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Assessment of the genetic diversity and population structure of apricot (Prunus armeniaca L.) germplasm of the Northwestern Himalaya using SSR markers

Published online by Cambridge University Press:  17 September 2021

Aijaz A. Wani Open the ORCID record for Aijaz A. Wani [Opens in a new window] ,
Khalid Hussain ,
Showkat A. Zargar Open the ORCID record for Showkat A. Zargar [Opens in a new window] ,
Faizan Ahmad ,
Reetika Mahajan ,
Sajad Majeed Zargar Open the ORCID record for Sajad Majeed Zargar [Opens in a new window]  and
Anzar A. Khuroo
Aijaz A. Wani*
Affiliation:
Cytogenetics and Reproductive Biology Laboratory, Department of Botany, University of Kashmir, Srinagar 190006, J & K, India
Khalid Hussain
Affiliation:
Department of Botany, Centre for Biodiversity & Taxonomy, University of Kashmir, Srinagar190006, J & K, India
Showkat A. Zargar
Affiliation:
Cytogenetics and Reproductive Biology Laboratory, Department of Botany, University of Kashmir, Srinagar 190006, J & K, India Department of Botany, Punjabi University, Patiala, Punjab 147002, India
Faizan Ahmad
Affiliation:
Krishi Vigyan Kendra, SKUAST-K, Kargil, Ladakh 194103, J & K, India
Reetika Mahajan
Affiliation:
Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology, Shalimar, Srinagar, J & K, India
Sajad Majeed Zargar
Affiliation:
Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology, Shalimar, Srinagar, J & K, India
Anzar A. Khuroo
Affiliation:
Department of Botany, Centre for Biodiversity & Taxonomy, University of Kashmir, Srinagar190006, J & K, India
*
Author for correspondence: Aijaz A. Wani, E-mail: [email protected], [email protected]
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Abstract

Apricot is considered an ecologically and economically important tree species of the stone-fruit crops that is widely grown in temperate regions of the world. Very few studies on apricot genetic diversity assessment have been carried out from the regions of Kashmir and Ladakh. In this backdrop, the present study was carried out to analyse the genetic diversity and population structure of 120 apricot genotypes collected from both the regions using 21 SSR markers. A total of 52 alleles were amplified with average values of marker index (MI) = 0.7084, resolving power (RP) = 2.8690, polymorphism information content (PIC) = 0.3132, Na = 2.317, Ne = 1.720, I = 0.572, Ho = 0.284, He = 0.360 and an average polymorphism of 91.2% per assay indicating high level of genetic diversity. The neighbour-joining (NJ) dendrogram generated three main clusters among selected apricot genotypes independent of their geographical locations. Interestingly, the result of the dendrogram coincides with the results of structure analysis which showed that the 120 apricot genotypes could be assigned to three (K = 3) sub-populations and the grouping of genotypes did not follow their geographical location suggesting that they share the same genetic pool. Moreover, analysis of molecular variance showed that 73% of the variation was attributed to differences within the individuals, 25% among individuals while only 2% of the variation was observed among the populations. The present study represents the most comprehensive analysis of the genetic diversity and population structure of apricot genotypes in Kashmir and Ladakh regions of India.

Keywords

AMOVAapricotgenetic diversityheterozygositySSRstructure analysis
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 5 , October 2021 , pp. 384 - 393
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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