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Transgenesis of schistosomes: approaches employing mobile genetic elements

Published online by Cambridge University Press:  09 November 2007

V. H. MANN ,
M. E. MORALES ,
K. J. KINES  and
P. J. BRINDLEY
V. H. MANN*
Affiliation:
Department of Tropical Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA
M. E. MORALES
Affiliation:
Department of Tropical Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA
K. J. KINES
Affiliation:
Department of Tropical Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA
P. J. BRINDLEY
Affiliation:
Department of Tropical Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA
*
*Corresponding author. Tel: +1 504 988 6619. Fax: +1 504 988 6686. E-mail: [email protected]
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Summary

Draft genome sequences for Schistosoma mansoni and Schistosoma japonicum are now available. However, the identity and importance of most schistosome genes have yet to be determined. Recently, progress has been made towards the genetic manipulation and transgenesis of schistosomes. Both loss-of-function and gain-of-function approaches appear to be feasible in schistosomes based on findings described in the past 5 years. This review focuses on reports of schistosome transgenesis, specifically those dealing with the transformation of schistosomes with exogenous mobile genetic elements and/or their endogenous relatives for the genetic manipulation of schistosomes. Transgenesis mediated by mobile genetic elements offers a potentially tractable route to introduce foreign genes to schistosomes, a means to determine the importance of schistosome genes, including those that could be targeted in novel interventions and the potential to undertake large-scale forward genetics by insertional mutagenesis.

Keywords

schistosometransgenesiselectroporationreporter geneRNA interferencepromotergene manipulationfunctional genomics
Type
Review Article
Information
Parasitology , Volume 135 , Issue 2 , February 2008 , pp. 141 - 153
Copyright
Copyright © Cambridge University Press 2007

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