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Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis

Published online by Cambridge University Press:  06 April 2009

Caroline Davies ,
M. D. Rickard ,
D. T. Bout  and
J. P. Smyth
Caroline Davies
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
M. D. Rickard
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
D. T. Bout
Affiliation:
Services d'Immunologie et de Biologie Parasitaire de la Faculté de Médecine et de l'Institut Pasteur, Lille
J. P. Smyth
Affiliation:
Department of Zoology and Applied Entomology, Imperial College, London SW7
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Summary

The unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid–Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable.

Type
Research Article
Information
Parasitology , Volume 77 , Issue 2 , October 1978 , pp. 143 - 152
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Bortoletti, G. & Ferretti, G. (1973). Investigation on larval forms of Echinococcus granulosus with electron microscope. Rivista di Parassitologia 34, 89–110.Google Scholar
Bout, D., Fruit, J. & Capron, A. (1974). Purification d'un antigène spécifique de liquide hydatique Annales d'Immunologie 125, 775–88.Google Scholar
Capron, A., Vernes, A. & Biguet, J. (1967). Le diagnostic immunoelectrophorétique de l'hydatidose. In Le Kyste Hydatique du Foie (ed. S.I.M.E.P.), pp. 27–40. Journées Lyonnaises d'Hydatidologie, Lyon.Google Scholar
Erlandsen, S. L., Parsons, J. A. & Taylor, T. D. (1974). Ultrastructural immunocytochemical localization of lysozyme in the paneth cells of man. Journal of Histochemistry and Cytochemistry 22, 401–13.CrossRefGoogle ScholarPubMed
Morseth, D. J. (1967). Fine structure of the hydatid cyst and protoscolex of Echinococcus granulosus. Journal of Parasitology 53, 312–25.CrossRefGoogle ScholarPubMed
Oriol, C. & Oriol, R. (1975). Physicochemical properties of lipoprotein antigen of Echinococcus granulosus. American Journal of Tropical Medicine and Hygiene 24, 96–100.CrossRefGoogle Scholar
Oriol, R., Williams, J. F., Esandi, Perez M. V. & Oriol, C. (1971). Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. American Journal of Tropical Medicine and Hygiene 20, 569–74.CrossRefGoogle ScholarPubMed
Rickard, M. D., Davies, C., Bout, D. T. & Smyth, J. D. (1977). Immunohistological localisation of two hydatid antigens (antigen 5 and antigen B) in the cyst wall, brood capsules and protoscoleces of Echinococcus granulosus (ovine and equine) and E. multilocularis using immunoperoxidase methods. Journal of Helminthology 51, 359–64.CrossRefGoogle Scholar
Smyth, J. D. & Davies, Z. (1974). In vitro culture of the strobilar stage of Echinococcus granulosus (sheep strain): a review of basic problems and results. International Journal for Parasitology 4, 631–44.CrossRefGoogle Scholar
Smyth, J. D., Morseth, D. J. & Smyth, M. M. (1969). Observations on nuclear secretions in the rostellar gland cells of Echinococcus granulosus (Cestoda). The Nucleus 12, 47–56.Google Scholar
Sternberger, L. A. (1974). Immunocytochemistry. Foundations of Immunology Series. (ed. Osler, A. G., and Weiss, L..) New Jersey: Prentice-Hall Incorporated.Google Scholar
Sternberger, L. A., Hardy, P. H., Cuculis, J. J. & Meyer, H. G. (1970). The unlabelled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen–antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in the identification of spirochetes. Journal of Histochemistry and Cytochemistry 18, 315–33.CrossRefGoogle ScholarPubMed
Varela-Diaz, V. M., Coltorti, E. A., Ricardes, M. I., Guisantes, J. A. & Yarzabal, L. A. (1974). The immunoelectrophoretic characterization of sheep hydatid cyst fluid antigens. American Journal of Tropical Medicine and Hygiene 23, 1092–6.CrossRefGoogle ScholarPubMed
Yarzabal, L. A., Dupas, H., Bout, D. & Capron, A. (1976). Echinococcus granulosus: the distribution of hydatid fluid antigens in the tissues of the larval stage. I. Localization of the specific antigen of hydatid fluid (antigen 5). Experimental Parasitology 40, 391–6.CrossRefGoogle Scholar
Yarzabal, L. A., Dupas, H., Bout, D., Naquira, F. & Capron, A. (1977). Echinococcus granulosus: the distribution of hydatid fluid antigens in the tissues of the larval stage. II. Localization of the thermostable lipoprotein of parasitic origin (antigen B). Experimental Parasitology 42, 115–20.CrossRefGoogle ScholarPubMed