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Unravelling the phylogenetic relationships of African trypanosomes of suids

Published online by Cambridge University Press:  12 July 2001

W. C. GIBSON ,
J. R. STEVENS ,
C. M. T. MWENDIA ,
J. N. MAKUMI ,
J. M. NGOTHO  and
J. M. NDUNG'U
W. C. GIBSON
Affiliation:
School of Biological Sciences, University of Bristol, Bristol BS8 1UG
J. R. STEVENS
Affiliation:
School of Biological Sciences, University of Exeter, Exeter EX4 4PS
C. M. T. MWENDIA
Affiliation:
Kenya Trypanosomiasis Research Institute, PO Box 362, Kikuyu, Kenya
J. N. MAKUMI
Affiliation:
Kenya Trypanosomiasis Research Institute, PO Box 362, Kikuyu, Kenya
J. M. NGOTHO
Affiliation:
Kenya Trypanosomiasis Research Institute, PO Box 362, Kikuyu, Kenya
J. M. NDUNG'U
Affiliation:
Kenya Trypanosomiasis Research Institute, PO Box 362, Kikuyu, Kenya
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Abstract

African trypanosomes of the subgenera Nannomonas and Pycnomonas have been recorded from both wild and domestic suids. However, complete descriptions of some of these trypanosomes with regard to host range, pathogenicity, transmission and distribution are still lacking. Neither the recently described Trypanosoma (Nannomonas) godfreyi nor Trypanosoma (Nannomonas) congolense Tsavo have been isolated from mammalian hosts, while Trypanosoma (Pycnomonas) suis remains the rarest of the Salivarian trypanosomes. The only isolate presumed to be of the latter species is maintained at the Kenya Trypanosomiasis Research Institute, Nairobi. We present here the results of characterization of this isolate by morphology, tsetse transmission, the use of species-specific DNA probes and DNA sequence analysis. Morphology in stained blood smears revealed a small trypanosome with a free flagellum. Experimental transmission through Glossina morsitans morsitans showed a developmental cycle typical of subgenus Nannomonas. A positive identification was obtained with species-specific PCR primers for T. congolense Tsavo; moreover, the sequence of the SSU rRNA gene was almost identical to that of T. congolense Tsavo on database. In phylogenetic analysis of the SSU rRNA genes of Salivarian trypanosomes, T. congolense Tsavo grouped with T. simiae rather than T. congolense, suggesting that the name T. simiae Tsavo is more appropriate.

Keywords

NannomonasTrypanosoma suisTrypanosoma simiaetsetseDNA probephylogenetics
Type
Research Article
Information
Parasitology , Volume 122 , Issue 6 , June 2001 , pp. 625 - 631
Copyright
© 2001 Cambridge University Press

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