Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-17T19:45:54.308Z Has data issue: false hasContentIssue false
  • English
  • Français

The ultrastructural localization of Echinococcus granulosus antigen 5

Published online by Cambridge University Press:  06 April 2009

M. K. Jones ,
L.-H. Zhang ,
G. R. Leggatt ,
D. J. Stenzel  and
D. P. McManus
M. K. Jones*
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
L.-H. Zhang
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
G. R. Leggatt
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
D. J. Stenzel
Affiliation:
Analytical Electron Microscopy Facility, Queensland University of Technology, Brisbane, Qld 4001, Australia
D. P. McManus
Affiliation:
Tropical Health Program, Queensland Institute of Medical Research, 300 Herston Road, Herston, Qld 4029, Australia
*
* Corresponding author: Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Qld 4072, Australia. Tel: +61 7 3365 3249. Fax: +61 7 3365 2199. E-mail: [email protected].
Get access Rights & Permissions [Opens in a new window]

Summary

Murine monoclonal and polyclonal antisera, raised against the 38 kDa subunit of Echinococcus granulosus antigen 5, were used to investigate the tissue distribution of the antigen in hydatid cysts. Immunoreactivity was visualized by indirect immunofluorescence on whole protoscoleces, and ultrastructural immunocytochemistry utilizing colloidal gold-based labelling procedures on unsectioned and cryosectioned brood capsules and protoscoleces. In protoscoleces, the 38 kDa subunit of antigen 5 was localized at the interface of parenchymal cells and associated extracellular matrices, as well as along the interface of the tegumentary syncytium in the somal region and its basal matrix. Cytoplasmic labelling of parenchymal cells was rare; when observed, it was associated with vesicles and membranes in cytoplasmic extensions of parenchymal cells. In brood capsules, the antigen was associated with the external face of the plasma of degenerating parenchymal cells. The 38 kDa subunit occurs along the extracellular face of cell membranes, suggesting that antigen 5 is either a component of the membranes or associated extracellular matrices.

Keywords

Echinococcus granulosushydatid cystimmunocytochemistryantigen 5
Type
Research Article
Information
Parasitology , Volume 113 , Issue 3 , September 1996 , pp. 213 - 222
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bènichou, J. C., Frèhel, C. & Ryter, A. (1990). Improved sectioning and ultrastructure of bacteria and animal cells embedded in Lowicryl. Journal of Electron Microscopy Technique 14, 289–297.Google Scholar
Conn, D. B. (1988). The role of cellular parenchyma and extracellular matrix in the histogenesis of the paruterine organ of Mesocestoides lineatus (Platyhelminthes: Cestoda). Journal of Morphology 197, 303–314.CrossRefGoogle ScholarPubMed
Conn, D. B. (1993). The biology of flatworms (Platyhelminthes): parenchyma cells and extracellular matrices. Transactions of the American Microscopical Society 112, 241–261.Google Scholar
Davies, C., Rickard, M. D., Bout, D. T. & Smyth, J. D. (1978). Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules of Echinococcus granulosus and E. multilocularis. Parasitology 77, 143–152.CrossRefGoogle ScholarPubMed
Griffiths, G. (1993). Fine Structure Immunocytochemistry. Springer Verlag, Berlin.Google Scholar
Leggatt, G. R. & McManus, D. P. (1994). Identification and diagnostic value of a major antibody epitope on the 12 kDa antigen from Echinococcus granulosus (hydatid disease) cyst fluid. Parasite Immunology 16, 87–96.Google Scholar
Lightowlers, M. W. & Gottstein, B. (1995). Echinococcosis/hydatidosis: antigen, immunological and molecular diagnosis. In Echinococcus and Hydatid Disease (ed. Thompson, R. C. A. & Lymbery, A. J.) pp. 355–410. CAB International, Wallingford, UK.Google Scholar
Liou, W. & Slot, J. W. (1994). Improved fine structure in immunolabelled cryosections after modifying the sectioning and pick-up conditions. Proceedings of the 13th International Congress of Electron Microscopy 3A, 253–254.Google Scholar
March, F., Enrich, C., Sánchez, F., Muñoz, C., Coll, P. & Prats, G. (1991). Echinococcus granulosus: antigen characterization by chemical treatment and enzymatic deglycosylation. Experimental Parasitology 73, 433–439.Google Scholar
Martin, R. M., Gasser, R. B., Jones, M. K. & Lightowlers, M. W. (1995). Identification and characterization of myophilin, a muscle specific antigen of Echinococcus granulosus. Molecular and Biochemical Parasitology 70, 139–148.CrossRefGoogle ScholarPubMed
McManus, D. P. & Barrett, N. J. (1985). Isolation, fractionation and partial characterization of the tegumental surface from protoscoleces of the hydatid organism, Echinococcus granulosus. Parasitology 90, 111–129.CrossRefGoogle ScholarPubMed
Morseth, D. J. (1967). Fine structure of the hydatid cyst and protoscolex of Echinococcus granulosus. Journal of Parasitology 53, 312–325.Google Scholar
Oriol, R., Williams, J. F., Perez Esandi, M. V. & Oriol, C. (1971). Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. American Journal of Tropical Medicine and Hygiene 20, 569–574.Google Scholar
Richards, K. S., Arme, C. & Bridges, J. F. (1984). Echinococcus granulosus equinus: variation in the germinal layer of murine hydatids and evidence of autophagy. Parasitology 89, 35–47.CrossRefGoogle ScholarPubMed
Rickard, M. D., Davies, C., Bout, D. T. & Smyth, J. D. (1977). Immunohistological localization of two hydatid antigens (antigen 5 and antigen B) in the cyst wall, brood capsules and protoscoleces of Echinococcus granulosus (ovine and equine) and E. multilocularis using immunoperoxidase methods. Journal of Helminthology 51, 359–364.Google Scholar
Rickard, M. D. & Lightowlers, M. W. (1986). Immunodiagnosis of hydatid disease. In The Biology of Echinococcus and Hydatid Disease, (ed. Thompson, R. C. A.) pp. 217–249. George Allen & Unwin, London.Google Scholar
Rogan, M. T. & Richards, K. S. (1989). Development of the tegument of Echinococcus granulosus (Cestoda) protoscoleces during cystic differentiation in vivo. Parasitology Research 75, 299–306.CrossRefGoogle ScholarPubMed
Sánchez, F., Garcia, J., March, F., Cardeñosa, N., Coll, P., Muñoz, C., Auladell, C. & Prats, G. (1993). Ultrastructural localization of major hydatid fluid antigens in brood capsules and protoscoleces of Echinococcus granulosus of human origin. Parasite Immunology 15, 441–447.Google Scholar
Sánchez, F., March, F., Mercader, M., Coll, P., Muñoz, C. & Prats, G. (1991). Immunocytochemical localization of major hydatid fluid antigens in protoscoleces and cysts of Echinococcus granulosus from human origin. Parasite Immunology 13, 583–592.Google Scholar
Singh, G. & Lee, R. E. (1979). Ultrastructure of hydatid cyst. Archives of Pathology and Laboratory Medicine 103, 459–462.Google Scholar
Shepherd, J. C. (1988). Antigens for the immunodiagnosis of hydatid disease. Ph.D. thesis, Imperial College of Science and Technology, London.Google Scholar
Shepherd, J. C. & McManus, D. P. (1987). Specific and cross-reactive antigens of Echinococcus granulosus hydatid cyst fluid. Molecular and Biochemical Parasitology 25, 143–154.Google Scholar
Yarzabal, L., Dupas, H., Bout, D. & Capron, A. (1976). Echinococcus granulosus: Distribution of hydatid fluid antigens in tissues of the larval stage. I. Localization of the specific antigen of hydatid fluid (Antigen 5). Experimental Parasitology 40, 391–396.CrossRefGoogle Scholar
Zhang, L-H., Leggatt, G. L. & McManus, D. P. (1995). Further characterization of the 38 kDa antigen from Echinococcus granulosus (hydatid disease) cyst fluid evidence for antigenic heterogeneity and reactivity with anti-P1 antibodies. Parasite Immunology 17, 287–296.Google Scholar