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First report of genome size and ploidy of the underutilized leguminous tuber crop Yam Bean (Pachyrhizus erosus and P. tuberosus) by flow cytometry

Published online by Cambridge University Press:  22 May 2019

Kalidas Pati ,
Fangning Zhang  and
Jacqueline Batley
Kalidas Pati*
Affiliation:
ICAR-Central Tuber Crops Research Institute, Regional Centre, Bhubaneswar, Odisha 751019, India School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
Fangning Zhang
Affiliation:
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
Jacqueline Batley
Affiliation:
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
*
*Corresponding author. E-mail: [email protected]
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Abstract

Cytological understanding is an important parameter to understand the genetic architecture of yam bean. The ploidy level and genome size of two cultivated species of yam bean (Pachyrhizus erosus and P. tuberosus) were estimated using flow cytometric analysis of young leaf tissue, with propidium iodide as a fluorescent dye. Six genotypes of P. erosus and three genotypes of P. tuberosus were analysed. Rice (Oryza sativa cv. Nipponbare) and Mung bean (Vigna radiata cv Berken) were used as internal reference standards. Variation of 2C nuclear DNA content among the six P. erousus lines was 4.18%, ranging from 1.17 to 1.22 pg, whereas only 1.8% variation was observed among the three P. tuberosus lines, which ranged from 1.07 to 1.09 pg. Moreover, it was found that the nuclear DNA content of P. tuberosus was lower than that of P. erosus. The result of the flow cytometric analysis showed that all the species were diploid (2n = 2x) and coefficient of variation (CV%) of all the accessions of the two species was less than 3.5%. This is the first report of ploidy analysis and genome size estimation of the leguminous underutilized tuber crop yam bean using flow cytometry. This result will be helpful for yam bean genome sequencing and crop improvement programmes.

Keywords

DNA contentflow cytometrygenome sizePachyrhizus sppploidy levelunderutilized crop
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 5 , October 2019 , pp. 456 - 459
Copyright
Copyright © NIAB 2019 

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References

Ammiraju, JS, Luo, M, Goicoechea, JL, Wang, W, Kudrna, D, Mueller, C, Talag, J, Kim, H, Sisneros, NB and Blackmon, B (2006) The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza. Genome Research 16: 140–147.Google Scholar
Arumuganathan, K and Earle, E (1991) Estimation of nuclear DNA content of plants by flow cytometry. Plant Molecular Biology Reporter 9: 229–241.Google Scholar
Doležel, J, Binarová, P and Lcretti, S (1989) Analysis of nuclear DNA content in plant cells by flow cytometry. Biologia Plantarum 31: 113–120.Google Scholar
Doležel, J, Greilhuber, J and Suda, J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols 2: 2233–2244.Google Scholar
Draper, J, Mur, LA, Jenkins, G, Ghosh-Biswas, GC, Bablak, P, Hasterok, R and Routledge, AP (2001) Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiology 127: 1539–1555.Google Scholar
Galbraith, DW, Harkins, KR, Maddox, JM, Ayres, NM, Sharma, DP and Firoozabady, E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051.Google Scholar
Gosálvez, M (1983) Carcinogenesis with the insecticide rotenone. Life Sciences 32: 809–816.Google Scholar
Greilhuber, J and Obermayer, R (1997) Genome size and maturity group in Glycine max (soybean). Heredity 78: 547–551.Google Scholar
Greilhuber, J and Obermayer, R (1998) Genome size variation in Cajanus cajan (Fabaceae): a reconsideration. Plant Systematics and Evolution 212: 135–141.Google Scholar
Ingham, JL (1990) Systematic aspects of phytoalexin formation within tribe Phaseoleae of the Leguminosae (subfamily Papilionoideae). Biochemical Systematics and Ecology 18: 329–343.Google Scholar
Juarez, M and Paredes-López, O (1994) Studies on jicama juice processing. Plant Foods for Human Nutrition (Formerly Qualitas Plantarum) 46: 127–131.Google Scholar
Kang, YJ, Satyawan, D, Shim, S, Lee, T, Lee, J, Hwang, WJ, Kim, SK, Lestari, P, Laosatit, K and Kim, KH (2015) Draft genome sequence of adzuki bean, Vigna angularis. Scientific Reports 5: 8069.Google Scholar
Naganowska, B, Wolko, B, Śliwińska, E and Kaczmarek, Z (2003) Nuclear DNA content variation and species relationships in the genus Lupinus (Fabaceae). Annals of Botany 92: 349–355.Google Scholar
Santayana, M, Rossel, G, Núñez, J, Sørensen, M, Delêtre, M, Robles, R, Fernández, V, Grüneberg, WJ and Heider, B (2014) Molecular characterization of cultivated species of the genus Pachyrhizus Rich. ex DC. by AFLP markers: calling for more data. Tropical Plant Biology 7: 121–132.Google Scholar
Sato, S, Nakamura, Y, Kaneko, T, Asamizu, E, Kato, T, Nakao, M, Sasamoto, S, Watanabe, A, Ono, A and Kawashima, K (2008) Genome structure of the legume, Lotus japonicus. DNA Research 15: 227–239.Google Scholar
Schmutz, J, Cannon, SB, Schlueter, J, Ma, J, Mitros, T, Nelson, W, Hyten, DL, Song, Q, Thelen, JJ and Cheng, J (2010) Genome sequence of the palaeopolyploid soybean. Nature 463: 178.Google Scholar
Sørensen, M (1996) Yam Bean: Pachyrhizus DC.-Promoting the Conservation and use of Underutilized and Neglected Crops. 2. Rome: Bioversity International.Google Scholar
Suda, J and Leitch, IJ (2010) The quest for suitable reference standards in genome size research. Cytometry Part A 77: 717–720.Google Scholar
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