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High-molecular-weight (HMW) glutenin subunit composition of the Elite-II synthetic hexaploid wheat subset (Triticum turgidum × Aegilops tauschii; 2n = 6x = 42; AABBDD)

Published online by Cambridge University Press:  14 November 2011

Amna Bibi ,
Awais Rasheed ,
Alvina Gul Kazi ,
Tariq Mahmood ,
Saifullah Ajmal ,
Iftikhar Ahmed  and
Abdul Mujeeb-Kazi
Amna Bibi
Affiliation:
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Awais Rasheed*
Affiliation:
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Alvina Gul Kazi
Affiliation:
National University of Science and Technology (NUST), Islamabad, Pakistan
Tariq Mahmood
Affiliation:
Plant Biochemistry and Molecular Biology Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Saifullah Ajmal
Affiliation:
Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan
Iftikhar Ahmed
Affiliation:
National Agriculture Research Center (NARC), Islamabad, Pakistan
Abdul Mujeeb-Kazi
Affiliation:
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan
*
*Corresponding author. E-mail: [email protected]
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Abstract

Characterization of high-molecular-weight (HMW) glutenins is an important criterion for identifying genotypes with good bread-making quality. In synthetic hexaploids (SHs), the D-genome encodes several allelic variants of HMW glutenins that require proper identification prior to their utilization for bread wheat (BW) improvement. In this study, SHs with promising agronomic features were characterized for HMW glutenin composition. Seven different allelic variants were observed at the Glu-Dt1 locus, three of which (1Dx1.5+1Dy10, 1Dx1.5+1Dy12.2 and 1Dx2.1+1Dy10) have not been previously reported in existing BW germplasm. The results also showed a variety of D-genome-encoded subunits along with superior glutenin alleles in the B-genome (1Bx7+1By8, 1Bx6+1By8 and 1Bx13+1By16). About 63% of these SHs encoded favourable allelic variants of HMW glutenins, which make them a good choice for improvement in wheat bread making. Glu-Dt1 encoded favourable allelic variants (1Dx5+1Dy10 and 1Dx1.5+1Dy10) that are frequently observed in SHs can be easily incorporated into BW through recombination breeding.

Keywords

Aegilops tauschiiGlu-1 lociHMW glutenin subunitssynthetic hexaploids
Type
Short Communication
Information
Plant Genetic Resources , Volume 10 , Issue 1 , April 2012 , pp. 1 - 4
Copyright
Copyright © NIAB 2011

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