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Assessment of diversity in tropical soybean (Glycine max (L.) Merr.) varieties and elite breeding lines using single nucleotide polymorphism markers

Published online by Cambridge University Press:  17 February 2021

Abush Tesfaye Abebe Open the ORCID record for Abush Tesfaye Abebe [Opens in a new window] ,
Adesike Oladoyin Kolawole ,
Nnanna Unachukwu ,
Godfree Chigeza ,
Hailu Tefera  and
Melaku Gedil
Abush Tesfaye Abebe
Affiliation:
International Institute of Tropical Agriculture, Ibadan, Nigeria
Adesike Oladoyin Kolawole
Affiliation:
Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Nnanna Unachukwu
Affiliation:
International Institute of Tropical Agriculture, Ibadan, Nigeria
Godfree Chigeza
Affiliation:
International Institute of Tropical Agriculture, Lusaka, Zambia
Hailu Tefera
Affiliation:
Private Consultant, 2384 Rolling Fork Circle, #403, Herndon, VA20171, USA
Melaku Gedil*
Affiliation:
International Institute of Tropical Agriculture, Ibadan, Nigeria
*
*Corresponding author. E-mail: [email protected]
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Abstract

Soybean (Glycine max (L.) Merr.) is an important legume crop with high commercial value widely cultivated globally. Thus, the genetic characterization of the existing soybean germplasm will provide useful information for enhanced conservation, improvement and future utilization. This study aimed to assess the extent of genetic diversity of soybean elite breeding lines and varieties developed by the soybean breeding programme of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. The genetic diversity of 65 soybean genotypes was studied using single-nucleotide polymorphism (SNP) markers. The result revealed that 2446 alleles were detected, and the indicators for allelic richness and diversity had good differentiating power in assessing the diversity of the genotypes. The three complementary approaches used in the study grouped the germplasm into three major clusters based on genetic relatedness. The analysis of molecular variance revealed that 71% (P < 0.001) variation was due to among individual genotypes, while 11% (P < 0.001) was ascribed to differences among the three clusters, and the fixation index (FST) was 0.11 for the SNP loci, signifying moderate genetic differentiation among the genotypes. The identified private alleles indicate that the soybean germplasm contains diverse variability that is yet to be exploited. The SNP markers revealed high diversity in the studied germplasm and found to be efficient for assessing genetic diversity in the crop. These results provide valuable information that might be utilized for assessing the genetic variability of soybean and other legume crops germplasm by breeding programmes.

Keywords

allelic diversitybreeding programmesgenetic relatednessSNPsoybean germplasm
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 1 , February 2021 , pp. 20 - 28
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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