Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-24T19:11:30.008Z Has data issue: false hasContentIssue false
  • English
  • Français

Determining genetic combining ability, heterotic potential and gene action for yield contributing traits and Yellow Vein Mosaic Virus (YVMV) resistance in Okra (Abelmoschus esculentus (L.) Monech.)

Published online by Cambridge University Press:  27 October 2020

Anjan Das Open the ORCID record for Anjan Das [Opens in a new window] ,
Ramesh Kumar Yadav ,
Harshawardhan Choudhary ,
Saurabh Singh Open the ORCID record for Saurabh Singh [Opens in a new window] ,
Yogesh P. Khade  and
Rahul Chandel
Anjan Das*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Ramesh Kumar Yadav
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Harshawardhan Choudhary
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Saurabh Singh
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Yogesh P. Khade
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune-410505, India
Rahul Chandel
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Breeding for resistance to biotic stress and higher yield is a continuous process. Thus, the identification of desirable parents with good combining ability and nature of gene action for the target trait is of utmost importance. Hence, in this present investigation, 10 lines and three testers of Okra were crossed in line × tester mating design to generate 30 testcross progenies and their evaluation along with parents and check in a randomized complete block design with three replications. To depict the true picture of genetic variation among the parental genotypes, molecular diversity analysis was also carried out using genomic-simple sequence repeats before crossing to ascertain that sufficient variability is present among the parents. The molecular analysis grouped the parental genotypes into four clusters (I–IV). The analysis of variance revealed that all the treatments were significant for most of the traits. The combining ability analysis suggested Pusa A-4 as the best general combiner for earliness, Pusa Bhindi-5 for high yield, and DOV-92 for fruit length, plant height, yield per plant, and coefficient of infection for Yellow Vein Mosaic Virus Disease resistance. Similarly, the specific combining ability analysis suggested that the cross combinations DOV-92 × Pusa Bhindi-5 followed by DOV-92 × Pusa A-4 and DOV-92 × Pusa Sawani exhibit high economic heterosis for yield per plant as well as for disease resistance. Finally, estimation of the degree of dominance and predictability ratio was also worked out which indicated the prevalence of non-additive gene action for most of the traits pointing towards sufficient scope for heterosis breeding in Okra.

Keywords

combining abilitygenetic diversityheritabilityheterosisOkrasimple sequence repeatsvirus resistance
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 5 , October 2020 , pp. 316 - 329
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aditika, KH, Priyanka, SS and Singh, S (2020) Heterotic potential, potence ratio, combining ability and genetic control of quality and yield traits in bell pepper under net-house conditions of NW Himalayas. Agricultural Research 16: 1–10.Google Scholar
Bag, MK, Gautam, NK, Prasad, TV, Pandey, S, Dutta, M and Roy, A (2014) Evaluation of an Indian collection of black gram germplasm and identification of resistance sources to mungbean yellow mosaic virus. Crop Protection 61: 92–101.10.1016/j.cropro.2014.03.021CrossRefGoogle Scholar
Bruce, AB (1910) The Mendelian theory of heredity and the augmentation of vigor. Science 32(827): 627–628.10.1126/science.32.827.627-aCrossRefGoogle ScholarPubMed
Dahake, KD and Bangar, ND (2006) Combining ability analysis in okra. Journal of Maharashtra Agricultural Universities 31: 39–41.Google Scholar
Davenport, CB (1908) Degeneration, albinism and inbreeding. Science 28(718): 454–455.10.1126/science.28.718.454-bCrossRefGoogle ScholarPubMed
Desale, CS, Mehta, DR and Singh, AP (2014) Combining ability analysis in bread wheat. Journal of Wheat Research 6: 330–339.Google Scholar
East, EM (1908) Inbreeding in corn. In: Reports of the Connecticut Agricultural Experiment Station for Years 1907–1908, pp. 419–428.Google Scholar
FAOSTAT (2014) Available online at: http://faostat.fao.org/ (Accessed February 8, 2017).Google Scholar
Fasahat, P, Rajabi, A, Rad, MJ and Derera, J (2016) Principles and utilization of combining ability in plant breeding. Biometrics & Biostatistics International Journal 4: 1–24.10.15406/bbij.2016.04.00085CrossRefGoogle Scholar
Ghosh, J, Ghosh, SK, Chattarjee, H and Senapati, SK (1999) Pest constraints of okra under terai region of West Bengal. Indian Journal of Entomology 61: 362–371.Google Scholar
Gupta, SK, Nepolean, T, Shaikh, CG, Rai, K, Hash, CT, Das, RR and Rathore, A (2018) Phenotypic and molecular diversity-based prediction of heterosis in pearl millet [Pennisetum glaucum L. (R.) Br.]. The Crop Journal 6: 271–281.CrossRefGoogle Scholar
Jaiprakashnarayan, RP, Prashanth, SJ, Mulge, R and Madalageri, MB (2008) Study on heterosis and combining ability for earliness and yield parameters in okra [Abelmoschus esculentus (L.) Moench]. The Asian Journal of Horticulture 3: 136–141.Google Scholar
Jindal, SK, Deepak, A and Ghai, TR (2009) Heterobeltiosis and combining ability for earliness in okra [Abelmoschus esculentus (L.) Moench]. Journal of Crop Improvement 36: 59–66.Google Scholar
Kempthorne, O (1957) An Introduction to Genetic Statistics. New York: John Wiley and Sons, Inc..Google Scholar
Khatik, KR, Chaudhary, R and Khatik, CL (2013) General and specific combining ability effects for yield and its component in okra [Abelmoschus esculentus (L.) Moench]. International Journal of Agricultural Sciences 9: 80–83.Google Scholar
Kumar, S, Sokona, D, Adamou, H, Alain, R, Dov, P and Christophe, K (2010) Okra (Abelmoschus Spp.) in West and Central Africa: potential and progress on its improvement. African Journal of Agricultural Research 5: 3590–3598.Google Scholar
Kumar, P, Singh, V and Dubey, RK (2012) Combining ability analysis in okra [Abelmoschus esculentus (L.) Moench]. Vegetable Science 39: 180–182.Google Scholar
Kumar, S, Parekh, MJ, Fougat, RS, Patel, SK, Patel, CB, Kumar, M and Patel, BR (2016) Assessment of genetic diversity among okra genotypes using SSR markers. Journal of Plant Biochemistry and Biotechnology 26: 172–178.CrossRefGoogle Scholar
Medagam, TR, Kadiyala, H, Mutyala, G and Hameedunnisa, B (2012) Heterosis for yield and yield components in okra [Abelmoschus esculentus (L.) Moench]. Chilean Journal of Agricultural Research 72: 316.CrossRefGoogle Scholar
Mihretu, Y, Wayessa, G and Adugna, D (2014) Multivariate analysis among okra [Abelmoschus esculentus (L.) Moench] collection in South Western Ethiopia. Journal of Plant Sciences 9: 43–50.Google Scholar
Munshi, AD and Verma, VK (1999) Combining ability in muskmelon (Cucumis melo). Indian Journal of Agricultural Sciences 69: 214–216.Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–4326.CrossRefGoogle ScholarPubMed
Perrier, X and Jacquemoud-Collet, JP (2006) DARwin software. Available at http://darwin.cirad.fr/.Google Scholar
Reddy, AM, Sridevi, O, Salimath, PM, Nadaf, HL, Patil, MS and Hosamani, RM (2013) Heterosis for yield and yield components in okra. International Journal of Advanced Research 1: 287–302.Google Scholar
Schafleitner, R, Kumar, S, Lin, CY, Hegde, SG and Ebert, A (2013) The Okra (Abelmoschus esculentus) transcriptome as a source for gene sequence information and molecular markers for diversity analysis. Gene 517: 27–36.10.1016/j.gene.2012.12.098CrossRefGoogle ScholarPubMed
Shetty, AA, Singh, JP and Singh, D (2013) Resistance to yellow vein mosaic virus in okra: a review. Biological Agriculture and Horticulture 29: 159–164.10.1080/01448765.2013.793165CrossRefGoogle Scholar
Shull, GH (1908) The composition of a field of maize. Journal of Heredity 1: 296–301.CrossRefGoogle Scholar
Singh, D (1973) Diallel analysis over environments-I. Indian Journal of Genetics and Plant Breeding 33: 127–136.Google Scholar
Singh, S, Bhatia, R, Kumar, R, Sharma, K, Dash, S and Dey, SS (2018) Cytoplasmic male sterile and doubled haploid lines with desirable combining ability enhances the concentration of important antioxidant attributes in Brassica oleracea. Euphytica 214: 207.CrossRefGoogle Scholar
Singh, S, Dey, SS, Bhatia, R, Kumar, R, Sharma, K and Behera, TK (2019) Heterosis and combining ability in cytoplasmic male sterile and doubled haploid based Brassica oleracea progenies and prediction of heterosis using microsatellites. PloS One 14: e0210772.10.1371/journal.pone.0210772CrossRefGoogle ScholarPubMed
Solankey, SS, Singh, AK and Singh, RK (2013) Genetic expression of heterosis for yield and quality traits during different growing seasons in okra (Abelmoschus esculentus). Indian Journal of Agricultural Sciences 83: 815–819.Google Scholar
Solankey, SS, Singh, AK and Singh, RK (2016) Heterosis of okra resistance sources for okra yellow vein mosaic virus (OYVMV) in okra (Abelmoschus esculentus). Indian Journal of Agricultural Sciences 86: 1460–1465.Google Scholar
Sprague, GF and Tatum, LA (1942) General vs Specific combining ability in single cross of corn. Journal of American Society of Agronomy 34: 923–932.CrossRefGoogle Scholar
Vijayaraghavan, C and Warrier, VA (1946) Evaluation of high yielding hybrids in bhendi (Hibischus esculentus). Proceedings of 33rd Indian Science Congress, Bangalore, India. pp: 163.Google Scholar
Wassimi, NN, Isleib, TG and Hosfield, GL (1986) Fixed effect genetic analysis of a diallel cross in dry beans (Phaseolus vulgaris L.). Theoritical and Applied Genetics 72: 449–454.10.1007/BF00289525CrossRefGoogle Scholar
Wynne, JC, Emery, DA and Rice, PM (1970) Combining ability estimates in Arachis Hypogeae L. II. Field performance of F1 hybrids. Crop Science 10: 713–715.10.2135/cropsci1970.0011183X001000060036xCrossRefGoogle Scholar
Supplementary material: File

Das et al. supplementary material

Tables S1-S9

Download Das et al. supplementary material(File)
File 49.8 KB