterization of wild grapes (Vitis vinifera subsp. sylvestris) from this region. Using 15 nuclear microsatellites, we genotyped 84 wild grape accessions collected from three Anatolian locations. The unweighted pair group method with arithmetic mean analysis revealed four genetic clusters that partially separated Anatolian wild grape populations. In general, accessions from geographically closer locations showed higher genetic similarities than those from more distant locations. In some cases, accessions from one population showed close genetic relationships to accessions from a different population. The genetic diversity between natural populations from both ends of the Mediterranean basin has shown higher genetic diversity in the Anatolian Peninsula than peripheral populations, suggesting that this area could be one centre of diversity of the species. The genetic relationship between wild and cultivated grapevine from Anatolian Peninsula indicated a clear separation between them. However, we cannot discarded a local genetic contribution. Overall, this study reveals useful information for management and potential utilization of Anatolian wild grape germplasm.

Keywords

Anatolian Peninsulacultivated grapevinegenetic diversitygenetic relationshipIberian Peninsulawild grapevine
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 3 , August 2011 , pp. 375 - 383
Copyright
Copyright © NIAB 2011

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References

Andres, MT, Benito, A, Perez-Rivera, G, Ocete, R, Lopez, MA, Gaforio, L, Muñoz, G, Cabello, F, Martinez-Zapater, JM and Arroyo-Garcia, R. Genetic diversity of wild grapevine populations in the Iberian Peninsula and their genetic relationship with cultivated grapevine (submitted).Google Scholar
Arroyo-García, R, Ruiz-García, L, Bolling, L, Ocete, R, Lopez, MA, Arnold, C, Ergul, A, Söylemezoglu, G, Uzun, HI, Cabello, F, Ibáñez, J, Aradhya, MK, Atanassov, A, Atanassov, I, Balint, S, Cenis, JL, Costantini, L, Goris-Lavets, S, Grando, MS, Klein, BY, McGovern, PE, Merdinoglu, D, Pejic, I, Pelsy, F, Primikirios, N, Risovannaya, V, Roubelakis-Angelakis, KA, Snoussi, H, Sotiri, P, Tamhankar, S, This, P, Troshin, L, Malpica, JM, Lefort, F and Martinez-Zapater, JM (2006) Múltiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Molecular Ecology 15: 3707–3714.CrossRefGoogle ScholarPubMed
Belaj, A, Muñoz-Diez, C, Baldoni, L, Procedí, A, Barranco, D and Satovic, Z (2007) Genetic diversity and populations structure of wild olives from the North-western Mediterranean assessed by SSR markers. Annals of Botany 100: 449–458.CrossRefGoogle ScholarPubMed
Bowcock, A, Ruíz-Linares, A, Tomfohrde, J, Minch, E, Kidd, JR and Cavalli-Sforza, LL (1994) High resolution of human evolutionary trees with polymorphic microsatellites. Nature (London) 368: 455–457.Google Scholar
Bowers, JE, Dangl, GS and Meredith, CP (1999) Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture 50: 243–246.Google Scholar
Bowers, JE, Dangl, GS, Vignani, R and Meredith, CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L). Genome 39: 628–633.CrossRefGoogle ScholarPubMed
Cunha, J, Balerias-Couto, M, Cunha, JP, Banza, J, Soveral, A, Carneiro, LC and Eiras-Dias, JE (2007) Characterization of Portuguese populations of Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi. Genetic Resources and Crop Evolution 54: 981–988.Google Scholar
De Andres, MT, Cabezas, JA, Ververa, MT, Borrego, J, Martinez-Zapater, JM and Jouve, N (2007) Molecular characterization of grapevine rootstocks maintained in germplasm collections. American Journal of Enology and Viticulture 58: 75–86.Google Scholar
Di Vechhi-Staraz, M, Laucou, V, Bruno, G, Lacombe, T, Gerber, S, Bourse, T, Boselli, M and This, P (2009) Low level of pollen mediated gene flow from cultivated to wild grapevine: consequences for the evolution of the endangered subspecies Vitis vinifera L. subsp silvestris. Journal of Heredity 100: 66–75.CrossRefGoogle Scholar
Ergül, A, Marasalı, B and Ağaoğlu, YS (2002) Molecular discrimination and identification of some Turkish grape cultivars (Vitis vinifera L.) by RAPD markers. Vitis 41: 159–160.Google Scholar
Ergül, A, Kazan, K, Aras, S, Çevik, V, Çelik, H and Söylemezoğlu, G (2006) AFLP analysis of genetic variation within the two economically important Anatolian grapevine (Vitis vinifera L.) varietal groups. Genome 49: 467–495.Google Scholar
Excoffier, L, Smouse, PE and Quattro, JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.Google Scholar
Felsenstein, J (1989) Phylogeny inference package. Cladistics 5: 164–166.Google Scholar
Gök Tangolar, S, Soydam, S, Bakır, M, Karaağaç, E, Tangolar, S and Ergül, A (2009) Genetic analysis of grapevine cultivars from the eastern Mediterranean region of Turkey, based on SSR Markers. Tarım Bilimleri Dergisi 15: 1–8.Google Scholar
Goudet, J (2001) FSTAT: a program to estimate and test gene diversities and fixation (version 2.9.3). Available at http://www2.sunil.ch/popgen/softwares/fstat.htm.Google Scholar
Grassi, F, Labra, M, Imazio, S, Spada, A, Sgorbati, S, Scienza, A and Sala, F (2003) Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theoretical and Applied Genetics 107: 1315–1320.CrossRefGoogle ScholarPubMed
Heywood, V and Zohary, D (1991) A catalogue of wild relatives of cultivated plants native to Europe. Flora Mediterranea 5: 375–415.Google Scholar
Karataş, H, Değirmenci, D, Velasco, R, Vezzulli, S, Bodur, Ç and Ağaoğlu, YS (2007) Microsatellite fingerprinting of homonymous grapevine (Vitis vinifera L.) varieties in neighboring regions of South-East Turkey. Sciencia Horticulturae 114: 164–169.CrossRefGoogle Scholar
Lopes, MS, Mendoça, D, Rodrigues do Santos, JE, Eiras-Dias, JE and da Camara Machado, A (2009) New insights on the genetic basis of Portuguese grapevine and on grapevine domestication. Genome 52: 790–800.CrossRefGoogle ScholarPubMed
McGovern, PE (2004) Ancient Wine: The Search for the Origins of Viticulture. Princeton, NJ: Princeton University Press.Google Scholar
Merdinoglu, D, Butterlin, G, Bevilacqua, L, Chiquet, V, Adam-Blondon, AF and Decroocq, S (2005) Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR. Molecular Breeding 15: 349–366.CrossRefGoogle Scholar
Minch, E, Ruíz-Linares, A, Goldstein, D, Feldman, M, Kidd, JR and Cavalli-Sforza, LL (1997) Microsat 1.5: A Computer Program for Calculating Various Statistics on Microsatellite Data. Pullman, WA: Washington State University.Google Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70: 3321–3323.Google Scholar
Page, RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–360.Google Scholar
Peakall, R and Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology 6: 288–295.CrossRefGoogle Scholar
Riahi, L, Soghlami, N, El-Heir, K, Laucou, V, Cunff, LL, Boursiquot, JM, Lacombe, T, Mliki, A, Ghorbel, A and This, P (2010) Genetic structure and differentiation among grapevines (Vitis vinifera L.) accessions from Maghred region. Genetic Resources and Crop Evolution 57: 255–272.Google Scholar
Schuster, WSF and Mitton, JB (2000) Paternity and gene dispersal in limber pine (Pinus flexilis James). Heredity 84: 348–361.Google Scholar
Şelli, F, Bakır, M, İnan, G, Aygün, H, Boz, Y, Yaşasın, AS, Özer, C, Akman, B, Söylemezoğlu, G, Kazan, K and Ergül, A (2007) Simple sequence repeat-based assessment of genetic diversity in Dimrit and Gemre grapevine accessions from Turkey. Vitis 46: 182–187.Google Scholar
Streiff, R, Ducousso, A, Lexer, C, Steinkellner, H, Gloessl, J and Kremer, A (1999) Pollen dispersal inferred from paternity analisis in a mixed oak stand of Quercus robur L. and Q. petrea (Matt.) Liebl. Molecular Ecology 8: 831–841.CrossRefGoogle Scholar
This, P, Lacombe, T and Thomas, MR (2006) Historical origins and genetic diversity of wine grapes. Trends in Genetics 22: 511–519.CrossRefGoogle ScholarPubMed
Thomas, MR and Scott, NS (1993) Microsatellites repeats in grapevine reveal DNA polymorphism when analysed as sequence tagged sites (STSs). Theoretical and Applied Genetics 86: 985–990.Google Scholar
Turpeinen, T, Tehola, T, Manninen, O, Nevo, E and Nissila, E (2001) Microsatellite diversity associated with ecological factors in Hordeum spontaneum populations in Israel. Molecular Ecology 10: 1577–1591.Google Scholar
Weir, BS and Cookerman, C (1984) Estimating F-statistics for the analysis of population genetics. Evolution 38: 1358–1370.Google Scholar
Yüksel, C. (2008) SSRs based genetic characterization of Manisa, İzmir, Aydın, Muğla and Kütahya grapevine germplasms, Dissertation, University of Ankara.Google Scholar
Zinelabidine, LH, Haddioui, A, Bravo, G, Arroyo-Garcia, R and Martinez-Zapater, JM (2010) Genetic origins of cultivated and wild grapevines from Morocco. American Journal of Enology and Viticulture 61: 1.CrossRefGoogle Scholar
Zohary, D and Hopf, M (2000) Domestication of Plants in the Old Word: The Origin and Spread of Cultivated Plants in West Asia, Europe and Nile Valley. 3rd edn. New York: Oxford University.Google Scholar
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