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Genetic stability of cryopreserved ornamental Lilium germplasm

Published online by Cambridge University Press:  04 May 2022

Jae-young Song Open the ORCID record for Jae-young Song [Opens in a new window] ,
Jung-yoon Yi ,
Jinjoo Bae ,
Jung-ro Lee ,
Mun-sup Yoon  and
Young-yi Lee
Jae-young Song
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jung-yoon Yi
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jinjoo Bae
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Jung-ro Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Mun-sup Yoon
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
Young-yi Lee*
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju, 54874, Republic of Korea
*
Author for correspondence: Young-yi Lee, E-mail: [email protected]
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Abstract

The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars (L. bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety (L. callosum var. flavum) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. The results suggest a very low level or the absence of genetic variation in terms of morphological and genetic stability among the plants regenerated after cryopreservation.

Keywords

CryopreservationGenetic stabilityISSR
Type
Short Communication
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 66 - 68
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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