Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-28T02:36:48.940Z Has data issue: false hasContentIssue false
  • English
  • Français

Temporal differences in praziquantel- and oxamniquine-induced tegumental damage to adult Schistosoma mansoni: implications for drug-antibody synergy

Published online by Cambridge University Press:  06 April 2009

P. G. Fallon ,
R. E. Fookes  and
G. A. Wharton
P. G. Fallon
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
R. E. Fookes
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
G. A. Wharton
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
Get access Rights & Permissions [Opens in a new window]

Summary

A temporal study of the effects on the tegument of Schistosoma mansoni adult worm following in vivo praziquantel and oxamniquine treatment was performed. Drug-induced damage to the tegument, exposure of surface antigens and attachment of host antibody occurred rapidly, within 1 h, following praziquantel treatment. Oxamniquine-treated worms required 4–8 days for these effects to be apparent. The 2 drugs differed in the degree and sites of damage on the worm surface. The administration of 2 different polyspecific rabbit sera with drug significantly increased the efficacy of praziquantel when administered with the drug, but not when given 6–9 days after drug treatment. In contrast, only 1 serum was synergistic with oxamniquine when administered with drug and both sera were synergistic when given 6–9 days after drug treatment. The effect of immune killing of drug-treated worms is discussed.

Keywords

Schistosoma mansonipraziquanteloxamniquinetegumental damageantigen exposuredrug-antibody synergy
Type
Research Article
Information
Parasitology , Volume 112 , Issue 1 , January 1996 , pp. 47 - 58
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abramowicz, M. (1992). Drugs for parasitic diseases. The Medical Letter 34, 17–26.Google Scholar
Amin, A. M. & Mikhail, E. G. (1989). Schistosoma mansoni: tegumental surface alterations following oxamniquine treatment of infected mice. Journal of the Egyptian Society of Parasitology 19 (Suppl.), 815–26.Google Scholar
Cioli, D., Pica-Mattoccia, L. & Archer, S. (1993). Drug resistance in schistosomes. Parasitology Today 9, 162–6.CrossRefGoogle ScholarPubMed
Day, T. A., Bennett, J. L. & Pax, R. A. (1992). Praziquantel: the enigmatic antiparasitic. Parasitology Today 8, 342–4.CrossRefGoogle ScholarPubMed
Doenhoff, M. J., Modha, J. & Lambertucci, J. R. (1988). Anti-schistosome chemotherapy enhanced by antibodies specific for a parasite esterase. Immunology 65, 507–10.Google ScholarPubMed
Doenhoff, M. J., Sabah, A. A., Fletcher, C., Webbe, G. & Bain, J. (1987). Evidence for an immune-dependent action of praziquantel on Schistosoma mansoni in mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 947–51.CrossRefGoogle ScholarPubMed
Fallon, P. G., Cooper, R. O, Probert, A. J. & Doenhoff, M. J. (1992). Immune-dependent chemotherapy of schistosomiasis. Parasitology 105, S41–S48.CrossRefGoogle ScholarPubMed
Fallon, P. G. & Doenhoff, M. J. (1995). Active immunisation of mice with Schistosoma mansoni worm membrane antigens enhances efficacy of praziquantel. Parasite Immunology 17, 261–8.CrossRefGoogle ScholarPubMed
Fallon, P. G., Hamilton, J. V. & Doenhoff, M. J. (1995). Efficacy of treatment of murine Schistosoma mansoni infections with praziquantel and oxamniquine correlates with infection intensity: role of host antibody. Parasitology 111, 59–66.Google Scholar
Fallon, P. G., McNeice, C., Probert, A. J. & Doenhoff, M. J. (1994 b). Quantification of praziquantel-induced damage on the surface of adult Schistosoma mansoni worms: estimation of esterase and alkaline phosphatase activity. Parasitology Research 80, 623–5.CrossRefGoogle ScholarPubMed
Fallon, P. G., Smith, P., Nicholls, T., Modha, J. & Doenhoff, M. J. (1994 a). Praziquantel-induced exposure of Schistosoma mansoni alkaline phosphatase: drug-antibody synergy which acts preferentially against female worms. Parasite Immunology 16, 529–35.CrossRefGoogle ScholarPubMed
Harnett, W. & Kusel, J. R. (1986). Increased exposure of parasite antigens at the surface of adult male Schistosoma mansoni exposed to praziquantel in vitro. Parasitology 93, 401–5.CrossRefGoogle ScholarPubMed
Kohn, A., Lopez-Alvarez, M. L. & Katz, N. (1982). Transmission and scanning electron microscopical studies in the tegument of male Schistosoma mansoni after oxamniquine treatment. Annales de Parasitologie Humaine et Comparée 57, 285–91.CrossRefGoogle ScholarPubMed
Lambertucci, J. R., Modha, J. & Doenhoff, M. J. (1989). Schistosoma mansoni: the therapeutic efficacy of oxamniquine is enhanced by immune serum. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 362–3.CrossRefGoogle ScholarPubMed
Mehlhorn, H., Becker, B., Andrews, P., Thomas, H. & Frenkel, J. K. (1981). In vivo and in vitro experiments on the effects of praziquantel on Schistosoma mansoni: a light and electron microscopic study. tArzneimittel-Forschung 31, 544–54.Google ScholarPubMed
Modha, J., Lambertucci, J. R., Doenhoff, M. J. & McLaren, D. J. (1990). Immune dependence of schistosomicidal chemotherapy: an ultrastructural study of Schistosoma mansoni infected adult worms exposed to praziquantel and immune serum in vivo. Parasite Immunology 12, 321–34.CrossRefGoogle ScholarPubMed
Sabah, A. A., Fletcher, C., Webbe, G. & Doenhoff, M. J. (1985). Schistosoma mansoni: reduced efficacy of chemotherapy in infected T-cell deprived mice. Experimental Parasitology 60, 348–54.CrossRefGoogle ScholarPubMed
Shalaby, I. M. I., Banaja, A. A. & Ghandour, A. M. (1991). Scanning electron microscopy of the tegumental surface of in vivo treated Schistosoma mansoni (Saudi Arabian geographic strain) with oxamniquine and praziquantel. Journal of the Egyptian Society of Parasitology 21, 797–814.Google Scholar
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed