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Ultrastructural localization of an Echinococcus granulosus laminin-binding protein

Published online by Cambridge University Press:  01 March 1999

M. K. JONES ,
L.-H. ZHANG ,
R. J. GOULD  and
D. P. McMANUS
M. K. JONES
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia Molecular Parasitology Unit, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Queensland 4006, Australia
L.-H. ZHANG
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Queensland 4006, Australia
R. J. GOULD
Affiliation:
Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
D. P. McMANUS
Affiliation:
Molecular Parasitology Unit, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Queensland 4006, Australia
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Abstract

Murine antibodies, raised against a purified recombinant 30 kDa laminin-binding protein from Echinococcus granulosus, were used to investigate the tissue distribution of the native protein in protoscoleces and brood capsules. Immunofluorescence, in combination with confocal microscopy, revealed that the protein was distributed in small annular foci near the peripheral regions of the protoscoleces. Immunoelectron microscopy of thawed cryosections demonstrated that the laminin-binding protein was present in the cytoplasm of tegumentary cytons and myocytons, but not in cells of the excretory system. The protein was associated with amorphous regions of the cytoplasm, and was not expressed at the surfaces of cells. This distribution resembles those of other invertebrate laminin-binding proteins, which are thought to act in the cell cycle and cell proliferation events. A low degree of label was consistently detected in extracellular matrices of the protoscolex.

Keywords

CestodaEchinococcus granulosuslamininlaminin-binding proteinribosomal proteinimmunolocalizationultrastructuretegument
Type
Research Article
Information
Parasitology , Volume 118 , Issue 3 , March 1999 , pp. 319 - 325
Copyright
1999 Cambridge University Press

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