Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-27T15:47:55.788Z Has data issue: false hasContentIssue false
  • English
  • Français

Allele mining in the gene pool of wild Solanum species for homologues of late blight resistance gene RB/Rpi-blb1

Published online by Cambridge University Press:  21 February 2011

Artem Pankin ,
Ekaterina Sokolova ,
Elena Rogozina ,
Maria Kuznetsova ,
Kenneth Deahl ,
Richard Jones  and
Emil Khavkin
Artem Pankin*
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Ekaterina Sokolova
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Elena Rogozina
Affiliation:
Institute of Plant Industry, St. Petersburg, Russia
Maria Kuznetsova
Affiliation:
Institute of Phytopathology, Bol'shiye Vyazemy, Russia
Kenneth Deahl
Affiliation:
USDA-ARS, Agriculture Research Center, Beltsville, MD, USA
Richard Jones
Affiliation:
USDA-ARS, Agriculture Research Center, Beltsville, MD, USA
Emil Khavkin
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A coiled coil-nucleotide binding site-leucine-rich repeat gene RB/Rpi-blb1 isolated from Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. RB homologues were reported in several Solanum species; some of them retained defence function. Here, we report additional evidence on RB-like sequences in 21 Solanum species of the section Petota. The panel of Solanum species was screened with three RB-related PCR markers. RB-like sequences were found in every tested Solanum accession, suggesting universal distribution of RB structural homologues among Solanum genomes, while locus-specific RB-629 was found only in 15 species. Phylogenetic analysis of RB-629 sequences suggested a highly conservative pattern of polymorphisms that was neither species- nor series-specific. Apparently, duplication and evolution of RB-like loci preceded Solanum speciation. Marker presence and particular haplotypes were not immediately associated with high LB resistance.

Keywords

late blight resistancePhytophthora infestanspotatoR genesSolanum spp.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 305 - 308
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Altschul, SF, Gish, W, Miller, W, Myers, EW and Lipman, DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.CrossRefGoogle ScholarPubMed
Champouret, N, Bouwmeester, K, Rietman, H, van der Lee, T, Maliepaard, C, Heupink, A, van de Vondervoort, PJI, Jacobsen, E, Visser, RGF, van der Vossen, EAG, Govers, F and Vleeshouwers, VGAA (2009) Phytophthora infestans isolates lacking class I IpiO variants are virulent on Rpi-blb1 potato. Molecular Plant-Microbe Interactions 22: 1535–1545.CrossRefGoogle Scholar
Colton, LM, Groza, HI, Wielgus, SM and Jiang, J (2006) Marker-assisted selection for the broad-spectrum potato late blight resistance conferred by gene RB derived from a wild potato species. Crop Science 46: 589–594.CrossRefGoogle Scholar
Dangl, JL and Jones, JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411: 826–833.CrossRefGoogle ScholarPubMed
Felsenstein, J (1989) PHYLIP – phylogeny inference package (version 3.2). Cladistics 5: 164–166.Google Scholar
Filippov, AV, Gurevich, BI, Kozlovsky, BE, Kuznetsova, MA, Rogozhin, AN, Spiglazova, SY, Smetanina, TI and Smirnov, AN (2004) A rapid method for evaluation of partial potato resistance to late blight and of aggressiveness of Phytophthora infestans isolates originating from different regions. Plant Breeding and Seed Science 50: 29–41.Google Scholar
Förch, M, van den Bosch, T, van Bekkum, P, Evenhuis, B, Vossen, J and Kessel, G (2010) Monitoring the Dutch P. infestans population for virulence against new R genes. In: Schepers, HTAM (ed.) PPO Special Report no. 14. Wageningen: DLO Foundation, pp. 45–50.Google Scholar
Fry, W (2008) Phytophthora infestans: the plant (and R gene) destroyer. Molecular Plant Pathology 9: 385–402.CrossRefGoogle Scholar
Halterman, DA, Chen, Y, Sopee, J, Berduo-Sandoval, J and Sánchez-Pérez, A (2010) Competition between Phytophthora infestans effectors leads to increased aggressiveness on plants containing broad-spectrum late blight resistance. PLoS ONE 5: e10536.CrossRefGoogle ScholarPubMed
Hawkes, JG (1990) The potato: evolution, biodiversity and genetic resources. London: Belhaven Press, 259 pp.Google Scholar
Hein, I, Birch, P, Danan, S, Lefebvre, V, Achieng Odeny, D, Gebhardt, C, Trognitz, F and Bryan, G (2009) Progress in mapping and cloning qualitative and quantitative resistance against Phytophthora infestans in potato and its wild relatives. Potato Research 52: 215–227.CrossRefGoogle Scholar
Kibbe, WA (2007) OligoCalc: an online oligonucleotide properties calculator. Nucleic Acids Research 35, (2): W43–W46.CrossRefGoogle ScholarPubMed
Liu, Z and Halterman, D (2006) Identification and characterization of RB-orthologous genes from the late blight resistant wild potato species Solanum verrucosum. Physiological and Molecular Plant Pathology 69: 230–239.CrossRefGoogle Scholar
Lokossou, AA, Rietman, H, Wang, M, Krenek, P, van der Schoot, H, Henken, B, Hoekstra, R, Vleeshouwers, VG, van der Vossen, EA, Visser, RG, Jacobsen, E and Vosman, B (2010) Diversity, distribution, and evolution of Solanum bulbocastanum late blight resistance genes. Molecular Plant-Microbe Interactions 23: 1206–1216.CrossRefGoogle ScholarPubMed
Oosumi, T, Rockhold, D, Maccree, M, Deahl, K, McCue, K and Belknap, W (2009) Gene Rpi-bt1 from Solanum bulbocastanum confers resistance to late blight in transgenic potatoes. American Journal of Potato Research 86: 456–465.CrossRefGoogle Scholar
Song, J, Bradeen, JM, Naess, SK, Raasch, JA, Wielgus, SM, Haberlach, GT, Liu, J, Kuang, H, Austin-Phillips, S, Buell, CR, Helgeson, JP and Jiang, J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences USA 100: 9128–9133.CrossRefGoogle ScholarPubMed
Spooner, DM, van den Berg, RG, Rodrigues, A, Bamberg, JB, Hijmans, RJ and Lara-Cabrera, SI (2004) Wild Potatoes (Solanum section Petota; Solanaceae) of North and Central America. Ann Arbor, MI: American Society of Plant Taxonomists.CrossRefGoogle Scholar
Tan, M, Hutten, R, Visser, R and van Eck, H (2010) The effect of pyramiding Phytophthora infestans resistance genes R Pi-mcd1 and R Pi-ber in potato. Theoretical and Applied Genetics 121: 117–125.CrossRefGoogle Scholar
van der Vossen, E, Sikkema, A, Hekkert, BL, Gros, J, Stevens, P, Muskens, M, Wouters, D, Pereira, A, Stiekema, W and Allefs, S (2003) An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. Plant Journal 36: 867–882.CrossRefGoogle ScholarPubMed
Vleeshouwers, VGAA, Rietman, H, Krenek, P, Champouret, N, Young, C, Oh, S-K, Wang, M, Bouwmeester, K, Vosman, B, Visser, RGF, Jacobsen, E, Govers, F, Kamoun, S and Vossen, EAGV (2008) Effector genomics accelerates discovery and functional profiling of potato disease resistance and Phytophthora infestans avirulence genes. PLoS ONE 3: e2875.CrossRefGoogle ScholarPubMed
Wang, M, Allefs, S, van den Berg, R, Vleeshouwers, V, van der Vossen, E and Vosman, B (2008) Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum. Theoretical and Applied Genetics 116: 933–943.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Pankin Supplementary Material

Pankin Supplementary Material

Download Pankin Supplementary Material(PDF)
PDF 48.9 KB