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Assessment of genetic diversity within and among sage (Salvia) species using SRAP markers

Published online by Cambridge University Press:  01 December 2015

Zahra Aghaei ,
Majid Talebi  and
Mehdi Rahimmalek
Zahra Aghaei
Affiliation:
Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan84156-83111, Iran
Majid Talebi*
Affiliation:
Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan84156-83111, Iran
Mehdi Rahimmalek
Affiliation:
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan84156-83111, Iran
*
*Corresponding author. E-mail: [email protected]
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Abstract

Salvia (sage) is the most important and largest genus of the Lamiaceae family. High similarities among species in this genus lead to difficulty in its systematic identification. Despite its economic importance, limited molecular studies have been conducted to evaluate the genetic diversity among and within Salvia species. In this study, SRAP (sequence-related amplified polymorphism) markers, which targeted ORFs (open reading frames) as functional regions in the genome, were used to detect the genetic diversity of five Salvia species (S. virgata Jacq., S. nemorosa L., S. officinalis L., S. cereal L. and S. sclarea L.). Fourteen primer combinations (PCs) were amplified by 265 fragments on 54 genotypes, in which 255 (96%) were polymorphic. The average polymorphism information content (PIC) value was 0.308 over all PCs. The genetic distance among species ranged from 0.126 (between S. virgata Jacq. and S. nemorosa L.) to 0.568 (between S. nemorosa L. and S. sclarea L.). Based on Jaccard's similarity coefficient and UPGMA algorithm, cluster analysis separated different species (r= 0.920). The results showed high genetic differentiation (Fst= 0.337) and negligible gene flow (Nm= 0.750) among species. Owing to the high genetic variation among and within Salvia species, it serves as a rich source of germplasm with potential for use in breeding programmes.

Keywords

AMOVAgenetic variationSalviaSRAP
Type
Short Communications
Information
Plant Genetic Resources , Volume 15 , Issue 3 , June 2017 , pp. 279 - 282
Copyright
Copyright © NIAB 2015 

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References

Bassam, BJ, Caetano-Anolles, G and Gresshoff, PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196: 80–83.Google Scholar
Bertea, CM, Azzolin, CMM, Bossi, S, Doglia, G and Maffei, M (2005) Identification of an EcoRI restriction site for a rapid and precise determination of β-asarone-free Acorus calamus cytotypes. Phytochemistry 66: 507–514.Google Scholar
Delamare, A, Pistorello, I, Artico, L, Serafini, LA and Echverrigaray, S (2007) Antibacterial activity of essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chemistry 100: 603–608.Google Scholar
Gali-Muhtasib, H, Hilan, C and Khater, C (2000) Traditional uses of Salvia libanotica (East Mediterranean sage) and the effects of its essential oils. Journal of Ethnopharmacology 71: 513–520.Google Scholar
Karaca, M, Saha, S, Callahan, FE, Jenkins, JN, Read, JJ and Percy, RG (2004) Molecular and cytological characterization of a cytoplasmic-specific mutant in pima cotton (Gossypium barbadense L.). Euphytica 139: 187–197.Google Scholar
Karaca, M, Ince, AG, Tugrul-Ay, S, Turgut, K and Onus, AN (2008) PCR-RFLP and DAMD-PCR genotyping for Salvia species. Journal of the Science of Food and Agriculture 88: 2508–2516.Google Scholar
Kelen, M and Tepe, B (2008) Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology 99: 4096–4104.CrossRefGoogle ScholarPubMed
Li, G and Quiros, C (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica . Theoretical and Applied Genetics 103: 455–461.Google Scholar
Mader, E, Lohwasser, U, Börner, A and Novak, J (2010) Population structures of genebank accessions of Salvia officinalis L. (Lamiaceae) revealed by high resolution melting analysis. Biochemical Systematics and Ecology 38: 178–186.Google Scholar
Peng, L, Ru, M, Wang, B, Wang, Y, Li, B, Yu, J and Liang, Z (2014) Genetic diversity assessment of germplasm collection of Salvia miltiorrhiza Bunge. based on morphology, ISSR and SRAP markers. Biochemical Systematics and Ecology 55: 84–92.Google Scholar
Radosavljević, I, Jakse, J, Javornik, B, Satovic, Z and Liber, Z (2011) New microsatellite markers for Salvia officinalis (Lamiaceae) and cross-amplification in closely related species. American Journal of Botany 98: e316–e318.Google Scholar
Radosavljević, I, Satovic, Z, Jakse, J, Javornik, B, Greguras, D, Jug-Dujakovic, M and Liber, Z (2012) Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas. International Journal of Molecular Sciences 13: 12082–12093.CrossRefGoogle ScholarPubMed
Reales, A, Rivera, D, Palazon, JA and Obon, C (2004) Numerical taxonomy study of Salvia sect. Salvia (Labiatae). Botanical Journal of the Linnean Society 145: 353–371.Google Scholar
Rohlf, FJ (1998) NTSYS-PC Numerical Taxonomy and Multivariate Analysis System, Version 2.02. Setauket, NY: Exeter Publications, pp. 1–31.Google Scholar
Sepehry Javan, Z, Rahmani, F and Heidari, R (2012) Assessment of genetic variation of genus Salvia by RAPD and ISSR markers. Australian Journal of Crop Sciences 6: 1068–1073.Google Scholar
Skoula, M, El-Hilali, I and Makris, A (1999) Evaluation of the genetic diversity of Salvia fruticosa Mill. clones using RAPD markers and comparison with the essential oil profiles. Biochemical Systematics and Ecology 27: 559–568.Google Scholar
Smith, JSC, Chin, ECL, Shu, H, Smith, OS, Wall, SJ, Senior, ML, Mitchell, SE, Kresovich, S and Zeigle, J (1997) An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparison with data from RFLPs and pedigree. Theoretical and Applied Genetics 95: 163–173.Google Scholar
Song, Z, Li, X, Wang, X and Wang, H (2010) Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica 138: 241–249.Google Scholar
Walker, JB and Sytsma, KJ (2007) Staminal evolution in the genus Salvia (Lamiaceae): molecular phylogenetic evidence for multiple origins of the staminal lever. Annals of Botany 100: 375–391.Google Scholar
Walker, JB, Sytsma, KJ, Treutlein, J and Wink, M (2004) Salvia is not monophyletic: implications for the systematics radiation and ecological specializations of Salvia and tribe Mentheae. American Journal of Botany 91: 1115–1125.Google Scholar
Wang, X, Zhou, X, Gao, W, Cui, G, Huang, L and Lui, C (2011) New analysis of EST-SSR distribution and development EST-SSR markers in Salvia miltiorrhiza . Zhongguo Zhongyao Zazhi 36: 289–293.Google Scholar
Yeh, FC and Yang, RC (1999) POPGENE Version 1.31. Edmonton, Alberta: University of Alberta and Tim Boyle, Center for International Forestry Research.Google Scholar
Zhang, Y, Li, X and Wang, Z (2013) Diversity evaluation of Salvia miltiorrhiza using ISSR markers. Biochemical Genetics 51: 707–721.Google Scholar
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