Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-15T07:30:00.322Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of mango landraces from Mexico based on molecular markers

Published online by Cambridge University Press:  03 April 2009

Didiana Gálvez-López ,
Sanjuana Hernández-Delgado ,
Maurilio González-Paz ,
Enrique Noe Becerra-Leor ,
Miguel Salvador-Figueroa  and
Netzahualcoyotl Mayek-Pérez
Didiana Gálvez-López
Affiliation:
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Blvd. del Maestro esquina Elías Piña s/n, Col. Narciso Mendoza, Reynosa, Tamaulipas88710, Mexico
Sanjuana Hernández-Delgado
Affiliation:
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Blvd. del Maestro esquina Elías Piña s/n, Col. Narciso Mendoza, Reynosa, Tamaulipas88710, Mexico
Maurilio González-Paz
Affiliation:
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Blvd. del Maestro esquina Elías Piña s/n, Col. Narciso Mendoza, Reynosa, Tamaulipas88710, Mexico
Enrique Noe Becerra-Leor
Affiliation:
Campo Experimental Cotaxtla, Instituto Nacional de Investigaciones Forestales Agropecuarias (INIFAP), Apdo. Postal 429, Veracruz, Mexico
Miguel Salvador-Figueroa
Affiliation:
Área de Biotecnología, Universidad Autónoma de Chiapas, Carretera a Puerto Madero Km. 2.0, Tapachula, Chiapas30700, Mexico
Netzahualcoyotl Mayek-Pérez*
Affiliation:
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Blvd. del Maestro esquina Elías Piña s/n, Col. Narciso Mendoza, Reynosa, Tamaulipas88710, Mexico
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Genetic diversity and relationships among 112 mango (Mangifera indica) plants native to 16 states of Mexico and four controls [three mango cultivars (Ataulfo, Manila and Tommy Atkins) and one accession of Mangifera odorata] were evaluated based on amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) molecular markers. Mango germplasm shows broad dispersion through Mexico and genetically similar germplasm from different agroecological regions has previously been found by our group. Both AFLP and SSR analyses indicated high genetic similarity among mango populations that were clustered in two major groups: mangos from Gulf of Mexico coastline and mangos from Pacific Ocean coastline and locations far away from the sea. The highest genetic diversity was found within plants from each state, and significant genetic differentiation (FST = 0.1921, AFLPs and 0.1911, SSRs) was also observed among mango populations based on geographical origin and genetic status (cultivars versus landraces). Heterozygosity values ranged from low (0.38) to moderate (0.68), and no heterozygote deficits were found. The highest genetic variability was found in mango populations from Tabasco, Michoacán and Oaxaca. Data suggested that mangoes are subjected to natural or induced pollination, so segregation as well as genetic recombination plays major roles on genetic diversification of Mexican mangos. AFLP analysis was more robust than SSR for determining the genetic relationships among mango landraces from Mexico.

Keywords

AFLP markersdiversitygermplasmMangifera indica L. Mangifera odorata L.SSR markers
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 3 , December 2009 , pp. 244 - 251
Copyright
Copyright © NIAB 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barboza, SVA and da Costa, LPS (2004) Genetic variability of mango germplasm genotypes detected by RAPD markers. Acta Horticulturae 645: 303–310.Google Scholar
Chávez, CX, Veja, AP, Tapia, LM and Miranda, S (2001) Mango, su manejo y producción en el trópico seco de México. INIFAP, Morelia, México, pp. 17–25.Google Scholar
Degani, C, El-Batsri, R and Gazit, S (1990) Enzyme polymorphism in mango. Journal of the American Society for Horticultural Science 115: 844–847.CrossRefGoogle Scholar
Degani, C, Cohen, M, El-Batsri, R and Gazit, S (1992) PGI isozyme diversity and its genetic control in mango. HortScience 27: 252–254.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin 19: 11–15.Google Scholar
Duval, MF, Bunel, J, Sitbon, C and Risterucci, M (2005) Development of microsatellite markers for mango (Mangifera indica L.). Molecular Ecology Notes 5: 824–826.CrossRefGoogle Scholar
Duval, ME, Bunel, J, Sitbon, C, Risterucci, AM, Calabre, C and Le Bellec, F (2006) Genetic diversity of Caribbean mangoes (Mangifera indica L.) using microsatellite markers. 8th International Mango Symposium, Sun City, South Africa.Google Scholar
Eiadthong, W, Yonemori, K, Kanzaki, S, Sugiura, A, Utsunomiya, N and Subhadrabandhu, S (2000) Amplified fragment length polymorphism analysis for studying genetic relationships among Mangifera species in Thailand. Journal of the American Society for Horticultural Science 125: 160–164.CrossRefGoogle Scholar
Excoffier, L, Laval, G and Schneider, S (2005) Arlequin Version 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47–50.Google Scholar
Felsenstein, J (2004) Inferring Phylogenies. Sunderland, MA: Sinauer Associates, pp. 335–363.Google Scholar
Felsenstein, J (2006) PHYLIP (Phylogeny Inference Package) Version 3.6 distributed by the author, Department of Genome Sciences, University of Washington, Seattle, Washington. Available online only at http://evolution.genetics.washington.edu/phylip/software.html (accessed 27 May 2006).Google Scholar
Gálvez-López, D, Adriano-Anaya, ML, Villarreal-Treviño, C, Mayek-Pérez, N and Salvador-Figueroa, M (2007) Diversidad isoenzimática de mangos criollos del sur de Chiapas, México. Revista Chapingo, Serie Horticultura 13: 71–76.CrossRefGoogle Scholar
Hernández-Delgado, S, Becerra-Leor, EN, González-Paz, M and Mayek-Pérez, N (2005) Diversidad genética en germoplasma de mango (Mangifera sp.) con marcadores moleculares AFLP 17° Encuentro Nacional de Investigación Científica y Tecnológica del Golfo de México, Altamira, México.Google Scholar
Honsho, C, Nishiyama, K, Eiadthong, W and Yonemori, K (2005) Isolation and characterization of new microsatellite markers in mango (Mangifera indica). Molecular Ecology Notes 5: 152–154.CrossRefGoogle Scholar
Huff, DR, Peakall, R and Smouse, PE (1993) RAPD variation within and among natural populations of outcrossing buffalograss [(Buchöe dayloides (Nutt.) Engelm]. Theoretical and Applied Genetics 86: 927–934.CrossRefGoogle Scholar
López-Valenzuela, JA, Martínez, O and Paredes-López, O (1997) Geographic differentiation and embryo type identification in Mangifera indica L. cultivars using RAPD markers. HortScience 32: 1105–1108.CrossRefGoogle Scholar
Marshall, TC, Slate, J, Kruuk, L and Pemberton, JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology 7: 639–655.CrossRefGoogle ScholarPubMed
Moritz, C (1994) Applications of mitochondrial DNA analysis in conservation: a critical review. Molecular Ecology 3: 401–411.CrossRefGoogle Scholar
Mueller, U and Wolfenbarger, L (1999) AFLP genotyping and fingerprinting. Tree 14: 389–394.Google ScholarPubMed
Nei, M and Li, W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76: 5256–5273.CrossRefGoogle ScholarPubMed
Pandit, SS, Mitra, S, Giri, AP, Pujari, KH, Patil, BP, Jambhale, ND and Gupta, VS (2007) Genetic diversity analysis of mango cultivars using inter simple sequence repeat markers. Current Science 93: 1135–1141.Google Scholar
Perrière, G and Gouy, M (1996) WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78: 364–369.CrossRefGoogle ScholarPubMed
Ramos, NJA (2003) Perspectivas de la Red Mango para el 2003. FIRA-Banco de México, México, pp. 2–12.Google Scholar
Riek, J, Calsyn, D, Everaert, E, Van, B and Loose, MD (2001) AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theoretical and Applied Genetics 103: 1254–1265.CrossRefGoogle Scholar
Rivera-Ocasio, E, Aide, TM and McMillan, O (2002) Patterns of genetic diversity and biogeographical history of the tropical wetland tree Pterocarpus officinalis (Jacq.), in the Caribbean basin. Molecular Ecology 11: 675–677.CrossRefGoogle ScholarPubMed
Schneider, S, Kueffer, JM, Roessli, D and Excoffier, L (1997) Arlequin Version 1.1: a software for population genetic data analysis. Genetics and Biometry Laboratory, University of Geneva, Geneva, Switzerland.Google Scholar
Schnell, RJ, Olano, CT, Quintanilla, WE and Meerow, W (2005) Isolation and characterization of 15 microsatellite mango (Mangifera indica L.) and cross-species amplification in closely related taxa. Molecular Ecology Notes 5: 625–627.CrossRefGoogle Scholar
Sun, G, Díaz, O, Salomón, B and Bothmer, R (1999) Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers. Genome 42: 420–431.CrossRefGoogle ScholarPubMed
Vos, P, Hogers, R, Bleeker, M, Reijans, M, van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.CrossRefGoogle ScholarPubMed
Supplementary material: File

Lopez supplementary material

Table.doc

Download Lopez supplementary material(File)
File 33.3 KB