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Genetic diversity and structure of the threatened anti-cancerous plant Nothapodytes nimmoniana as revealed by ISSR analysis

Published online by Cambridge University Press:  22 July 2011

V. K. Abdul Kareem ,
P. E. Rajasekharan ,
S. Mini  and
T. Vasantha Kumar
V. K. Abdul Kareem
Affiliation:
Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore560 089, India
P. E. Rajasekharan*
Affiliation:
Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore560 089, India
S. Mini
Affiliation:
Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore560 089, India
T. Vasantha Kumar
Affiliation:
Section of Medicinal Crops, Indian Institute of Horticultural Research, Hessaraghatta Lake, Bangalore560 089, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Inter simple sequence repeat markers were used to assess the genetic diversity and population genetic structure in 12 populations of Nothapodytes nimmoniana from Western Ghats of India. A total of 16 selected primers produced 103 discernible bands, with 76 (73.7%) being polymorphic. The Nei's gene diversity (h) ranged from 0.1166 to 0.2124, with an average of 0.1518 at the population level and 0.2965 at the species level indicating high genetic diversity. The Shannon's index (I) was estimated to be 0.2189 within populations (range 0.1703–0.2947) and 0.4352 at the species level. The analysis of molecular variance showed that the genetic variation was found mainly within populations (73%), but variance among populations was only 27% and its value, ΦPT = 0.271, P < 0.001, implied that high genetic differentiation among populations. In addition, Nei's differentiation coefficient (GST) was found to be high (0.4882) and the gene flow (Nm) was low (0.5242), confirming the high population genetic differentiation. The unweighted pair-group method using arithmetic average clustering elicited similar results. Based on this, we propose conservation strategy for this plant species.

Keywords

anti-cancercamptothecingenetic differentiationgenetic diversityISSRNothapodytes nimmonianapopulation structureWestern Ghats
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 4 , December 2011 , pp. 506 - 514
Copyright
Copyright © NIAB 2011

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References

Ayala, FJ and Kiger, JA (1984) Modern Genetics. 2nd edn. Menlo Park, CA: Benjamin/Cummings.Google Scholar
Barrett, SCH (1992) Genetics of weed invasions. In: Jain, SK and Botsford, LW (eds) Applied Population Biology. Dordrecht: Kluwer Academic Publishers, pp. 91–119.Google Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Google Scholar
Ellstrand, NC and Elam, DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annual Review of Ecology and Systematics 24: 217–242.CrossRefGoogle Scholar
Excoffier, L, Smouse, PE and Quattro, JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction data. Genetics 131: 479–491.CrossRefGoogle ScholarPubMed
Fang, DQ, Roose, ML, Krueger, RR and Federici, CT (1997) Fingerprinting trifoliate orange germplasm accessions with isozymes, RFLPs and inter-simple sequence repeat markers. Theoretical and Applied Genetics 95: 211–219.Google Scholar
Francisco-Ortega, J, Santos-Guerra, A, Kim, SC and Crawford, DJ (2000) Plant genetic diversity in the Canary Islands: a conservation perspective. American Journal of Botany 87: 909–919.CrossRefGoogle ScholarPubMed
Frankham, R, Ballou, JD and Briscoe, DA (2002) Introduction to Conservation Genetics. Cambridge: Cambridge University Press, p. 617.CrossRefGoogle Scholar
Govindachari, TR and Viswanathan, N (1972) Alkaloids of Mappia foetida. Phytochemistry 11: 3529–3531.Google Scholar
Hamrick, JL and Godt, MJW (1989) Allozyme diversity in plant species. In: Brown, AHD, Clegg, MT, Kahler, AL and Weir, BS (eds) Plant Population Genetics, Breeding and Genetic Resources. Sunderland, MA: Sinauer Associates, pp. 43–63.Google Scholar
Hijmans, RJ, Guarino, L, Jarvis, A, O'Brien, R, Mathur, P, Bussink, C, Cruz, M, Barrantes, I and Rojas, E (2005) DIVA-GIS version 5.2. manual Available at http://www.diva-gis.Google Scholar
Holl, KD and Hayes, GF (2006) Challenges to introducing and managing disturbance regimes for Holocarpha macradenia, an endangered annual grassland forb. Conservation Biology 20: 1121–1131.Google Scholar
Hombegowda, HC, Vasudeva, R, Georgi, PM, Shaanker, RU and Ganeshaiah, KN (2002) Breeding types in Nothapodytes nimmoniana Graham. Current Science 83: 1077–1078.Google Scholar
Hsiang, YH, Hertzberg, R, Hecht, S and Liu, LF (1985) Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. Journal of Biological Chemistry 260: 14873–14878.Google Scholar
Hu, Y, Wang, L, Xie, X, Yang, J, Li, Y and Zhang, H (2010) Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis. Biochemical Systematics and Ecology 38: 264–274.CrossRefGoogle Scholar
Jin, Z and Li, J (2007) Genetic differentiation in endangered Heptacodium miconioides Rehd. based on ISSR polymorphism and implications for its conservation. Forest Ecology and Management 245: 130–136.Google Scholar
Kala, CP (2005) Indigenous uses, population density, and conservation of threatened medicinal plants in protected areas of the Indian Himalayas. Conservation Biology 19: 368–378.Google Scholar
Kavitha, P, Kumar, TV, Rajasekharan, PE, Kareem, VKA and Rao, VK (2010) Camptothecin and 9-methoxy camptothecin, anti-cancer alkaloids in Nothapodytes nimmoniana from Western Ghats, India. Journal of Medicinal and Aromatic Plant Science 32: 129–132.Google Scholar
Li, SY and Adair, KT (1994) Camptotheca acuminata Decaisne XI SHU 3ß¾ð (Chinese Happy tree) a promising anti-tumor and anti-viral tree for the 21st century. Nacogdoches, TX: The Tucker Center College of Forestry, Stephen F. Austin State University.Google Scholar
Meyer, W, Mitchell, TG, Freedman, EZ and Vilgays, R (1993) Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. Journal of Clinical Microbiology 31: 2274–2280.CrossRefGoogle ScholarPubMed
Miller, MP (1997) A Windows Program for the Analysis of Allozyme and Molecular Population Genetic Data (TFPGA). Flagstaff, AZ: Department of Biological Sciences, Northern Arizona University.Google Scholar
Nagaoka, T and Ogihara, Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Applied Genetics 94: 597–602.CrossRefGoogle Scholar
Neel, MC and Cummings, MP (2003) Effective of conservation targets in capturing genetic diversity. Conservation Biology 17: 219–229.Google Scholar
Neel, MC and Ellstrand, NC (2003) Conservation of genetic diversity in the endangered plant Eriogonum ovalifolium var. vineum (Polygonaceae). Conservation Genetics 4: 337–352.Google Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70: 3321–3323.Google Scholar
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.CrossRefGoogle ScholarPubMed
Peakall, R and Smouse, PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.CrossRefGoogle Scholar
Potmesil, M (1994) Camptothecin: from bench research to hospital wards. Cancer Research 54: 1431–1439.Google Scholar
Qiuying, Y, Xiu, ZY, Jucai, C, Yuan, CI, Chang, YH and Tsai, CT (2005) Population genetic variation of Nothapodytes foetida (Wight) Sleumer in Taiwan base on amplified fragment-length polymorphism. Journal of the Chinese Society for Horticultural Science 51: 283–293.Google Scholar
Ravikumar, and Ved, DK (2000) Illustrated Field Guide to 100 Red Listed Medicinal Plants of Conservation Concern in Southern India. Bangalore: FRLHT, pp. 261–263.Google Scholar
Reisch, C, Poschold, P and Wingender, R (2003) Genetic variation in Saxifraga paniculata Mill. (Saxifragaceae): molecular evidence for glacial relict endemism in central Europe. Biological Journal of the Linnean Society 80: 11–21.CrossRefGoogle Scholar
Shah, A, Li, DZ, Gao, LM, Li, HT and Moller, M (2008) Genetic diversity within and among populations of the endangered species Taxus fauna (Taxaceae) from Pakistan and implication of its conservation. Biochemical Systematics and Ecology 36: 183–193.CrossRefGoogle Scholar
Sharma, MV, Shaanker, RU, Vasudeva, R and Shivanna, KR (2010) Functional dioecy in Nothapodytes nimmoniana, a distylous species in the Western Ghats. Current Science 99: 1444–1449.Google Scholar
Slatkin, M (1993) Isolation by distance in equilibrium and non-equilibrium populations. Evolution 47: 264–279.Google Scholar
Souza, HAV and Lovato, MB (2010) Genetic diversity and structure of the critically endangered tree Dimorphandra wilsonii and of the widespread in the Brazilian Cerrado Dimorphandra mollis: implications for conservation. Biochemical Systematics and Ecology 38: 49–56.Google Scholar
Su, YJ, Zan, QJ, Wang, T, Ying, ZM and Ye, HG (2008) High ISSR variation in 24 surviving individuals of Apterosperma oblata (Theaceae) endemic to China. Biochemical Systematics and Ecology 36: 619–625.CrossRefGoogle Scholar
Takeuchi, S, Dobashi, K, Fujimoto, S, Tanaka, K, Suzuki, M, Terashima, Y, Hasumi, K, Akiya, K, Negishi, Y and Tamaya, T (1991) A late phase II study of CPT-11 in gynecologic cancers. Research groups of CPT-11 in gynecologic cancers. Japanese Journal of Cancer and Chemotherapy 18: 1681–1689.Google Scholar
Ved, DK (1997) Trade in medicinal plants: the state of our ignorance. Amruth 1: 2–8.Google Scholar
Vladu, B (2000) 7- and 10-Substituted camptothecins: dependence of topoisomerase I-DNA cleavable complex formation and stability on the 7- and 10-substituents. Molecular Pharmacology 57: 243–251.Google ScholarPubMed
Vrijenhoek, RC, Douglas, ME and Meffe, GK (1985) Conservation genetics of endangered fish populations in Arizona. Science 229: 400–402.CrossRefGoogle ScholarPubMed
Winter, P and Kahl, G (1995) Molecular marker technologies for plant improvement. World Journal of Microbiology and Biotechnology 11: 438–448.CrossRefGoogle ScholarPubMed
Wu, YG, Guo, QS, He, JC, Lin, YF, Luo, LJ and Liu, GD (2010) Genetic diversity analysis among and within populations of Pogostemon cablin from China with ISSR and SRAP markers. Biochemical Systematics and Ecology 38: 63–72.Google Scholar
Yeh, FC, Yang, RC and Boyle, T (1999) POPGENE, version 1.31. Microsoft windows based freeware for population genetic analysis. Available at http://www.ualberta.ca/~fyeh/fyeh.Google Scholar
Zietkiewicz, E, Rafalski, A and Labuda, D (1994) Genomic fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176–183.CrossRefGoogle ScholarPubMed
Zong, M, Liu, HL, Qiu, YX, Yang, SZ, Zhao, MS and Fu, CX (2008) Genetic diversity and geographic differentiation in the threatened species Dysosma pleiantha in China as revealed by ISSR analysis. Biochemical Genetics 46: 108–196.CrossRefGoogle Scholar
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