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Trypanosoma cruzi: cell surface dynamics in trypomastigotes of different strains

Published online by Cambridge University Press:  18 November 2019

Roberta Ferreira Cura das Neves ,
Camila Marques Adade Open the ORCID record for Camila Marques Adade [Opens in a new window] ,
Anne Cristine Silva Fernandes ,
Angela Hampshire Lopes  and
Thaïs Souto-Padrón
Roberta Ferreira Cura das Neves
Affiliation:
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, 21941-902, RJ, Brazil
Camila Marques Adade*
Affiliation:
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, 21941-902, RJ, Brazil
Anne Cristine Silva Fernandes
Affiliation:
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, 21941-902, RJ, Brazil
Angela Hampshire Lopes
Affiliation:
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, 21941-902, RJ, Brazil
Thaïs Souto-Padrón
Affiliation:
Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, 21941-902, RJ, Brazil
*
Author for correspondence: Camila Marques Adade, E-mail: [email protected]
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Abstract

Capping and shedding of ectodomains in Trypanosoma cruzi may be triggered by different ligands. Here, we analysed the mobility and shedding of cell surface components of living trypomastigotes of the Y strain and the CL Brener clone in the presence of poly-L-lysine, cationized ferritin (CF) and Concanavalin A (Con A). Poly-L-lysine and CF caused intense shedding in Y strain parasites. Shedding was less intense in CL Brener trypomastigotes, and approximately 10% of these parasites did not show any decrease in poly L-lysine or CF labelling. Binding of Con A induced low-intensity shedding in Y strain and redistribution of Con A-binding sites in CL Brener parasites. Trypomastigotes of the Y strain showed intense labelling with anti-〈-galactosyl antibodies, resulting in the lysis of approximately 30% of their population, in contrast with what was observed in CL Brener parasites. Incubation with Con A and CF protected trypomastigotes of the Y strain from lysis by anti-αGal. The last treatment did not interfere with the survival of the CL Brener parasites. This study corroborates with the idea that a ligand can differentially modulate the cell surface of T. cruzi, depending on the strain used, resulting in variable immune system responses and recognition by host cells.

Keywords

Cationized ferritincell surface modulationCon AsheddingTrypanosoma cruzi
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 310 - 321
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Copyright
Copyright © Cambridge University Press 2019

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Footnotes

*

In memorian.

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