Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-28T08:03:40.709Z Has data issue: false hasContentIssue false
  • English
  • Français

Vaccination of young lambs by means of a protein fraction extracted from adult Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

E. A. Munn ,
C. A. Greenwood  and
W. J. Coadwell
E. A. Munn
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
C. A. Greenwood
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
W. J. Coadwell
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
Get access Rights & Permissions [Opens in a new window]

Summary

Lambs aged 48–150 days which had been injected with extracts enriched in a functional antigen (contortin) obtained from adult Haemonchus contortus developed specific circulating antibodies and were less susceptible to haemonchosis when challenged 1 month later with a single dose of 20000–25000 infective 3rd-stage larvae. Sera from 18 out of a total of 19 lambs injected with the extract contained precipitating antibodies to 2–5 components of the extract. None of these lambs died. The one extract-injected lamb which did not develop antibodies and 9 of the 13 lambs used as controls died of haemonchosis. The average weight of worms recovered was 1·45 g from the immune lambs and 5·72 g from the non-immune lambs.

Type
Research Article
Information
Parasitology , Volume 94 , Issue 2 , April 1987 , pp. 385 - 397
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alger, N. E. (1968). Haemonchus contortus: somatic and metabolic antigens of third and fourth larval and adult stages. Experimental Parasitology 23, 187–97.CrossRefGoogle ScholarPubMed
Allonby, E. W. & Dargie, J. D. (1974). Ovine haemonchosis. In Helminth Diseases of Cattle, Sheep and Horses in Europe (ed. Urquhart, G. M. and Armour, J.), pp. 59–71. Glasgow: The University Press.Google Scholar
Baermann, G. (1917). Eine einfache Method zur Auffindung von Anchylostomum (Nemotoden) Larven in Erdproben. Geneeskundig Tijdschrift voor Nederlandsch-Indie 57, 131–7.Google Scholar
Clegg, J. A. & Smith, M. A. (1978). Prospects for the development of dead vaccines against helminths. Advances in Parasitology 16, 165–218.CrossRefGoogle ScholarPubMed
Coadwell, W. J. & Ward, P. F. V. (1975). Observations on the development of Haemonchus contortus in young sheep given a single infection. Parasitology 71, 505–15.CrossRefGoogle Scholar
Coadwell, W. J. & Ward, P. F. V. (1977). Annual variation in the growth of Haemonchus contortus in experimental infections of sheep and its relation to arrested development. Parasitology 74, 121–32.CrossRefGoogle Scholar
Coadwell, W. J. & Ward, P. F. V. (1981). The development, composition and maintenance of experimental populations of Haemonchus contortus in sheep. Parasitology 82, 257–61.CrossRefGoogle ScholarPubMed
Coadwell, W. J. & Ward, P. F. V. (1982). The use of faecal egg counts for estimating worm burdens in sheep infected with Haemonchus contortus. Parasitology 85, 251–6.CrossRefGoogle ScholarPubMed
Colglazier, M. L., Kates, K. C. & Enzie, F. D. (1974). Cambendazole-resistant Haemonchus contortus strain in sheep: further experimental development. Journal of Parasitology 60, 289–92.CrossRefGoogle ScholarPubMed
Drudge, J. H., Leland, S. E. Jr & Wyant, Z. N. (1957). Strain variation in the response of sheep nematodes to the action of Phenothiazine. I. Studies of mixed infections in experimental animals. American Journal of Veterinary Research 18, 133–41.Google Scholar
Drudge, J. H., Szanto, J., Wyant, Z. N. & Elan, G. (1964). Field studies on parasite control in sheep: comparison of Thiabendazole, Ruelene and Phenothiazine. American Journal of Veterinary Research 25, 1512–18.Google ScholarPubMed
Gordon, H. Mc. L. & Whitlock, H. V. (1939). A new technique for counting nematode eggs in sheep faeces. Joint Council for Scientific and Industrial Research 12, 50–2.Google Scholar
Henney, R. W., Maclean, J. M. & Mulligan, W. (1971). The effect of host immunity on the metabolism of Nippostrongylus brasiliensis. Immunology 21, 711–18.Google Scholar
Jarrett, W. F. H., Jennings, F. W., MacIntyre, W. I. M., Mulligan, W. & Sharp, N. C. C. (1959). Studies on immunity to Haemonchus contortus infection – vaccination of sheep using a single dose of X-irradiated larvae. American Journal of Veterinary Research 20, 527–31.Google Scholar
Lee, D. L. (1969). Changes in adult Nippostrongylus brasiliensis during the development of immunity to this nematode in rats. 1. Changes in ultrastructure. Parasitology 59, 29–39.CrossRefGoogle ScholarPubMed
Lee, D. L. & Martin, J. (1980). The structure of the intestine of Nematodirus battus and changes during the course of an infection in lambs. Parasitology 81, 27–33.CrossRefGoogle ScholarPubMed
Lopez, V. & Urquhart, G. M. (1967). The immune response of merino sheep to Haemonchus contortus infection. 3rd International Conference of the World Association for the Advance of Veterinary Parasitology, pp. 153–9.Google Scholar
Love, R. J., Ogilvie, B. M. & McLaren, D. J. (1976). The immune mechanism which expels the intestinal stage of Trichinella spiralis from rats. Immunology 30, 7–15.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Miller, G. L. (1959). Protein determination for large numbers of samples. Analytical Chemistry 31, 964.CrossRefGoogle Scholar
Mulligan, W., Gordon, H. McL., Stewart, D. F. & Wagland, B. M. (1961). The use of irradiated larvae as immunizing agents in Haemonchus contortus and Trichostrongylus colubriformis infections of sheep. Australian Journal of Agricultural Research 12, 1176–87.CrossRefGoogle Scholar
Munn, E. A. (1976). The structure of the extracellular polymeric protein, contortin, and its mode of attachment to the plasma membrane. Proceedings of the VIth European Congress of Electron Microscopy, Jerusalem, pp 515–16.Google Scholar
Munn, E. A. (1977). A helical, polymeric extracellular protein associated with the luminal surface of Haemonchus contortus intestinal cells. Tissue and Cell 9, 23–34.CrossRefGoogle ScholarPubMed
Murray, M. (1973). Local immunity and its role in vaccination. Veterinary Record 91, 500–4.CrossRefGoogle Scholar
Neilson, J. T. M. (1975). Failure to vaccinate lambs against Haemonchus contortus with functional metabolic antigens identified by Immunoelectrophoresis. International Journal for Parasitology 5, 427–30.CrossRefGoogle ScholarPubMed
Ogilvie, B. M. & Hockley, D. J. (1968). Effects of immunity on Nippostrongylus brasiliensis adult worms: reversible and irreversible changes in infectivity, reproduction, and morphology. Journal of Parasitology 54, 1073–84.CrossRefGoogle ScholarPubMed
Ozerol, N. H. & Silverman, P. H. (1970). Further characterization of active metabolites from histotropic larvae of Haemonchus contortus cultured in vitro. Journal of Parasitology 46, 1119–205.Google Scholar
Pery, P. & Luffau, G. (1979). Antigens of helminths. In The Antigens, vol. 5 (ed. Sela, M.), pp. 142–172. London: Academic Press.Google Scholar
Seesee, F. M., Wescott, R. B. & Garham, J. R. (1976). Nippostrongylus brasiliensis: Indirect fluorescent antibody studies of immunity in mice. Experimental Parasitology 39, 214–21.CrossRefGoogle ScholarPubMed
Smith, W. D. (1977 a). Serum and mucus antibodies in sheep immunised with larval antigens of Haemonchus contortus. Research in Veterinary Science 22, 128–9.CrossRefGoogle ScholarPubMed
Smith, W. D. (1977 b). Anti-larval antibodies in the serum and abomasal mucus of sheep hyperinfected with Haemonchus contortus. Research in Veterinary Science 22, 334–8.CrossRefGoogle ScholarPubMed
Soulsby, E. J. L., Sommerville, R. I. & Stewart, D. F. (1959). Antigenic stimulus of exsheathing fluid in selfcure of sheep infested with Haemonchus contortus. Nature, London 183, 553–4.CrossRefGoogle ScholarPubMed
Soulsby, E. J. L. & Stewart, D. F. (1960). Serological studies of the self-cure reaction in sheep infected with Haemonchus contortus. Australian Journal of Agricultural Research 11, 595–603.CrossRefGoogle Scholar
Symons, L. E. A. & Steele, J. W. (1978). Pathogenesis of the loss of production in gastrointestinal parasitism. In The Epidemiology and Control of Gastrointestinal Parasites of Sheep in Australia (ed. Donald, A. D., Southcott, W. H. and Dineen, J. K.), pp. 9–22. East Melbourne, Victoria: CSIRO Publications.Google Scholar
Turton, J. A. & Clarke, C. J. (1974). The effect of natural worm loss on the estimate of anthelmintic activity in an anthelmintic test with Haemonchus contortus. Parasitology 69, 191–6.CrossRefGoogle Scholar
Urquhart, G. M., Jarrett, W. F. H. & Mulligan, W. (1962). Helminth immunity. Advances in Veterinary Science 7, 87–146.Google Scholar
Urquhart, G. M., Jarrett, W. F. H., Jennings, F. W., McIntyre, W. I. M., Mulligan, W. & Sharp, N. C. C. (1966). Immunity to Haemonchus contortus infection: failure of X-irradiated larvae to immunize young lambs. American Journal of Veterinary Research 27, 1641–3.Google ScholarPubMed
Urquhart, G. M., Jarrett, W. F. H., Jennings, F. W., McIntyre, W. I. M. & Mulligan, W. (1966). Immunity to Haemonchus contortus infection: relationship between age and successful vaccination with irradiated larvae. American Journal of Veterinary Research 27, 1645–8.Google ScholarPubMed