Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-26T12:52:58.244Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic architecture, physio-biochemical characterization and identification of elite cytoplasmic male sterile (pt-CMS) based combiners in developing antioxidant-rich carrot

Published online by Cambridge University Press:  19 January 2022

Hemant Ghemeray Open the ORCID record for Hemant Ghemeray [Opens in a new window] ,
Raj Kumar ,
T. K. Behera ,
V. K. Sharma ,
Saurabh Singh Open the ORCID record for Saurabh Singh [Opens in a new window] ,
Reeta Bhatia Open the ORCID record for Reeta Bhatia [Opens in a new window]  and
S. S. Dey Open the ORCID record for S. S. Dey [Opens in a new window]
Hemant Ghemeray
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Raj Kumar
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
T. K. Behera
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
V. K. Sharma
Affiliation:
Division of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India
Saurabh Singh*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Reeta Bhatia
Affiliation:
Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
S. S. Dey*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
*
Author for correspondence: S. S. Dey, E-mail: [email protected]
Author for correspondence: S. S. Dey, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Existence of genetic divergence, appropriate characterization of breeding lines for economically important traits and determining parents with favourable alleles is the crux of crop genetic improvement programmes. This study is the first report of unravelling genetics and potential of petaloid-type cytoplasmic male sterile (pt-CMS) lines in carrot. Ten pt-CMS lines were crossed with 10 inbreds in line × tester mating fashion to generate 100 testcross progenies. Nutritional profiling of the 100 testcrosses progenies along with 20 parental types was carried out for two consecutive years for eight important traits to identify superior combiners. The pooled analysis revealed that the carotenoid content in root is under the genetic control of major genes (oligogenic). The pooled analysis revealed less than unity value of σ2A/D and σgca2/σsca2 for majority of the traits depicting preponderance of non-additive gene effects. The pt-CMS lines KT-28A, Kt-62A, KT-80A and KT-95A were identified as good combiners for carotenoids. The cross combination, KT-98A × KS-50 identified as the best heterotic combiner for CUPRAC and FRAP content over the years. Similarly, the combinations, KT-62A × KS-21, KT-80A × New Kuroda and KT-62A × KS-59 were found promising across the years for developing nutritionally rich F1 hybrids. The interaction analysis among the different antioxidant traits and plant pigments unveiled the scope of simultaneous improvement.

Keywords

Antioxidant traitscombining abilityDaucus carotagenetic architectureheterosispetaloid CMSyear × genotype interaction
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 6 , December 2021 , pp. 484 - 496
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aditika, , Kanwar, HS, Priyanka, , Singh, S and Singh, S (2020) Heterotic potential, potence ratio, combining ability and genetic control of quality and yield traits in bell pepper under nethouse conditions of NW Himalayas. Agricultural Research 9, 526–535.CrossRefGoogle Scholar
Ahmad, T, Cawood, M, Iqbal, Q, Arino, A, Batool, A, Tariq, RMS, Azam, M and Akhtar, S (2019) Phytochemicals in Daucus carota and their health benefits – review article. Foods 8, 424.CrossRefGoogle ScholarPubMed
Ainsworth, EA and Gillespie, KM (2007) Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols 2, 875–877.CrossRefGoogle ScholarPubMed
Apak, R, Guclu, K, Demirata, B, Ozyurek, M, Celik, SE, Bektas Oglu, B, Berker, KI and Ozyurt, D (2007) Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 12, 1496–1547.CrossRefGoogle ScholarPubMed
Arscott, SA and Tanumihardjo, SA (2010) Carrots of many colors provide basic nutrition and bioavailable phytochemicals acting as a functional food. Comprehensive Reviews in Food Science and Food Safety 9, 223–239.CrossRefGoogle Scholar
Assous, MTM, Abdel-Hady, MM and Medany, GM (2014) Evaluation of red pigment extracted from purple carrots and its utilization as antioxidant and natural food colorants. Annals of Agricultural Sciences 59, 1–7.CrossRefGoogle Scholar
Athanase, N and Rob, M (2019) Gene action and heterosis in F1 clonal progenies of cassava for β-carotene and farmers’ preferred traits. Heliyon 5, e01807.CrossRefGoogle ScholarPubMed
Benzie, IFF and Strain, JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299, 15–23.CrossRefGoogle ScholarPubMed
Bogacz-Radomska, L and Harasym, J (2018) β-Carotene-properties and production methods. Food Quality and Safety 2, 69–74.CrossRefGoogle Scholar
Cortez, R, Luna-Vital, DA, Margulis, D and de Mejia, EG (2016) Natural pigments: stabilization methods of anthocyanins for food applications. Comprehensive Reviews in Food Science and Food Safety 16, 180–198.CrossRefGoogle ScholarPubMed
Halilu, AD, Ado, SG, Aba, DA and Usman, IS (2016) Genetics of carotenoids for provitamin A biofortification in tropical-adapted maize. Crop Journal 4, 313–322.CrossRefGoogle Scholar
Iorizzo, M, Ellison, S, Senalik, D, Zeng, P, Satapoomin, P, Huang, J, Bowman, M, Iovene, M, Sanseverino, W, Cavagnaro, P, Yildiz, M, Macko-Podgórni, A, Moranska, E, Grzebelus, E, Grzebelus, D, Ashrafi, H, Zheng, Z, Cheng, S, Spooner, D, Van Deynze, A and Simon, P (2016) A high-quality carrot genome assembly provides new insights into carotenoids accumulation and asteroid genome evolution. Nature Genetics 48, 657–666. http://doi.org/10.1038/ng.3565CrossRefGoogle Scholar
Jolliffe, IT and Cadima, J (2016) Principal component analysis: a review and recent developments. Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences 374, 20150202.CrossRefGoogle ScholarPubMed
Jourdan, M, Gagné, S, Dubois-Laurent, C, Maghraoui, M, Huet, S, Suel, A, Hamama, L, Briard, M, Peltier, D and Geoffriau, E (2015) Carotenoid content and root color of cultivated carrot: a candidate-gene association study using an original broad unstructured population. PLoS ONE 10, e0116674.CrossRefGoogle ScholarPubMed
Kamiloglu, S, Van Camp, J and Capanoglu, E (2018) Black carrot polyphenols: effect of processing, storage and digestion – an overview. Phytochemistry Reviews 17, 379–395.CrossRefGoogle Scholar
Karabolias, NG, Greenberg, AJ, Barrero, LS, Maron, LG, Shi, Y, Monteverde, E, Pineros, MA and McCouch, SR (2020) Low additive genetic variation in a trait under selection in domesticated rice. G3: Genes Genomes and Genetics 10, 2435–2443.CrossRefGoogle Scholar
Kempthorne, O (1957) An introduction to genetic statistics. New York: John Wiley and Sons, Inc.Google Scholar
Knothe, G and Steidley, KR (2019) Composition of some Apiaceae seed oils includes phytochemicals, and mass spectrometry for fatty acid 2-methoxyethyl esters. European Journal of Lipid Science and Technology 121, 1800386. https://doi.org/10.1002/ejlt.201800386.CrossRefGoogle Scholar
Koley, TK, Singh, S, Khemariya, P, Sarkar, A, Kaur, C, Chaurasia, SNS and Naik, PS (2014) Evaluation of bioactive properties of Indian carrot (Daucus carota L.): a chemometric approach. Food Research International 60, 76–85.CrossRefGoogle Scholar
Leja, M, Kaminska, I, Kramer, M, Maksylewicz-Kaul, A, Kammerer, D, Carle, R and Baranski, R (2013) The content of phenolic compounds and radical scavenging activity varies with carrot origin and root color. Plant Foods for Human Nutrition 68, 163–170.CrossRefGoogle ScholarPubMed
Liu, B, Ou, C, Chen, S, Cao, Q, Zhao, Z, Miao, Z, Kong, X and Zhuang, F (2019) Differentially expressed genes between carrot petaloid cytoplasmic male sterile and maintainer during floral development. Scientific Reports 9, 17384.CrossRefGoogle ScholarPubMed
Morelock, TE, Simon, PW and Peterson, CE (1996) Wisconsin wild: another petaloid male-sterile cytoplasm for carrot. HortScience 31, 887–888.CrossRefGoogle Scholar
Pepra, BB, Parkes, E, Manu-Aduening, J, Kulakow, P, van Biljon, A and Labuschagne, M (2020) Genetic variability, stability and heritability for quality and yield characteristics in provitamin A cassava varieties. Euphytica 216, 31.CrossRefGoogle Scholar
Plunkett, GM, Pimenov, MG, Reduron, JP, Kljuykov, EV, BE, Van WYK, TA, Ostroumova, MJ, Henwood, PM, Tilney, Spalik, K, MF, Watson, BY, Lee, FD, Pu, CJ, Webb, JM, Hart, AD, Mitchell, and Muckensturm, B (2018) Apiaceae. In Kadereit, J and Bittrich, V (eds), Flowering Plants. Eudicots. The Families and Genera of Vascular Plants. Cham: Springer, vol. 15, pp. 9–206.CrossRefGoogle Scholar
Poleshi, CA, Cholin, S, Manikanta, DS and Ambika, DS (2017) Genetic variability for root traits in carrot (Daucus carota L.) evaluated under tropical condition. Annals of Horticulture 12, 224–227.CrossRefGoogle Scholar
Que, F, Hou, X-L, Wang, G-L, Xu, Z-S, Tan, G-F, Li, T, Wang, Y-H, Khadr, A and Xiong, A-S (2019) Advances in research on the carrot, an important root vegetable in the Apiaceae family. Horticulture Research 6, 69.CrossRefGoogle Scholar
Ranganna, S (1979) Manual of Analysis of Fruits and Vegetable Products. New Delhi: Tata McGraw Hill Book Co.Google Scholar
Robinson, HS (1966) Quantitative genetics in relation to breeding on the central of mendalism. Indian Journal of Genetics and Plant Breeding 26, 171–187.Google Scholar
Robison, MM and Wolyn, DJ (2006) Petaloid-type cms in carrot is not associated with expression of atp8 (orfB). Theoretical and Applied Genetics 112, 1496.CrossRefGoogle Scholar
Rong, J, Lammers, Y, Strasburg, JL, Schidlo, NS, Ariyurek, Y, de Jong, TJ, Klinkhamer, PGL, Smulders, MJM and Vrieling, K (2014) New insights into domestication of carrot from root transcriptome analyses. BMC Genomics 15, 895.CrossRefGoogle ScholarPubMed
R Studio Team (2020) RStudio: Integrated Development for R. Boston, MA: RStudio, PBC. Available at http://www.rstudio.com/.Google Scholar
SAS Institute Inc (2013) SAS Online Doc, Version 9.4. Cary, NC. https://www.sas.com/en_in/home.htmlGoogle Scholar
Sehgal, N and Singh, S (2018) Progress on deciphering the molecular aspects of cell-to-cell communication in Brassica self-incompatibility response. 3Biotech 8, 347.Google ScholarPubMed
Selvakumar, R and Kalia, P (2018) Nutra-rich Carrot production in tropical and temperate regions of India. Findings in Agricultural Research and Management (FARM) Journal 2, 7–13.Google Scholar
Sharma, KD, Karki, S, Thakur, NS and Attri, S (2012) Chemical composition, functional properties and processing of carrot – a review. Journal of Food Science and Technology 49, 22–32.CrossRefGoogle ScholarPubMed
Singh, S and Vidyasagar, (2012) Effect of common salt (NaCl) sprays to overcome the self-incompatibility in the S-allele lines of Brassica oleracea var. capitata L. SABRAO Journal of Breeding and Genetics 44, 339–348.Google Scholar
Singh, S, Bhatia, R, Kumar, R, Sharma, K, Dash, S and Dey, SS (2018 a) Cytoplasmic male sterile and doubled haploid lines with desirable combining ability enhances the concentration of important antioxidant attributes in Brassica oleracea. Euphytica 214, 207.CrossRefGoogle Scholar
Singh, S, Singh, R, Thakur, P and Kumar, R (2018 b) Phytochemicals, functionality and breeding for enrichment of cole vegetables (Brassica oleraceaL.). In Petropoulos, SA, Ferreira, ICFR and Barros, L (eds), Phytochemicals in Vegetables: A Valuable Source of Bioactive Compounds. UAE: Bentham Science Publishers, pp. 256–295.Google Scholar
Singh, S, Dey, SS, Bhatia, R, Kumar, R, Sharma, K and Behera, TK (2019 a) Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites. PLoS ONE 14, e0210772.CrossRefGoogle ScholarPubMed
Singh, S, Dey, SS, Bhatia, R, Kumar, R and Behera, TK (2019 b) Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding. Plant Reproduction 32, 231–256.CrossRefGoogle ScholarPubMed
Singh, S, Dey, SS, Kumar, R, Bhatia, R, Ghemeray, H and Behera, TK (2019 c) Genetic analysis and interaction among CUPRAC, FRAP, phytochemical and phenotypic traits in cauliflower (Brassica oleracea var. botrytis L.). International Journal of Chemical Studies 7, 1484–1494.Google Scholar
Singh, S, Bhatia, R, Kumar, R, Das, A, Ghemeray, H, Behera, TK and Dey, SS (2021) Characterization and genetic analysis of OguCMS and doubled haploid based large genetic arsenal of Indian cauliflowers (Brassica oleracea var. botrytis L.) for morphological, reproductive and seed yield traits revealed their breeding potential. Genetic Resources and Crop Evolution 68, 1603–1623.CrossRefGoogle Scholar
Ssemakula, G, Dixon, AGO and Mazia-Dixon, B (2007) Stability of total carotenoid concentration and fresh yield of selected yellow-fleshed cassava (Manihot esculenta Crantz). Journal of Tropical Agriculture 45, 14–20.Google Scholar
Szklarczyk, M, Szymański, M, Wójcik-Jagła, M, Simon, PW, Weihe, A and Borner, T (2014) Mitochondrial atp9 genes from petaloid male-sterile and male-fertile carrots differ in their status of heteroplasmy, recombination involvement, post-transcriptional processing as well as accumulation of RNA and protein product. Theoretical and Applied genetics 127, 1689–1701.CrossRefGoogle ScholarPubMed
Yoo, KS, Bang, H, Pike, L, Patil, BS and Lee, EJ (2020) Comparing carotene, anthocyanins, and terpenoid concentrations in selected carrot lines of different colors. Horticulture Environment and Biotechnology 61, 385–393.CrossRefGoogle Scholar
Supplementary material: File

Ghemeray et al. supplementary material

Ghemeray et al. supplementary material

Download Ghemeray et al. supplementary material(File)
File 884.8 KB