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Use of fluorescent probes as a useful tool to identify resistant Schistosoma mansoni isolates to praziquantel

Published online by Cambridge University Press:  21 June 2010

F. F. B. COUTO ,
P. M. Z. COELHO ,
N. ARAÚJO ,
J. R. KUSEL ,
N. KATZ  and
A. C. A. MATTOS
F. F. B. COUTO
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
P. M. Z. COELHO*
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
N. ARAÚJO
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
J. R. KUSEL
Affiliation:
Division of Infection and Immunity, IBLS, University of Glasgow, Scotland, UK
N. KATZ
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
A. C. A. MATTOS*
Affiliation:
Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil
*
*Corresponding authors: Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil. Tel: +55 31 3349 7740/ +55 31 3349 7759. Fax: +5531 3295 3115. E-mail: [email protected]/[email protected]
*Corresponding authors: Laboratório de Esquistossomose, Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, MG, Brasil. Tel: +55 31 3349 7740/ +55 31 3349 7759. Fax: +5531 3295 3115. E-mail: [email protected]/[email protected]
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Summary

The use of chemotherapy on a mass scale in endemic areas may lead to the appearance of resistant isolates through the mechanism of selective drug pressure. Studies have demonstrated that praziquantel (PZQ) is able to inhibit the excretory activity and to cause tegumental damage in Schistosoma mansoni adult worms. The use of the probe resorufin to evaluate excretory activity, as well as the probe Hoechst 33258 to detect tegumental damage in adult worms, may represent a method to identify resistant (or less susceptible) isolates. The purpose of the present work was to compare the changes caused by PZQ in the function of the excretory system and in the integrity of the tegument of adult worms from the LE isolate (susceptible to PZQ) and the LE-PZQ isolate (less susceptible to PZQ). Worms from the isolate LE-PZQ showed less severe tegumental lesions, in both in vitro and in vivo experiments, detected by labelling with Hoechst 33258 and continued to have a functional excretory system as shown by labelling with resorufin in vitro.

Keywords

Schistosoma mansonidrug resistancepraziquantelfluorescent probes
Type
Research Article
Information
Parasitology , Volume 137 , Issue 12 , October 2010 , pp. 1791 - 1797
Copyright
Copyright © Cambridge University Press 2010

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