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Validation of QTLs associated with spikelets per panicle and grain weight in rice

Published online by Cambridge University Press:  16 July 2014

Sang-Min Yeo ,
Yeo-Tae Yun ,
Dong-Min Kim ,
Chong-Tae Chung  and
Sang-Nag Ahn
Sang-Min Yeo
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
Yeo-Tae Yun
Affiliation:
Chungnam Agricultural Research and Extension Services, Yesan 340-861, Republic of Korea
Dong-Min Kim
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
Chong-Tae Chung
Affiliation:
Chungnam Agricultural Research and Extension Services, Yesan 340-861, Republic of Korea
Sang-Nag Ahn*
Affiliation:
Department of Agronomy, Chungnam National University, Daejeon 305-764, Republic of Korea
*
* Corresponding author. E-mail: [email protected]
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Abstract

In this study, a near-isogenic line (BC4F10) CR572 developed by introgressing a chromosomal segment from Oryza rufipogon (accession no. 105491) into the Oryza sativa subsp. japonica cv. Hwaseong was found to exhibit a significant increase in the number of spikelets per panicle (SPP) and grain weight compared with the recurrent parent Hwaseong. Quantitative trait locus (QTL) analysis in F2 generation derived from the cross between CR572 and Hwaseong revealed that two QTLs, qSPP1 and qTGW1, were linked to a simple sequence repeat marker, RM283, on chromosome 1. The additive effect of the O. rufipogon allele at qSPP1 was 13 SPP, and 21.6% of the phenotypic variance was explained by the segregation of RM283. The qTGW1 QTL explained 19.1% of the phenotypic variance for grain weight. Substitution mapping was carried out with five F3 lines derived from F2 plants having informative recombination breakpoints within the target region. Substitution mapping indicated the linkage of qSPP1 and qTGW1. The grain yield of CR572 was 18.2 and 15.8% higher than that of Hwaseong at two locations, respectively, mainly due to the increase in 1000-grain weight and SPP. These results are very useful for QTL cluster transfer by molecular marker-assisted selection in rice breeding programmes and for QTL gene cloning by map-based cloning.

Keywords

1000-grain weightQTLnear-isogenic linesricespikelets per panicle
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S151 - S154
Copyright
Copyright © NIAB 2014 

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