Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-28T15:59:06.213Z Has data issue: false hasContentIssue false
  • English
  • Français

The use of plasma etching to reveal the internal structure of Nippostrongylus brasiliensis (Nematoda)

Published online by Cambridge University Press:  06 April 2009

D. L. Lee  and
C. D. Nicholls
D. L. Lee
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
C. D. Nicholls
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
Get access Rights & Permissions [Opens in a new window]

Summary

Plasma etching has been used to strip away the cortical layers of the cuticle of adult Nippostrongylus brasiliensis to reveal the struts, with their supporting fibres, which are found in the fluid-filled middle layer of the cuticle, and the basal fibre layers. The etched specimens were studied by means of scanning electron microscopy. The results support earlier work, obtained by transmission electron microscopy, on the cuticle of this nematode. Plasma etching has been shown to have potential in studying the structure of nematodes.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 3 , June 1983 , pp. 477 - 480
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Hollahan, J. R. (1974). Applications of plasmas to chemical and physical analysis. In Techniques and Applications of Plasma Chemistry (ed. Hollahan, J. R. and Bell, A. T.), pp. 229–253. New York: Wiley-Interscience.Google Scholar
Jamuar, M. (1966). Electron microscope studies on the body wall of the nematode Nippostrongylus brasiliensis. Journal of Parasitology 52, 209–32.CrossRefGoogle Scholar
Lee, D. L. (1965). The cuticle of adult Nippostrongylus brasiliensis. Parasitology 55, 173–81.CrossRefGoogle ScholarPubMed
Lee, D. L. (1970). Moulting in nematodes: the formation of the adult cuticle during the final moult of Nippostrongylus brasiliensis. Tissue and Cell 2, 139–53.CrossRefGoogle ScholarPubMed
Lucker, J. T. (1936). Preparasitie moults of Nippostrongylus muris with remarks on the structure of the cuticula of Triehostrongyles. Parasitology 28, 161–71.CrossRefGoogle Scholar
Sharpe, M. J. & Lee, D. L. (1981). Observations on the structure and function of the haemoglobin from the cuticle of Nippostrongylus brasiliensis (Nematoda). Parasitology 83, 411–24.CrossRefGoogle Scholar
Thomas, R. S. (1969). Microincineration techniques for electron microscopic localisation of biological minerals. Advances in Optical and Electron Microscopy 3, 99–154.Google Scholar
Yokogawa, S. (1922). The development of Heligmosum muris Yokogawa, a nematode from the intestine of the wild rat. Parasitology 14, 127–66.CrossRefGoogle Scholar