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Genetic analysis of Cheirostylis species based on microsatellite markers

Published online by Cambridge University Press:  02 October 2014

Supajit Sraphet ,
Anuwat Saengsri ,
Duncan R. Smith  and
Kanokporn Triwitayakorn
Supajit Sraphet*
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
Anuwat Saengsri
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
Duncan R. Smith
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
Kanokporn Triwitayakorn*
Affiliation:
Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand
*
* Corresponding authors: E-mail: [email protected]; [email protected]
* Corresponding authors: E-mail: [email protected]; [email protected]
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Abstract

Microsatellite markers specific to Cheirostylis yunnanensis Rolfe were developed using an enriched genomic DNA library technique. The library was constructed using (AG)20 and (CAG)20 oligonucleotide repeats. A total of 48 primer pairs were designed and tested with 48 C. yunnanensis Rolfe samples, resulting in 11 polymorphic loci. The number of alleles per locus ranged from 2 to 12, with an average of six alleles. The observed and expected heterozygosity ranged from 0.0426 to 0.8085 and 0.0421 to 0.9078, respectively. Of the 11 polymorphic loci, three showed a significant deviation from Hardy–Weinberg equilibrium and one exhibited linkage disequilibrium. Cross-species amplification was tested with five samples of Cheirostylis of unknown species resulting in eight loci that could be amplified, with the number of alleles ranging from one to two. The microsatellite markers developed in this study will be useful for the genetic analysis of C. yunnanensis in order to differentiate species as well as to establish a conservation plan for this species.

Keywords

Cheirostylis spcross-amplificationenriched genomic librarymicrosatellite
Type
Short Communication
Information
Plant Genetic Resources , Volume 13 , Issue 3 , November 2015 , pp. 286 - 289
Copyright
Copyright © NIAB 2014 

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