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Changes in the genetic diversity of barley of Nordic and Baltic origin, studied by isozyme electrophoresis

Published online by Cambridge University Press:  27 June 2007

Agnese Kolodinska Brantestam ,
Roland von Bothmer ,
Isaak Rashal  and
Jens Weibull
Agnese Kolodinska Brantestam*
Affiliation:
Swedish University of Agricultural Sciences, Department of Crop Science, PO Box 44, SE-230 53 Alnarp, Sweden
Roland von Bothmer
Affiliation:
Swedish University of Agricultural Sciences, Department of Crop Science, PO Box 44, SE-230 53 Alnarp, Sweden
Isaak Rashal
Affiliation:
Laboratory of Plant Genetics, Institute of Biology, University of Latvia, LV-2169 Salaspils, Latvia
Jens Weibull
Affiliation:
Swedish University of Agricultural Sciences, Department of Crop Science, PO Box 44, SE-230 53 Alnarp, Sweden
*
*Corresponding author. E-mail: [email protected]
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Abstract

In this study an evaluation was made of changes in the genetic variation of barley (Hordeum vulgare L.) of Nordic and Baltic origin, from the end of the 19th century until today. A comparison of Nordic and Baltic material with exotic material that has not been subjected to intense selection was also made. A total of 293 accessions, including 160 Nordic and 80 Baltic landraces, cultivars and breeding lines, and another 53 landraces of exotic origin (Central Asia), were surveyed using isozyme starch gel electrophoresis. For the four isozymes studied, 28 alleles at 11 loci were observed. The average total genetic diversity value (HT) for individual loci ranged between 0 and 0.519. In the exotic material nine loci were polymorphic and two monomorphic, compared to seven polymorphic and four monomorphic loci in the Nordic and Baltic material. Some of the rare alleles were detected only in the exotic material. The studied isozyme loci of Nordic and Baltic material indicated that modern cultivars have a lower average genetic diversity value compared to the landraces, old and exotic material.

Keywords

cultivated barleygenetic erosionHordeum vulgare Lisozyme variation
Type
Research Article
Information
Plant Genetic Resources , Volume 1 , Issue 2-3 , August 2003 , pp. 143 - 149
Copyright
Copyright © NIAB 2003

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References

Baum, BR, Thompson, BK, Grant Bailey, L and Brown, M (1981) Barley Register. Canada: Research Branch Agriculture.Google Scholar
Bernardo, AA, Luque, A, Cuadrado, A, Negro, A, Jouve, N and Soler, C (1997) The assessment of genetic variation in Spanish primitive cultivars of barley, Hordeum vulgare L. by a combination of isozymes and hordeins. Genetic Resources and Crop Evolution 44: 217–226.CrossRefGoogle Scholar
Brown, AHD (1978) Isozymes, plant population genetic structure and genetic conservation. Theoretical and Applied Genetics 52: 145–157.Google Scholar
Clegg, MT (1989) Molecular diversity in plant populations. In: Brown, AHD, Clegg, MT, Kahler, AL and Weir, BS (eds) Plant Populations, Genetics, Breeding, and Genetic Resources. Sunderland, MA: Sinauer, pp. 98–115.Google Scholar
Davila, JA, de la Hoz, S, Loarce, Y and Ferrer, E (1998) The use of random amplified microsatellite polymorphic DNA and coefficients of parentage to determine genetic relationships in barley. Genome 41: 477–486.CrossRefGoogle Scholar
Dawson, IK, Chalmers, KJ, Waugh, R and Powel, W (1993) Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD. Molecular Ecology 2: 151–159.Google Scholar
Doebley, J (1989) Isoenzyme evidence and the evolution of crop plants. In: Soltis, DE and Soltis, PS (eds) Isozymes in Plant Biology. London: Chapman and Hall, pp. 165–191.CrossRefGoogle Scholar
Fischbeck, G (1992) Barley cultivar development in Europe–success in past and possible changes in the future. Barley Genetics 4: 885–901.Google Scholar
Gaike, M (1992) Spring barley. In: Holms, I (ed.) Crop Breeding in Latvia. Riga: Avots, pp. 53–63 [in Latvian].Google Scholar
Graner, A, Werner, FL and Melchinger, AE (1994) Relationships among European barley germplasm: II. Comparison of RFLP and pedigree data. Crop Science 34: 1199–1205.Google Scholar
Hvid, S and Nielsen, G (1977) Esterase isoenzyme variants in barley. Hereditas 87: 155–162.Google Scholar
Jasieniuk, M and Maxwell, BD (2001) Plant diversity: new insights from molecular biology and genomics technologies. Weed Science 49: 257–265.Google Scholar
Kahler, AL, Heath-Pagliuso, S and Allard, RW (1981) Genetics of isozyme variants in barley. II. 6-Phosphogluconate dehydrogenase, glutamate oxalate transaminase, and acid phosphatase. Crop Science 21: 536–540.Google Scholar
Koebner, RMD, Donini, P, Reeves, JC, Cooke, RJ and Law, JR (2003) Temporal flux in the morphological and molecular diversity of UK barley. Theoretical and Applied Genetics 106: 550–558.CrossRefGoogle ScholarPubMed
Konishi, T (1989) Geographical differences of isozymes in barley. In: Iyama, S and Takeda, G (eds) Proceedings of the 6th International Congress of SABRAO. Tokyo: Organ. Comm. SABRAO, pp. 163–166.Google Scholar
Konishi, T and Yoshimi, R (1993) Geographical distribution of isozyme alleles for phosphogluconate dehydrogenase in barley. Genome 36, 512–516.CrossRefGoogle ScholarPubMed
Konishi, T, Suh, J, Park, KG and Matsuura, S (1993) Comparative studies in isozyme genotypes between Korean and Japanese barley varieties. Crop Production and Improvement Technology in Asia. Korea: Korean Society of Crop Science, pp. 405–411.Google Scholar
Linde-Laursen, IB, Nielsen, G and Johansen, HB (1987) Distribution of isoenzyme markers at 37 loci in a pedigree of European spring barley. Hereditas 106, 241–251.Google Scholar
Liu, F, von Bothmer, R and Salomon, B (1999) Genetic diversity among East Asian accessions of the barley core collection as revealed by six isozyme loci. Theoretical and Applied Genetics 98, 1226–1233.Google Scholar
Liu, F, von Bothmer, R and Salomon, B (2000) Genetic diversity in European accessions of the Barley Core Collection as detected by isozyme electrophoresis. Genetic Resources and Crop Evolution 47, 571–581.Google Scholar
Liu, F, Sun, GL, Salomon, B and von Bothmer, R (2001) Distribution of allozymic alleles and genetic diversity in the American Barley Core Collection. Theoretical and Applied Genetics 102, 606–615.Google Scholar
Manninen, O and Nissilä, E (1997) Genetic diversity among Finnish six-rowed barley cultivars based on pedigree information and DNA markers. Hereditas 126, 87–93.Google Scholar
Matsuura, S and Konishi, T (1986) Genetic variation in esterase isozymes of Japanese barley cultivars. Barley Genetics 5, 155–160.Google Scholar
Melchinger, AE, Graner, A, Singh, M and Messmer, MM (1994) Relationships among European Barley germplasm: I Genetic diversity among winter and spring cultivars revealed by RFLPs. Crop Science 34, 1191–1199.Google Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Science of the USA 70, 3321–3323.CrossRefGoogle ScholarPubMed
Nei, M (1987) Molecular Evolutionary Genetics. New York: Columbia University Press, pp. 176–191.Google Scholar
Nielsen, G and Johansen, H (1986) Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isoenzyme loci of 47 reference varieties. Euphytica 35, 717–728.Google Scholar
Parzies, HK, Spoor, W and Ennos, RA (2000) Genetic diversity of barley landrace accessions (Hordeum vulgare ssp. vulgare) conserved for different lengths of time in ex situ gene banks. Heredity 84, 476–486.Google Scholar
Persson, G (1997) Förädling av vårkorn i Sverige från för till nu. In: Olsson, G (ed.) Den svenska vä xtförädlingens historia. Stockholm: Klungl. Skogs-och Lantbruksakademien, pp. 187–204 [in Swedish].Google Scholar
Plucknett, DL, Smith, NJH, Williams, JT and Anishetty, NM (1987) Gene Banks and the World's Food. Princeton, NJ: Princeton University Press.Google Scholar
Sjakste, TG, Rashal, I and Röder, MS (2003) Inheritance of microsatellite alleles in pedigrees of Latvian barley varieties and related European ancestors. Theoretical and Applied Genetics 106, 539–549.Google Scholar
Soltis, DE and Soltis, PS (eds) (1989) Isozymes in Plant Biology. London: Chapman and Hall, pp. 87–189.Google Scholar
Thomas, WTB, Powel, l, Waugh, R, Chalmers, KJ, Barua, UM, Jack, P, Lea, V, Forster, BP, Swanston, JS, Ellis, RP, Hanson, PR and Lance, RCM (1995) Detection of qualitative trait loci for agronomic, yield, grain and disease characters in spring barley (Hordeum vulgare L.). Theoretical and Applied Genetics 91, 1037–1047.Google Scholar
Turuspekov, Y, Nakamura, K, Yoshikawa, R and Tuberosa, R (2001) Genetic diversity of Japanese barley cultivars based on SSR analysis. Breeding Science 51, 215–218.Google Scholar
Yeh, FH, Yang, RC, Boyle, TBJ, Ye, ZH and Mao, JX (1999) POPGENE, the User-friendly Shareware for Population Genetic Analysis. Canada: Molecular Biology and Biotechnology Centre, University of Alberta, Canada.Google Scholar