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Tegumental disruption with Triton X-100 and its effects on longitudinal muscle function in male Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

J. W. Depenbusch ,
D. P. Thompson ,
R. A. Pax  and
J. L. Bennett
J. W. Depenbusch
Affiliation:
Department of Zoology, Pharmacology and Toxicology and Neurosciences Program, Michigan State University, East Lansing, Michigan 48824
D. P. Thompson
Affiliation:
Department of Zoology, Pharmacology and Toxicology and Neurosciences Program, Michigan State University, East Lansing, Michigan 48824
R. A. Pax
Affiliation:
Department of Zoology, Pharmacology and Toxicology and Neurosciences Program, Michigan State University, East Lansing, Michigan 48824
J. L. Bennett
Affiliation:
Department of Zoology, Pharmacology and Toxicology and Neurosciences Program, Michigan State University, East Lansing, Michigan 48824
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Summary

Longitudinal muscles in adult male Schistosoma mansoni appear to remain in almost all respects functional after tegumental disruption by Triton X-100. Responses to high K+, ouabain, low temperature, praziquantel and neurotransmitters are still present. Muscle membrane potentials remain near those of control animals and lanthanum nitrate is still excluded from the muscle after the Triton treatment. Although muscle contractility remains after tegumental disruption, sodium and calcium levels, as measured by plasma emission spectroscopy, increase by 20–30% while potassium and magnesium levels decrease by 15%. 45Ca2+ accumulation is double that of control animals. Upon exposure to a bathing medium lacking Ca2+, Triton-treated animals lose responsiveness to high K+, ouabain, low temperature and praziquantel more rapidly than do control animals. D-600, which effectively blocks the high K+ response in control animals, has no measurable effect on the high K+ response in Triton-treated animals, suggesting that voltage-dependent Ca2+ channels are present in the tegument.

Type
Research Article
Information
Parasitology , Volume 87 , Issue 1 , August 1983 , pp. 61 - 73
Copyright
Copyright © Cambridge University Press 1983

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References

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