Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-02T22:05:36.306Z Has data issue: false hasContentIssue false

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

Published online by Cambridge University Press:  25 May 2011

Zuzana Šramková
Affiliation:
Institute of Biochemistry, Nutrition and Health Protection, Slovak University of Technology, Bratislava, Slovakia
Edita Gregová*
Affiliation:
Plant Production Research Center, Plant Production Research Institute, Pieštany, Slovakia
Svetlana Šliková
Affiliation:
Plant Production Research Center, Plant Production Research Institute, Pieštany, Slovakia
Ernest Šturdík
Affiliation:
Department of Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovakia
*
*Corresponding author. E-mail: [email protected]

Abstract

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Type
Research Article
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bushuk, W (1998) Wheat breeding for end-product use. Euphytica 100: 137145.CrossRefGoogle Scholar
Dotlačil, L, Bradová, J, Hermuth, J and Stehno, Z (2003) Diversity of HMW-Glu alleles in landraces and cultivars of winter wheat (Triticum aestivum), spelt (Triticum spelta) and emmer (Triticum dicoccoides). In: Pogna, NE, Romano, M, Pogna, EA and Galterio, G (eds) Proceedings of the 10th International Wheat Genetics Symposium. 1–6 September 2003, Paestum, Italy, pp. 475477.Google Scholar
Draper, SR (1987) ISTA variety committee report of the working group for biochemical tests for cultivar identification 1983–1986. Seed Science and Technology 15: 431434.Google Scholar
Gálová, Z, Michalík, I, Knoblochová, H and Gregová, E (2002) Variation in HMW glutenin subunits of different species of wheat. Rostlinna Výroba 48: 1519.Google Scholar
Gobaa, S, Brabant, C, Kleijer, G and Stamp, P (2008) Effect of the 1BL.1RS translocation and of the Glu-B3 variation on fifteen quality tests in a doubled haploid population of wheat (Triticum aestivum L.). Journal of Cereal Science 48: 598603.Google Scholar
Graybosch, RA (2001) Uneasy unions: quality effects of rye chromatin transfers to wheat. Journal of Cereal Science 33: 316.CrossRefGoogle Scholar
Li, Y, Huang, Ch, Sui, X, Fan, Q, Li, G and Chu, X (2009) Genetic variation of wheat glutenin subunits between landraces and varieties and their contributions to wheat quality improvement in China. Euphytica 169: 159168.Google Scholar
Liang, D, Tang, J, Pena, RJ, Singh, R, He, X, Shen, X, Yao, D, Xia, X and He, Z (2010) Characterization of CIMMYT bread wheats for high- and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers. Euphytica 172: 235250.Google Scholar
Nakamura, H (2001) Genetic diversity of high-molecular-weight glutenin subunit compositions in landraces of hexaploid wheat from Japan. Euphytica 120: 227234.CrossRefGoogle Scholar
Payne, PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annual Review of Plant Physiology 38: 141153.Google Scholar
Payne, PI and Lawrence, GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1, which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Research Communications 11: 2935.Google Scholar
Payne, PI, Nightigale, MA, Krattinger, AF and Holt, LM (1987) The relationship between the HMW glutenin subunit composition and the breadmaking quality of British-grown wheat varieties. Journal of the Science of Food and Agriculture 40: 5165.CrossRefGoogle Scholar
Redaelli, R, Ng, KW and Pogna, NE (1997) Allelic variation at the storage protein loci of 55 US-grown white wheats. Plant Breeding 116: 429436.Google Scholar
Ren, Y, Wang, T, Xu, Z-B, Yang, Z-J and Ren, Z-L (2008) Molecular characterization of a novel HMW-GS 1Dx5′ associated with good bread making quality (Triticum aestivum L.) and the study of its unique inheritance. Genetic Resources and Crop Evolution 55: 585592.Google Scholar
Shan, X, Clayshulte, SR, Haley, SD and Byrne, PF (2007) Variation for glutenin and waxy alleles in the US hard winter wheat germplasm. Journal of Cereal Science 45: 199208.Google Scholar
Wrigley, CW (1992) Identification of cereal varieties by gel electrophoresis of the grain proteins. In: Linskens, HF and Jackson, JF (eds) Seed Analysis. Heidelberg: Springer-Verlag, pp. 1741.CrossRefGoogle Scholar
Yuan, Z-W, Chen, Q-J, Zhang, L-Q, Yan, Z-H, Zheng, Y-L and Liu, D-C (2009) Molecular characterization of two silenced y-type genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum. Journal of Integrative Plant Biology 51: 9399.CrossRefGoogle ScholarPubMed
Zheng, S, Byrne, PF, Bai, G, Shan, X, Reid, SD, Haley, SD and Seabourn, BW (2009) Association analysis reveals effects of wheat glutenin alleles and rye translocations on dough-mixing properties. Journal of Cereal Science 50: 283290.Google Scholar