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Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

Published online by Cambridge University Press:  26 April 2021

Yu-Wei Tseng ,
Chi-Chun Huang ,
Chih-Chiang Wang ,
Chiuan-Yu Li  and
Kuo-Hsiang Hung Open the ORCID record for Kuo-Hsiang Hung [Opens in a new window]
Yu-Wei Tseng
Affiliation:
Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan
Chi-Chun Huang
Affiliation:
Taiwan Endemic Species Research Institute, Nantou, Taiwan
Chih-Chiang Wang
Affiliation:
Department of Forestry, Pingtung University of Science and Technology, Pingtung, Taiwan
Chiuan-Yu Li
Affiliation:
Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan Taiwan Endemic Species Research Institute, Nantou, Taiwan
Kuo-Hsiang Hung*
Affiliation:
Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan
*
*Corresponding author. E-mail: [email protected]
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Abstract

Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Keywords

conservation strategyEpilobium nankotaizanensegenetic diversitymicrosatellite marker
Type
Short Communication
Information
Plant Genetic Resources , Volume 19 , Issue 3 , June 2021 , pp. 275 - 278
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

These authors contributed equally to this work.

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