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Use of two-colour flow cytometry to assess killing of Giardia muris trophozoites by antibody and complement

Published online by Cambridge University Press:  06 April 2009

M. F. Heyworth  and
J. Pappo
M. F. Heyworth
Affiliation:
Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA
J. Pappo
Affiliation:
Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA
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Summary

A two-colour flow cytometry technique was used to assess killing of Giardia muris trophozoites by rabbit anti-trophozoite antibodies and complement. Binding of rabbit antibody to trophozoites and killing of trophozoites were documented by flow cytometry, after incubating the organisms with fluorescein-conjugated anti-rabbit IgG and propidium iodide (PI). Percentages of PI+ (dead) trophozoites ranged from >80%, after incubation with rabbit antiserum and complement, to <30%, after incubation with complement alone. The assay technique may be applicable to studies aimed at determining whether intestinal antibodies from Giardia-infected mammals can kill Giardia trophozoites.

Keywords

Giardia murisflow cytometrytrophozoiteskillingcytotoxic antibodycomplement
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 199 - 203
Copyright
Copyright © Cambridge University Press 1989

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