Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-05T08:29:13.045Z Has data issue: false hasContentIssue false

Use of differential display to detect changes in gene expression in the intermediate snail host Biomphalaria glabrata upon infection with Schistosoma mansoni

Published online by Cambridge University Press:  01 April 2000

A. E. LOCKYER
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
C. S. JONES
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
L. R. NOBLE
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
D. ROLLINSON
Affiliation:
Biomedical Parasitology Division, Zoology Department, Natural History Museum, London SW7 5BD

Abstract

Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata. Down regulation of one 241 bp mRNA expressed sequence fragment – with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues – has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.

Type
Research Article
Copyright
2000 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)