Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-14T21:25:37.473Z Has data issue: false hasContentIssue false
  • English
  • Français

Development and characterization of Triticum turgidum–Aegilops umbellulata amphidiploids

Published online by Cambridge University Press:  18 September 2018

Zhongping Song ,
Shoufen Dai ,
Yanni Jia ,
Li Zhao ,
Liangzhu Kang ,
Dengcai Liu ,
Yuming Wei ,
Youliang Zheng  and
Zehong Yan
Zhongping Song
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Shoufen Dai
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Yanni Jia
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Li Zhao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Liangzhu Kang
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Dengcai Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Yuming Wei
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Youliang Zheng
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
Zehong Yan*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The U genome of Aegilops umbellulata is an important basic genome of genus Aegilops. Direct gene transfer from Ae. umbellulata into wheat is feasible but not easy. Triticum turgidum–Ae. umbellulata amphidiploids can act as bridges to circumvent obstacles involving direct gene transfer. Seven T. turgidum–Ae. umbellulata amphidiploids were produced via unreduced gametes for spontaneous doubling of chromosomes of triploid T. turgidum–Ae. umbellulata F1 hybrid plants. Seven pairs of U chromosomes of Ae. umbellulata were distinguished by fluorescence in situ hybridization (FISH) probes pSc119.2/(AAC)5 and pTa71. Polymorphic FISH signals were detected in three (1U, 6U and 7U) of seven U chromosomes of four Ae. umbellulata accessions. The chromosomes of the tetraploid wheat parents could be differentiated by probes pSc119.2 and pTa535, and identical FISH signals were observed among the three accessions. All the parental chromosomes of the amphidiploids could be precisely identified by probe combinations pSc119.2/pTa535 and pTa71/(AAC)5. The T. turgidum–Ae. umbellulata amphidiploids possess valuable traits for wheat improvement, such as strong tillering ability, stripe rust resistance and seed size-related traits. These materials can be used as media in gene transfers from Ae. umbellulata into wheat.

Keywords

amphidiploidsFISH (fluorescent in situ hybridization)T. turgidum ssp.U genomeunreduced gametes
Type
Research Article
Information
Plant Genetic Resources , Volume 17 , Issue 1 , February 2019 , pp. 24 - 32
Copyright
Copyright © NIAB 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

Contributed equally to this work.

References

Badaeva, ED, Friebe, B and Bikram, SG (1996) Genome differentiation in Aegilops. 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid species. Genome 39: 293–306.Google Scholar
Bansal, M, Kaur, S, Dhaliwal, HS, Bains, NS, Bariana, HS, Chhuneja, P and Bansal, UK (2017) Mapping of Aegilops umbellulata-derived leaf rust and stripe rust resistance loci in wheat. Plant Pathology 66: 38–44.Google Scholar
Blanco, A, Simeone, R and Resta, P (1987) The addition of Dasypyrum villosum (L.) Candargy chromosomes to durum wheat (Triticum durum Desf.). Theoretical and Applied Genetics 74: 328–333.Google Scholar
Cakmak, I, Tolay, I, Özkan, H, Özdemir, A and Braun, HJ (1999) Variation in zinc efficiency among and within Aegilops species. Journal of Plant Nutrition and Soil Science 162: 257–262.Google Scholar
Chhuneja, P, Kaur, S, Goel, RK, Aghaee-Sarbarzeh, M, Prashar, M and Dhaliwal, HS (2008) Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk. to bread wheat (Triticum aestivum l.). Genetic Resources and Crop Evolution 55: 849–859.Google Scholar
Cuadrado, Á and Jouve, N (2010) Chromosomal detection of simple sequence repeats (SSRs) using nondenaturing FISH (ND-FISH). Chromosoma 119: 495–503.Google Scholar
Dai, SF, Zhao, L, Xue, XF, Jia, YN, Liu, DC, Pu, ZJ, Zheng, YL and Yan, ZH (2015) Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata. Plant Genetic Resources: Characterization and Utilization 13: 186–189.Google Scholar
Edae, EA, Olivera, PD, Jin, Y and Rouse, MN (2017) Genotyping-by-sequencing facilitates a high-density consensus linkage map for Aegilops umbellulata, a wild relative of cultivated wheat. G3, Genes, Genomes, Genetics 7: 1551–1561.Google Scholar
Hadzhiivanova, B, Bozhanova, B and Dechev, D (2012) Interspecific hybridization between durum wheat and Aegilops umbellulata (Zhuk.). Bulgarian Journal of Agricultural Science 18: 713–721.Google Scholar
Hao, M, Luo, JT, Yang, M, Zhang, LQ, Yan, ZH, Yuan, ZW, Zheng, YL, Zhang, HG and Liu, DC (2011) Comparison of homoeologous chromosome pairing between hybrids of wheat genotypes Chinese Spring ph1b and Kaixian-luohanmai with rye. Genome 54: 959–964.Google Scholar
Hao, M, Luo, JT, Zhang, LQ, Yuan, ZW, Yang, YW, Wu, M, Chen, WJ, Zheng, YL, Zhang, HG and Liu, DC (2013) Production of hexaploid triticale by a synthetic hexaploid wheat-rye hybrid method. Euphytica 193: 347–357.Google Scholar
Hao, M, Luo, J, Zeng, D, Zhang, L, Ning, S, Yuan, Z, Yan, Z, Zhang, H, Zheng, Y, Feuillet, C, Choulet, F, Yen, Y, Zhang, L and Liu, D (2014) QTug.sau-3B is a major quantitative trait locus for wheat hexaploidization. G3, Genes, Genomes, Genetics 4: 1943–1953.Google Scholar
Jauhar, PP (2003) Formation of 2n gametes in durum wheat haploids: sexual polyploidization. Euphytica 133: 81–94.Google Scholar
Jauhar, PP (2007) Meiotic restitution in wheat polyhaploids (amphiploids): a potent evolutionary force. Journal of Heredity 98: 188–193.Google Scholar
Kato, A (1999) Air drying method using nitrous oxide for chromosome counting in maize. Biotechnic & Histochemistry 74: 160–166.Google Scholar
Komuro, S, Endo, R, Shikata, K and Kato, A (2013) Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome 56: 131–137.Google Scholar
Kwiatek, M, Wiśniewska, H and Apolinarska, B (2013) Cytogenetic analysis of Aegilops chromosomes, potentially usable in triticale (×Triticosecale witt.) breeding. Journal of Applied Genetics 54: 147–155.Google Scholar
Mirzaghaderi, G, Houben, A and Badaeva, ED (2014) Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat. Molecular Cytogenetics 7: 91.Google Scholar
Sears, ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symposium in Biology 9: 1–21.Google Scholar
Storme, DS and Geelen, D (2013) Sexual polyploidization in plants-cytological mechanisms and molecular regulation. New Phytologist 198: 670–684.Google Scholar
Tang, ZX, Yang, ZJ and Fu, SL (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics 55: 313–318.Google Scholar
Tiwari, VK, Rawat, N, Neelam, K, Randhawa, GS, Singh, K, Chhuneja, P and Dhaliwal, HS (2008) Development of Triticum trugidum ssp. durum-Aegilops longissima amphiploids with high iron and zinc content through unreduced gamete formation in F 1 hybrids. Genome 51: 757–766.Google Scholar
Vardi, A and Zohary, D (1967) Introgression in wheat via triploid hybrids. Heredity 22: 541–560.Google Scholar
Wang, SW, Yin, LN, Tanaka, H, Tanaka, K and Tsujimoto, H (2011) Wheat-Aegilops chromosome addition lines showing high iron and zinc contents in grains. Breeding Science 61: 189–195.Google Scholar
Wellings, C and Bariana, H (2004) Assessment scale for recording stripe rust responses in field trials. Cereal Rust Report Season 2: 1–2.Google Scholar
Zaharieva, M, Cortéz, A, Rosas, V, Cano, S, Delgado, R and Mujeeb-kazi, A (2003) Triticum durum × Aegilops umbellulata hybridization. Annual Wheat Newsletter 49: 71–73.Google Scholar
Zhang, H, Jia, J, Gale, MD and Devos, KM (1998) Relationships between the chromosomes of Aegilops umbellulata and wheat. Theoretical and Applied Genetics 96: 69–75.Google Scholar
Zhang, LQ, Yen, Y, Zheng, YL and Liu, DC (2007) Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines. Sexual Plant Reproduction 20: 159–166.Google Scholar
Zhang, LQ, Yan, ZH, Dai, SF, Chen, QJ, Yuan, ZW, Zheng, YL and Liu, DC (2008) The crossability of Triticum turgidum with Aegilops tauschii. Cereal Research Communications 37: 417–427.Google Scholar
Zhang, LQ, Liu, DC, Zheng, YL, Yan, ZH, Dai, SF, Li, YF, Jiang, Q, Ye, YQ and Yen, Y (2010) Frequent occurrence of unreduced gametes in Triticum turgidum–Aegilops tauschii hybrids. Euphytica 172: 285–294.Google Scholar
Zhu, ZD, Zhou, RH, Kong, XY, Dong, YC and Jia, JZ (2006) Microsatellite marker identification of a Triticum aestivum-Aegilops umbellulata substitution line with powdery mildew resistance. Euphytica 150: 149–153.Google Scholar
Supplementary material: File

Song et al. supplementary material

Song et al. supplementary material 1

Download Song et al. supplementary material(File)
File 2.7 MB
Supplementary material: File

Song et al. supplementary material

Song et al. supplementary material 2

Download Song et al. supplementary material(File)
File 2.2 MB