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Correction of the misclassification of species in the Portuguese collection of Cucurbita pepo L. using DNA markers

Published online by Cambridge University Press:  16 July 2014

Ricardo Rodrigues ,
Iris Veiga ,
António Marreiros ,
Filomena Rocha  and
José Leitão
Ricardo Rodrigues
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
Iris Veiga
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
António Marreiros
Affiliation:
Direção Regional de Agricultura e Pescas do Algarve (DRAALG), Apartado 282, Patacão, 8001-904Faro, Portugal
Filomena Rocha
Affiliation:
Banco Português de Germoplasma Vegetal (BPGV)/INIAV,I.P., Quinta de S. José, S. Pedro de Merelim, 4700-859Braga, Portugal
José Leitão*
Affiliation:
Laboratory of Genomics and Genetic Improvement, FCT, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
*
* Corresponding author. E-mail: [email protected]
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Abstract

In this study, the genetic variability among 130 accessions of the Portuguese germplasm collection of Cucurbita pepo L. maintained at the Banco Português de Germoplasma Vegetal was assessed using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques for the identification of a genetically diverse core group of accessions for field phenotypic analysis. The surprisingly completely different molecular patterns exhibited by multiple accessions was later confirmed in the distribution of the putative C. pepo plants into two clusters drastically separated at a very low level of genetic similarity (DICE coefficient = 0.37). Additional analyses with RAPD and ISSR (inter single sequence repeat) markers and the introduction of standard genotypes of C. maxima L. and C. moschata L. into the analyses allowed the identification of multiple accessions of the last species wrongly included in the C. pepo collection. This study is a good example of the usefulness of DNA markers in the establishment and management of plant germplasm collections.

Keywords

CucurbitaceaeCucurbita pepoDNA markersplant genetic resources
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S160 - S163
Copyright
Copyright © NIAB 2014 

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References

Cabrita, LF, Aksoy, U, Hepaksoy, S and Leitão, JM (2001) Suitability of isozyme, RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones. Scientia Horticulturae 87: 261–273.Google Scholar
Carlier, J, Leitão, J and Fonseca, F (2008) Population genetic structure of Cistus ladanifer L. (Cistaceae) and genetic differentiation from co-occurring Cistus species. Plant Species Biology 23: 141–151.Google Scholar
Farinhó, M, Coelho, P, Carlier, J, Svetleva, D, Monteiro, A and Leitão, J (2004) Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica). Theoretical and Applied Genetics 109: 1392–1398.Google Scholar
Ferriol, M, Picó, B and Nuez, F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics 107: 271–282.Google Scholar
Ferriol, M, Pico, B and Nuez, F (2004) Morphological and molecular diversity of a collection of Cucurbita maxima landraces. Journal of the American Society for Horticultural Science 129: 60–69.Google Scholar
Gong, L, Paris, HS, Nee, MH, Stift, G, Pachner, M, Vollmann, J and Lelley, T (2012) Genetic relationships and evolution in Cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms. Theoretical and Applied Genetics 124: 875–891.Google Scholar
Goulão, L, Cabrita, L, Oliveira, CM and Leitão, JM (2001) Comparing RAPD and AFLPTM analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars. Euphytica 119: 259–270.Google Scholar
Gwanama, C, Labuschagne, MT and Botha, AM (2000) Analysis of genetic variation in Cucurbita moschata by random amplified polymorphic DNA (RAPD) markers. Euphytica 113: 19–24.Google Scholar
Monte-Corvo, L, Cabrita, L, Oliveira, C and Leitão, J (2000) Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers. Genetic Resources and Crop Evolution 47: 257–265.Google Scholar
Sikdar, B, Bhattacharya, M, Mukherjee, A, Banerjee, A, Ghosh, E, Ghosh, B and Roy, SC (2010) Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers. Biologia Plantarum 54: 135–140.Google Scholar
Wu, J, Chang, Z, Wu, Q, Zhan, H and Xie, S (2011) Molecular diversity of Chinese Cucurbita moschata germplasm collections detected by AFLP markers. Scientia Horticulturae 128: 7–13.Google Scholar
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