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Aphid feeding response and microsatellite-based genetic diversity amongdiploid Brachypodium distachyon (L.) Beauv accessions

Published online by Cambridge University Press:  01 April 2009

Perumal Azhaguvel ,
Wanlong Li ,
Jackie C. Rudd ,
Bikram S. Gill ,
G. J. Michels Jr.  and
Yiqun Weng
Perumal Azhaguvel
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Wanlong Li
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
Jackie C. Rudd
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Bikram S. Gill
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
G. J. Michels Jr.
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Yiqun Weng*
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

False brome grass, Brachypodium distachyon (L.) Beauv, has been proposedas a new model species to bridge rice and temperate cereal crops for genomics research.However, much basic information for this species is still lacking. In this study, sixdiploid B. distachyon(2n = 2x = 10)accessions (Bd1-1, Bd2-3, Bd3-1, Bd18-1, Bd21 and BD29) were evaluated for their responseto infestation by two cereal aphid pests of common wheat (Triticumaestivum L.): the greenbug, Schizaphis graminum Rondani, and theRussian wheat aphid (RWA), Diuraphis noxia Mordvilko. Through databasemining of B. distachyon expressed sequence tag (EST) and genomic DNAsequences, 160 EST- and 21 genomic microsatellite markers were developed and used toevaluate genetic diversity among the B. distachyon accessions. All sixaccessions were resistant to RWA biotype RWA1 but showed distinct responses to feeding bygreenbug biotypes C and E, as well as RWA2 RWAs. Although microsatellite-based geneticdiversity among different accessions was generally low, Bd1-1 and BD29 were the mostdiverged from the other four lines. The genetic divergence was correlated withgeographical distances between the Brachypodium accessions. Comparison ofsimple sequence repeat polymorphisms in three inbred lines (Bd2-3, Bd3-1 and Bd18-1) withtheir respective original parental lines revealed no effect of inbreeding on geneticdiversity. Phylogenetic analysis suggested that Aegilops tauschii (Coss.)Schmal., the D genome donor of common wheat, was closer to B. distachyonthan to rice. The greenbug - B. distachyon system seemsto be a model of choice for plant–aphid interaction studies in the grassgenome.

Keywords

aphid resistanceBrachypodium distachyonDiuraphis noxiagenetic diversitymicrosatelliteSchizaphis graminum
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 1 , April 2009 , pp. 72 - 79
Copyright
Copyright © NIAB 2008

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References

Allwood, JW, Ellis, DI, Heald, JK, Goodacre, R and Mur, LA (2006) Metabolomic approaches reveal that phosphatidic and phosphatidyl glycerol phospholipids are major discriminatory non-polar metabolites in responses by Brachypodium distachyon to challenge by Magnaporthe grisea. Plant Journal 46: 351–368.CrossRefGoogle ScholarPubMed
Altschul, SF, Gish, W, Miller, W, Myers, EW and Lipman, DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.Google Scholar
Bennett, MD and Leitch, IJ (2005) Nuclear DNA amounts in angiosperms: progress, problems and prospects. Annals of Botany 95: 45–90.CrossRefGoogle Scholar
Bossolini, E, Wicker, T, Knobel, PA and Keller, B (2007) Comparison of orthologous loci from small grass genomes Brachypodium and rice: implications for wheat genomics and grass genome annotation. Plant Journal 49: 704–717.CrossRefGoogle ScholarPubMed
Botstein, D, White, RL, Skolnick, M and Davis, RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314–331.Google ScholarPubMed
Burd, JD and Porter, DR (2006) Biotypic diversity in greenbug (Hemiptera: Aphididae): characterizing new virulence and host associations. Journal of Economic Entomology 99: 959–965.Google Scholar
Burd, JD, Burton, RL and Webster, JA (1993) Evaluation of Russian wheat aphid (Homoptera: Aphididae) damage on resistant and susceptible hosts with comparisons of damage ratings to quantitative plant measurements. Journal of Economic Entomology 86: 974–980.Google Scholar
Burd, JD, Porter, DR, Puterka, GJ, Haley, SD and Peairs, FB (2006) Biotypic variation among north American Russian wheat aphid (Homoptera: Aphididae) populations. Journal of Economic Entomology 99: 1862–1866.CrossRefGoogle ScholarPubMed
Catalán, P and Olmstead, RG (2000) Phylogenetic reconstruction of the genus Brachypodium P. Beauv. (Poaceae) from combined sequences of chloroplast ndhF gene and nuclear ITS. Plant Systematics and Evolution 220: 1–19.Google Scholar
Catalán, P, Shi, Y, Amstrong, L, Draper, J and Stace, CA (1995) Molecular phylogeny of the grass genus Brachypodium P. Beauv based on RFLP and RAPD analysis. Botanical Journal of the Linnean Society 117: 263–280.Google Scholar
Cho, YG, Temnykh, IT, Chen, X, Lipovich, L, McCouch, SR, Park, WD, Ayers, N and Cartinhour, S (2000) Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.). Theoretical and Applied Genetics 100: 713–722.CrossRefGoogle Scholar
Cordeiro, GM, Casu, R, McIntyre, CL, Manners, JM and Henry, RJ (2001) Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to sorghum. Plant Science 160: 1115–1123.CrossRefGoogle ScholarPubMed
Draper, J, Mur, LAJ, Jenkins, G, Ghosh-Biswas, GC, Bablak, P, Hasterok, R and Routledge, APM (2001) Brachypodium distachyon, a new model system for functional genomics in grasses. Plant Physiology 127: 1539–1555.Google Scholar
Eujayl, I, Sorrells, ME, Baum, M, Wolters, P and Powell, W (2002) Isolation of EST derived microsatellite markers for genotyping the A and B genomes of wheat. Theoretical and Applied Genetics 104: 399–407.Google Scholar
Felsenstein, J (1989) PHYLIP – Phylogeny Inference Package (Version 3.2). Cladistics 5: 164–166.Google Scholar
Garvin, DF, Gu, YQ, Hasterok, R, Hazen, SP, Jenkins, G, Mockler, TC, Mur, LAJ and Vogel, JP (2008) Development of genetic and genomic research resources for Brachypodium distachyon, a new model system for grass crop research. Crop Science 48: S69–S84.CrossRefGoogle Scholar
Gaut, BS (2002) Evolutionary dynamics of grass genomes. New Phytologist 154: 15–28.Google Scholar
Gupta, PK and Varshney, RK (2000) Development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113: 163–185.Google Scholar
Hasterok, R, Draper, J and Jenkins, G (2004) Laying the cytotaxonomic foundations of a new model grass, Brachypodium distachyon (L.) Beauv. Chromosome Research 12: 397–403.Google Scholar
Hasterok, R, Marasek, A, Donnison, IS, Armstead, I, Thomas, A, King, IP, Wolny, E, Idziak, D, Draper, J and Jenkins, G (2006) Alignment of the genomes of Brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization. Genetics 173: 349–362.Google Scholar
Hsaio, C, Chatterton, NJ, Asay, KH and Jensen, KB (1994) Molecular phylogeny of the Pooideae based on nuclear rDNA (ITS) sequences. Theoretical Applied Genetics 90: 389–398.Google Scholar
Huo, N, Gu, YQ, Lazo, GR, Vogel, JP, Coleman-Derr, D, Luo, MC, Thilmony, R, Garvin, DF and Anderson, OD (2006) Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics. Genome 49: 1099–1108.CrossRefGoogle ScholarPubMed
Huo, NX, Lazo, GR, Vogel, JP, You, FM, Ma, YQ, Hayden, DM, Coleman-Derr, D, Hill, TA, Dvorak, J, Anderson, OD, Luo, MC and Gu, YQ (2008) The nuclear genome of Brachypodium distachyon: analysis of BAC end sequences. Functional & Integrative Genomics 8: 135–147.Google Scholar
La Rota, M, Kantety, RV, Yu, J-K and Sorrells, ME (2005) Nonrandom distribution and frequencies of genomic and EST-derived microsatellite markers in rice, wheat, and barley. BMC Genomics 6: 23.Google Scholar
Metzgar, D, Bytof, J and Wills, C (2000) Selection against frameshift mutations limits microsatellite expansion in coding DNA. Genome Research 10: 72–80.Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Research 8: 4321–4325.CrossRefGoogle Scholar
Nei, M and Li, WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76: 5269–5273.CrossRefGoogle ScholarPubMed
Nicot, N, Chiquet, V, Gandon, B, Amilhat, L, Legeai, F, Leroy, P, Bernard, M and Sourdille, P (2004) Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theoretical and Applied Genetics 109: 800–805.CrossRefGoogle ScholarPubMed
Peng, JH and Lapitan, NL (2005) Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers. Functional & Integrative Genomics 5: 80–96.CrossRefGoogle ScholarPubMed
Puterka, GJ, Burd, JD, Porter, D, Shufran, K, Baker, C, Bowling, B and Patrick, C (2007) Distribution and diversity of Russian wheat aphid (Hemiptera: Aphididae) biotypes in North America. Journal of Economic Entomology 100: 1679–1684.CrossRefGoogle ScholarPubMed
Routledge, APM, Shelley, G, Smith, JV, Talbot, NJ, Draper, J and Mur, LAJ (2004) Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa). Molecular Plant Pathology 5: 253–265.Google Scholar
Saitou, N and Nei, M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.Google Scholar
Shi, Y, Draper, J and Stace, CA (1993) Ribosomal DNA variation and its phylogenetic implication in the genus Brachypodium (Poaceae). Plant Systematics and Evolution 188: 125–138.CrossRefGoogle Scholar
Thiel, T, Michalek, W, Varshney, RK and Graner, A (2003) Exploiting EST databases for the development of cDNA derived microsatellite markers in barley (Hordeum vulgare L.). Theoretical and Applied Genetics 106: 411–422.Google Scholar
Vogel, JP, Garvin, DF, Leong, OM and Hayden, DM (2006a) Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon. Plant Cell, Tissue and Organ Culture 85: 199–211.Google Scholar
Vogel, JP, Gu, YQ, Twigg, P, Lazo, GR, Chingcuanco, DL, Hayden, DM, Donze, TJ, Vivian, LA, Stamova, B and Coleman-Derr, D (2006b) EST sequencing and phylogenetic analysis of the model grass Brachypodium distachyon. Theoretical and Applied Genetics 113: 186–195.CrossRefGoogle ScholarPubMed
Weng, Y and Lazar, MD (2000) Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat. Plant Breeding 121: 218–223.Google Scholar
Weng, Y, Lazar, MD, Michels, GJ Jr and Rudd, JC (2004) Phenotypic mechanisms of host resistance against greenbug (Heteroptera: Aphididae) revealed by near isogenic lines of wheat. Journal of Economic Entomology 97: 654–660.Google Scholar
Weng, Y, Li, W, Devkota, RN and Rudd, JC (2005) Microsatellite markers associated with two Aegilops tauschii-derived greenbug resistance loci in wheat. Theoretical and Applied Genetics 110: 462–469.CrossRefGoogle ScholarPubMed
Weng, Y, Azhaguvel, P, Michels, GJ Jr and Rudd, JC (2007) Cross-species transferability of microsatellite markers from six aphid (Hemiptera: Aphididae) species and their use for evaluating biotypic diversity in two cereal aphids. Insect Molecular Biology 16: 613–622.Google Scholar
Zhang, LY, Bernard, M, Leroy, P, Feuillet, C and Sourdille, P (2005) High transferability of bread wheat EST-derived SSRs to other cereals. Theoretical and Applied Genetics 111: 677–687.CrossRefGoogle ScholarPubMed